Extraction and Quantitative HPLC Analysis of Coumarin in Hydroalcoholic Extracts of Mikania glomerata Spreng: ("guaco") Leaves
Methods for preparation of hydroalcoholic extracts of "guaco" (Mikania glomerata Spreng.) leaves were compared: maceration, maceration under sonication, infusion and supercritical fluid extraction. Evaluation of these methods showed that maceration under sonication had the best results, when considering the ratio extraction yield/extraction time. A high performance liquid chromatography (HPLC) procedure for the determination of coumarin in these hydroalcoholic extracts of "guaco" leaves is described. The HPLC method is shown to be sensitive and reproducible.
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Main Authors: | Celeghini,Renata M. S., Vilegas,Janete H. Y., Lanças,Fernando M. |
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Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Química
2001
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Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532001000600003 |
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