Effects of low-level laser therapy on human osteoblastic cells grown on titanium

Effects of low-level laser therapy on human osteoblastic cells grown on titanium

The aim of this study was to investigate the effects of low-level laser therapy (LLLT) by using gallium aluminum arsenide (GaAlAs) diode laser on human osteoblastic cells grown on titanium (Ti). Osteoblastic cells were obtained by enzymatic digestion of human alveolar bone and cultured on Ti discs for up to 17 days. Cells were exposed to LLLT at 3 J/cm2 (wavelength of 780 nm) at days 3 and 7 and non-irradiated cultures were used as control. LLLT treatment did not influence culture growth, ALP activity, and mineralized matrix formation. Analysis of cultures by epifluorescence microscopy revealed an area without cells in LLLT treated cultures, which was repopulated latter with proliferative and less differentiated cells. Gene expression of ALP, OC, BSP, and BMP-7 was higher in LLLT treated cultures, while Runx2, OPN, and OPG were lower. These results indicate that LLLT modulates cell responses in a complex way stimulating osteoblastic differentiation, which suggests possible benefits on implant osseointegration despite a transient deleterious effect immediately after laser irradiation.

Saved in:
Bibliographic Details
Main Authors: Petri,Alice Dias, Teixeira,Lucas Novaes, Crippa,Grasiele Edilaine, Beloti,Marcio Mateus, Oliveira,Paulo Tambasco de, Rosa,Adalberto Luiz
Format: Digital revista
Language:English
Published: Fundação Odontológica de Ribeirão Preto 2010
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402010000600003
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S0103-64402010000600003
record_format ojs
spelling oai:scielo:S0103-644020100006000032011-01-17Effects of low-level laser therapy on human osteoblastic cells grown on titaniumPetri,Alice DiasTeixeira,Lucas NovaesCrippa,Grasiele EdilaineBeloti,Marcio MateusOliveira,Paulo Tambasco deRosa,Adalberto Luiz Bone laser osteoblast osseointegration titanium The aim of this study was to investigate the effects of low-level laser therapy (LLLT) by using gallium aluminum arsenide (GaAlAs) diode laser on human osteoblastic cells grown on titanium (Ti). Osteoblastic cells were obtained by enzymatic digestion of human alveolar bone and cultured on Ti discs for up to 17 days. Cells were exposed to LLLT at 3 J/cm2 (wavelength of 780 nm) at days 3 and 7 and non-irradiated cultures were used as control. LLLT treatment did not influence culture growth, ALP activity, and mineralized matrix formation. Analysis of cultures by epifluorescence microscopy revealed an area without cells in LLLT treated cultures, which was repopulated latter with proliferative and less differentiated cells. Gene expression of ALP, OC, BSP, and BMP-7 was higher in LLLT treated cultures, while Runx2, OPN, and OPG were lower. These results indicate that LLLT modulates cell responses in a complex way stimulating osteoblastic differentiation, which suggests possible benefits on implant osseointegration despite a transient deleterious effect immediately after laser irradiation.info:eu-repo/semantics/openAccessFundação Odontológica de Ribeirão PretoBrazilian Dental Journal v.21 n.6 20102010-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402010000600003en10.1590/S0103-64402010000600003
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Petri,Alice Dias
Teixeira,Lucas Novaes
Crippa,Grasiele Edilaine
Beloti,Marcio Mateus
Oliveira,Paulo Tambasco de
Rosa,Adalberto Luiz
spellingShingle Petri,Alice Dias
Teixeira,Lucas Novaes
Crippa,Grasiele Edilaine
Beloti,Marcio Mateus
Oliveira,Paulo Tambasco de
Rosa,Adalberto Luiz
Effects of low-level laser therapy on human osteoblastic cells grown on titanium
author_facet Petri,Alice Dias
Teixeira,Lucas Novaes
Crippa,Grasiele Edilaine
Beloti,Marcio Mateus
Oliveira,Paulo Tambasco de
Rosa,Adalberto Luiz
author_sort Petri,Alice Dias
title Effects of low-level laser therapy on human osteoblastic cells grown on titanium
title_short Effects of low-level laser therapy on human osteoblastic cells grown on titanium
title_full Effects of low-level laser therapy on human osteoblastic cells grown on titanium
title_fullStr Effects of low-level laser therapy on human osteoblastic cells grown on titanium
title_full_unstemmed Effects of low-level laser therapy on human osteoblastic cells grown on titanium
title_sort effects of low-level laser therapy on human osteoblastic cells grown on titanium
description The aim of this study was to investigate the effects of low-level laser therapy (LLLT) by using gallium aluminum arsenide (GaAlAs) diode laser on human osteoblastic cells grown on titanium (Ti). Osteoblastic cells were obtained by enzymatic digestion of human alveolar bone and cultured on Ti discs for up to 17 days. Cells were exposed to LLLT at 3 J/cm2 (wavelength of 780 nm) at days 3 and 7 and non-irradiated cultures were used as control. LLLT treatment did not influence culture growth, ALP activity, and mineralized matrix formation. Analysis of cultures by epifluorescence microscopy revealed an area without cells in LLLT treated cultures, which was repopulated latter with proliferative and less differentiated cells. Gene expression of ALP, OC, BSP, and BMP-7 was higher in LLLT treated cultures, while Runx2, OPN, and OPG were lower. These results indicate that LLLT modulates cell responses in a complex way stimulating osteoblastic differentiation, which suggests possible benefits on implant osseointegration despite a transient deleterious effect immediately after laser irradiation.
publisher Fundação Odontológica de Ribeirão Preto
publishDate 2010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402010000600003
work_keys_str_mv AT petrialicedias effectsoflowlevellasertherapyonhumanosteoblasticcellsgrownontitanium
AT teixeiralucasnovaes effectsoflowlevellasertherapyonhumanosteoblasticcellsgrownontitanium
AT crippagrasieleedilaine effectsoflowlevellasertherapyonhumanosteoblasticcellsgrownontitanium
AT belotimarciomateus effectsoflowlevellasertherapyonhumanosteoblasticcellsgrownontitanium
AT oliveirapaulotambascode effectsoflowlevellasertherapyonhumanosteoblasticcellsgrownontitanium
AT rosaadalbertoluiz effectsoflowlevellasertherapyonhumanosteoblasticcellsgrownontitanium
_version_ 1756404491356209152

Similar Items