Evaluation of the therapeutic effect of methylene blue on the liver of rats submitted to ischemia and reperfusion
Abstract Purpose: To analyze the effect of methylene blue (MB) therapy during the liver ischemia-reperfusion injury (I/R) process. Methods: Thirty-five male Wistar rats were used, (70%) submitted to partial ischemia (IR) or not (NIR) (30%) were obtained from the same animal. These animals were divided into six groups: 1) Sham (SH), 2) Sham with MB (SH-MB); 3) I/R, submitted to 60 minutes of partial ischemia and 15 minutes of reperfusion; 4) NI/R, without I/R obtained from the same animal of group I/R; 5) I/R-MB submitted to I/R and MB and 6) NI/R-MB, without I/R. Mitochondrial function was evaluated. Osmotic swelling of mitochondria as well as the determination of malondialdehyde (MDA) was evaluated. Serum (ALT/AST) dosages were also performed. MB was used at the concentration of 15mg/kg, 15 minutes before hepatic reperfusion. Statistical analysis was done by the Mann Whitney test at 5%. Results: State 3 shows inhibition in all ischemic groups. State 4 was increased in all groups, except the I/R-MB and NI/R-MB groups. RCR showed a decrease in all I/R and NI/R groups. Mitochondrial osmotic swelling showed an increase in all I/R NI/R groups in the presence or absence of MB. About MDA, there was a decrease in SH values in the presence of MB and this decrease was maintained in the I/R group. AST levels were increased in all ischemic with or without MB. Conclusions: The methylene blue was not able to restore the mitochondrial parameters studied. Also, it was able to decrease lipid peroxidation, preventing the formation of reactive oxygen species.
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2018
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oai:scielo:S0102-865020180012010432019-01-03Evaluation of the therapeutic effect of methylene blue on the liver of rats submitted to ischemia and reperfusionCastro-e-Silva,OrlandoD’Albuquerque,Luiz Augusto CarneiroSilveira,Marina Rodrigues GarciaZorzi,PatriciaLiu,Jordan BistafaCampos,Daniel Tófoli QueirozVictorino,João PauloJordani,Maria CecíliaMendes,Karina Dal SassoÉvora,Paulo Roberto Barbosa Ischemia Reperfusion Methylene Blue Mitochondria Liver Rats Abstract Purpose: To analyze the effect of methylene blue (MB) therapy during the liver ischemia-reperfusion injury (I/R) process. Methods: Thirty-five male Wistar rats were used, (70%) submitted to partial ischemia (IR) or not (NIR) (30%) were obtained from the same animal. These animals were divided into six groups: 1) Sham (SH), 2) Sham with MB (SH-MB); 3) I/R, submitted to 60 minutes of partial ischemia and 15 minutes of reperfusion; 4) NI/R, without I/R obtained from the same animal of group I/R; 5) I/R-MB submitted to I/R and MB and 6) NI/R-MB, without I/R. Mitochondrial function was evaluated. Osmotic swelling of mitochondria as well as the determination of malondialdehyde (MDA) was evaluated. Serum (ALT/AST) dosages were also performed. MB was used at the concentration of 15mg/kg, 15 minutes before hepatic reperfusion. Statistical analysis was done by the Mann Whitney test at 5%. Results: State 3 shows inhibition in all ischemic groups. State 4 was increased in all groups, except the I/R-MB and NI/R-MB groups. RCR showed a decrease in all I/R and NI/R groups. Mitochondrial osmotic swelling showed an increase in all I/R NI/R groups in the presence or absence of MB. About MDA, there was a decrease in SH values in the presence of MB and this decrease was maintained in the I/R group. AST levels were increased in all ischemic with or without MB. Conclusions: The methylene blue was not able to restore the mitochondrial parameters studied. Also, it was able to decrease lipid peroxidation, preventing the formation of reactive oxygen species.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.33 n.12 20182018-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018001201043en10.1590/s0102-865020180120000001 |
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Castro-e-Silva,Orlando D’Albuquerque,Luiz Augusto Carneiro Silveira,Marina Rodrigues Garcia Zorzi,Patricia Liu,Jordan Bistafa Campos,Daniel Tófoli Queiroz Victorino,João Paulo Jordani,Maria Cecília Mendes,Karina Dal Sasso Évora,Paulo Roberto Barbosa |
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Castro-e-Silva,Orlando D’Albuquerque,Luiz Augusto Carneiro Silveira,Marina Rodrigues Garcia Zorzi,Patricia Liu,Jordan Bistafa Campos,Daniel Tófoli Queiroz Victorino,João Paulo Jordani,Maria Cecília Mendes,Karina Dal Sasso Évora,Paulo Roberto Barbosa Evaluation of the therapeutic effect of methylene blue on the liver of rats submitted to ischemia and reperfusion |
author_facet |
Castro-e-Silva,Orlando D’Albuquerque,Luiz Augusto Carneiro Silveira,Marina Rodrigues Garcia Zorzi,Patricia Liu,Jordan Bistafa Campos,Daniel Tófoli Queiroz Victorino,João Paulo Jordani,Maria Cecília Mendes,Karina Dal Sasso Évora,Paulo Roberto Barbosa |
author_sort |
Castro-e-Silva,Orlando |
title |
Evaluation of the therapeutic effect of methylene blue on the liver of rats submitted to ischemia and reperfusion |
title_short |
Evaluation of the therapeutic effect of methylene blue on the liver of rats submitted to ischemia and reperfusion |
title_full |
Evaluation of the therapeutic effect of methylene blue on the liver of rats submitted to ischemia and reperfusion |
title_fullStr |
Evaluation of the therapeutic effect of methylene blue on the liver of rats submitted to ischemia and reperfusion |
title_full_unstemmed |
Evaluation of the therapeutic effect of methylene blue on the liver of rats submitted to ischemia and reperfusion |
title_sort |
evaluation of the therapeutic effect of methylene blue on the liver of rats submitted to ischemia and reperfusion |
description |
Abstract Purpose: To analyze the effect of methylene blue (MB) therapy during the liver ischemia-reperfusion injury (I/R) process. Methods: Thirty-five male Wistar rats were used, (70%) submitted to partial ischemia (IR) or not (NIR) (30%) were obtained from the same animal. These animals were divided into six groups: 1) Sham (SH), 2) Sham with MB (SH-MB); 3) I/R, submitted to 60 minutes of partial ischemia and 15 minutes of reperfusion; 4) NI/R, without I/R obtained from the same animal of group I/R; 5) I/R-MB submitted to I/R and MB and 6) NI/R-MB, without I/R. Mitochondrial function was evaluated. Osmotic swelling of mitochondria as well as the determination of malondialdehyde (MDA) was evaluated. Serum (ALT/AST) dosages were also performed. MB was used at the concentration of 15mg/kg, 15 minutes before hepatic reperfusion. Statistical analysis was done by the Mann Whitney test at 5%. Results: State 3 shows inhibition in all ischemic groups. State 4 was increased in all groups, except the I/R-MB and NI/R-MB groups. RCR showed a decrease in all I/R and NI/R groups. Mitochondrial osmotic swelling showed an increase in all I/R NI/R groups in the presence or absence of MB. About MDA, there was a decrease in SH values in the presence of MB and this decrease was maintained in the I/R group. AST levels were increased in all ischemic with or without MB. Conclusions: The methylene blue was not able to restore the mitochondrial parameters studied. Also, it was able to decrease lipid peroxidation, preventing the formation of reactive oxygen species. |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
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2018 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018001201043 |
work_keys_str_mv |
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