Optimization of total polyphenol extraction and flavonoid screening by mass spectrometry in mango (Mangifera indica L.) waste from Peru

Abstract This research aims to optimize the extraction yield of total phenolic compounds (TPC) and quantify flavonoids by mass spectrometry in peel and kernel of mango (Mangifera indica L.), varieties: Edward, Kent, Haden, and Criollo from the department of Lambayeque, Peru, which resulted in eight samples. Mango peels and kernels were manually separated, frozen at -20 °C, freeze-dried, and ground (300 μm). For the extraction, the Central Composite Design was applied with the factors of ethanolic solution, time, and sample/volume ratio. The extracts determined TPCs by Folin-Ciocalteu and UV-Vis spectrophotometry expressed as gallic acid equivalent. Optimization was performed by the desirability function; Quercetin was also quantified by liquid chromatography-mass spectrometry (m/z). The highest yield of TPC content for Criollo mango kernel was obtained with 67.99% ethanolic solution, 89.94 min, and 0.343 g sample/10 mL ethanolic solution with R2 of 0.8966, and for Edward mango peel with 73.996% ethanolic solution, 58.5 min, and 0.432 g sample/10 mL ethanolic solution with R2 of 0.8020. For peel, the methanolic extract from Criollo mango peel had the highest Quercetin value at (23.28 ± 2.35 mg QE/100 g) (p < 0.05), and for kernels, in both extractions (ethanolic and methanolic), the four varieties did not present differences (p > 0.05).

Saved in:
Bibliographic Details
Main Authors: LEÓN-ROQUE,Noemí, ROMERO GUZMÁN,Blanca Margarita, OBLITAS-CRUZ,Jimy Frank, HIDALGO-CHÁVEZ,Davy William
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Ciência e Tecnologia de Alimentos 2023
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612023000100445
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S0101-20612023000100445
record_format ojs
spelling oai:scielo:S0101-206120230001004452022-12-20Optimization of total polyphenol extraction and flavonoid screening by mass spectrometry in mango (Mangifera indica L.) waste from PeruLEÓN-ROQUE,NoemíROMERO GUZMÁN,Blanca MargaritaOBLITAS-CRUZ,Jimy FrankHIDALGO-CHÁVEZ,Davy William kernel peel desirability polyphenols Quercetin spectrometry untargeted metabolomic Abstract This research aims to optimize the extraction yield of total phenolic compounds (TPC) and quantify flavonoids by mass spectrometry in peel and kernel of mango (Mangifera indica L.), varieties: Edward, Kent, Haden, and Criollo from the department of Lambayeque, Peru, which resulted in eight samples. Mango peels and kernels were manually separated, frozen at -20 °C, freeze-dried, and ground (300 μm). For the extraction, the Central Composite Design was applied with the factors of ethanolic solution, time, and sample/volume ratio. The extracts determined TPCs by Folin-Ciocalteu and UV-Vis spectrophotometry expressed as gallic acid equivalent. Optimization was performed by the desirability function; Quercetin was also quantified by liquid chromatography-mass spectrometry (m/z). The highest yield of TPC content for Criollo mango kernel was obtained with 67.99% ethanolic solution, 89.94 min, and 0.343 g sample/10 mL ethanolic solution with R2 of 0.8966, and for Edward mango peel with 73.996% ethanolic solution, 58.5 min, and 0.432 g sample/10 mL ethanolic solution with R2 of 0.8020. For peel, the methanolic extract from Criollo mango peel had the highest Quercetin value at (23.28 ± 2.35 mg QE/100 g) (p < 0.05), and for kernels, in both extractions (ethanolic and methanolic), the four varieties did not present differences (p > 0.05).info:eu-repo/semantics/openAccessSociedade Brasileira de Ciência e Tecnologia de AlimentosFood Science and Technology v.43 20232023-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612023000100445en10.1590/fst.105322
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author LEÓN-ROQUE,Noemí
ROMERO GUZMÁN,Blanca Margarita
OBLITAS-CRUZ,Jimy Frank
HIDALGO-CHÁVEZ,Davy William
spellingShingle LEÓN-ROQUE,Noemí
ROMERO GUZMÁN,Blanca Margarita
OBLITAS-CRUZ,Jimy Frank
HIDALGO-CHÁVEZ,Davy William
Optimization of total polyphenol extraction and flavonoid screening by mass spectrometry in mango (Mangifera indica L.) waste from Peru
author_facet LEÓN-ROQUE,Noemí
ROMERO GUZMÁN,Blanca Margarita
OBLITAS-CRUZ,Jimy Frank
HIDALGO-CHÁVEZ,Davy William
author_sort LEÓN-ROQUE,Noemí
title Optimization of total polyphenol extraction and flavonoid screening by mass spectrometry in mango (Mangifera indica L.) waste from Peru
title_short Optimization of total polyphenol extraction and flavonoid screening by mass spectrometry in mango (Mangifera indica L.) waste from Peru
title_full Optimization of total polyphenol extraction and flavonoid screening by mass spectrometry in mango (Mangifera indica L.) waste from Peru
title_fullStr Optimization of total polyphenol extraction and flavonoid screening by mass spectrometry in mango (Mangifera indica L.) waste from Peru
title_full_unstemmed Optimization of total polyphenol extraction and flavonoid screening by mass spectrometry in mango (Mangifera indica L.) waste from Peru
title_sort optimization of total polyphenol extraction and flavonoid screening by mass spectrometry in mango (mangifera indica l.) waste from peru
description Abstract This research aims to optimize the extraction yield of total phenolic compounds (TPC) and quantify flavonoids by mass spectrometry in peel and kernel of mango (Mangifera indica L.), varieties: Edward, Kent, Haden, and Criollo from the department of Lambayeque, Peru, which resulted in eight samples. Mango peels and kernels were manually separated, frozen at -20 °C, freeze-dried, and ground (300 μm). For the extraction, the Central Composite Design was applied with the factors of ethanolic solution, time, and sample/volume ratio. The extracts determined TPCs by Folin-Ciocalteu and UV-Vis spectrophotometry expressed as gallic acid equivalent. Optimization was performed by the desirability function; Quercetin was also quantified by liquid chromatography-mass spectrometry (m/z). The highest yield of TPC content for Criollo mango kernel was obtained with 67.99% ethanolic solution, 89.94 min, and 0.343 g sample/10 mL ethanolic solution with R2 of 0.8966, and for Edward mango peel with 73.996% ethanolic solution, 58.5 min, and 0.432 g sample/10 mL ethanolic solution with R2 of 0.8020. For peel, the methanolic extract from Criollo mango peel had the highest Quercetin value at (23.28 ± 2.35 mg QE/100 g) (p < 0.05), and for kernels, in both extractions (ethanolic and methanolic), the four varieties did not present differences (p > 0.05).
publisher Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publishDate 2023
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612023000100445
work_keys_str_mv AT leonroquenoemi optimizationoftotalpolyphenolextractionandflavonoidscreeningbymassspectrometryinmangomangiferaindicalwastefromperu
AT romeroguzmanblancamargarita optimizationoftotalpolyphenolextractionandflavonoidscreeningbymassspectrometryinmangomangiferaindicalwastefromperu
AT oblitascruzjimyfrank optimizationoftotalpolyphenolextractionandflavonoidscreeningbymassspectrometryinmangomangiferaindicalwastefromperu
AT hidalgochavezdavywilliam optimizationoftotalpolyphenolextractionandflavonoidscreeningbymassspectrometryinmangomangiferaindicalwastefromperu
_version_ 1756472318815633408