Sinningia allagophylla(Gesneriaceae): in vitro cultivation of a native plant of the Brazilian cerrado
We used axillary buds as initial explants for hormone interaction studies required for in vitro cultivation of S. allagophylla. Callus production was achieved on gelled Murashige & Skoog medium (MS) supplemented with indole-3-acetic acid (IAA= 0.1 and 0.5 mg.l1 alone or combined with 6 benzylaminopurine) (BA= 0.01 and 0.1 mg.l-1). A hormone balance between IAA and BA that would encourage shoot bud development was not found. Nodal segments from axenic cultures grown in the presence of cytokinin (0.1 mg.11 of BA) without any auxin on MS medium with half-strength macronutrients were used as a standard explant source for subsequent experiments on optimum mineral culture media composition for S. allagophylla in vitro cultivation. We found that explants kept in vitro on gelled Gamborg et al. (B5) mineral composition culture medium showed better shoot and specially root growth than on MS medium. Comparisons of the ammonium and nitrate ratios of MS and B5 media indicate that B5 medium has a substantial reduced ammonium ion when compared to MS medium, as well as a lower total nitrogen level. The growth response pattern obtained in vitro may be evidence of the adaptation of this species to soils of poor mineral composition as found in the Brazilian cerrado, as well as an indication that nitrogen levels play a key role for S. allagophylla growth.
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Sociedade Botânica de São Paulo
1999
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oai:scielo:S0100-840419990003000052000-10-09Sinningia allagophylla(Gesneriaceae): in vitro cultivation of a native plant of the Brazilian cerradoALMEIDA,VILMA PALAZETTI DESHEPHERD,SIMONE LILIANE KIRSZENZAFT Cerrado plant tissue culture growth regulators nitrogen levels We used axillary buds as initial explants for hormone interaction studies required for in vitro cultivation of S. allagophylla. Callus production was achieved on gelled Murashige & Skoog medium (MS) supplemented with indole-3-acetic acid (IAA= 0.1 and 0.5 mg.l1 alone or combined with 6 benzylaminopurine) (BA= 0.01 and 0.1 mg.l-1). A hormone balance between IAA and BA that would encourage shoot bud development was not found. Nodal segments from axenic cultures grown in the presence of cytokinin (0.1 mg.11 of BA) without any auxin on MS medium with half-strength macronutrients were used as a standard explant source for subsequent experiments on optimum mineral culture media composition for S. allagophylla in vitro cultivation. We found that explants kept in vitro on gelled Gamborg et al. (B5) mineral composition culture medium showed better shoot and specially root growth than on MS medium. Comparisons of the ammonium and nitrate ratios of MS and B5 media indicate that B5 medium has a substantial reduced ammonium ion when compared to MS medium, as well as a lower total nitrogen level. The growth response pattern obtained in vitro may be evidence of the adaptation of this species to soils of poor mineral composition as found in the Brazilian cerrado, as well as an indication that nitrogen levels play a key role for S. allagophylla growth.info:eu-repo/semantics/openAccessSociedade Botânica de São PauloBrazilian Journal of Botany v.22 n.3 19991999-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84041999000300005en10.1590/S0100-84041999000300005 |
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ALMEIDA,VILMA PALAZETTI DE SHEPHERD,SIMONE LILIANE KIRSZENZAFT |
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ALMEIDA,VILMA PALAZETTI DE SHEPHERD,SIMONE LILIANE KIRSZENZAFT Sinningia allagophylla(Gesneriaceae): in vitro cultivation of a native plant of the Brazilian cerrado |
author_facet |
ALMEIDA,VILMA PALAZETTI DE SHEPHERD,SIMONE LILIANE KIRSZENZAFT |
author_sort |
ALMEIDA,VILMA PALAZETTI DE |
title |
Sinningia allagophylla(Gesneriaceae): in vitro cultivation of a native plant of the Brazilian cerrado |
title_short |
Sinningia allagophylla(Gesneriaceae): in vitro cultivation of a native plant of the Brazilian cerrado |
title_full |
Sinningia allagophylla(Gesneriaceae): in vitro cultivation of a native plant of the Brazilian cerrado |
title_fullStr |
Sinningia allagophylla(Gesneriaceae): in vitro cultivation of a native plant of the Brazilian cerrado |
title_full_unstemmed |
Sinningia allagophylla(Gesneriaceae): in vitro cultivation of a native plant of the Brazilian cerrado |
title_sort |
sinningia allagophylla(gesneriaceae): in vitro cultivation of a native plant of the brazilian cerrado |
description |
We used axillary buds as initial explants for hormone interaction studies required for in vitro cultivation of S. allagophylla. Callus production was achieved on gelled Murashige & Skoog medium (MS) supplemented with indole-3-acetic acid (IAA= 0.1 and 0.5 mg.l1 alone or combined with 6 benzylaminopurine) (BA= 0.01 and 0.1 mg.l-1). A hormone balance between IAA and BA that would encourage shoot bud development was not found. Nodal segments from axenic cultures grown in the presence of cytokinin (0.1 mg.11 of BA) without any auxin on MS medium with half-strength macronutrients were used as a standard explant source for subsequent experiments on optimum mineral culture media composition for S. allagophylla in vitro cultivation. We found that explants kept in vitro on gelled Gamborg et al. (B5) mineral composition culture medium showed better shoot and specially root growth than on MS medium. Comparisons of the ammonium and nitrate ratios of MS and B5 media indicate that B5 medium has a substantial reduced ammonium ion when compared to MS medium, as well as a lower total nitrogen level. The growth response pattern obtained in vitro may be evidence of the adaptation of this species to soils of poor mineral composition as found in the Brazilian cerrado, as well as an indication that nitrogen levels play a key role for S. allagophylla growth. |
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Sociedade Botânica de São Paulo |
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1999 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84041999000300005 |
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AT almeidavilmapalazettide sinningiaallagophyllagesneriaceaeinvitrocultivationofanativeplantofthebraziliancerrado AT shepherdsimonelilianekirszenzaft sinningiaallagophyllagesneriaceaeinvitrocultivationofanativeplantofthebraziliancerrado |
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