Enterotoxin-encoding genes in Staphylococcus aureus from buffalo milk

ABSTRACT: This paper investigated the occurrence of Staphylococcus aureus and the detection of enterotoxin-encoding genes of these strains in milk collected from 30 Murrah buffaloes used to produce dairy products in Brazil. A total of 68 strains of Staphylococcus aureus were found as identified by conventional laboratory tests, and thus screened for sea, seb, sec, sed, see, seg, seh and sei enterotoxin-encoding genes by polymerase chain reaction (PCR). Twelve strains containing enterotoxin-amplified genes were found, with higher expression for the sei and seh genes. These results can be attributed to animal health and inadequate cleaning of the equipment, indicating the need for better quality control in animal production and health lines. The results of this study with the presence of pathogens and their enterotoxigenic potential indicate a source of food poisoning, as well as being a pioneering study in the detection of new enterotoxins for buffalo milk.

Bibliographic Details

Main Authors:	Moura,Emmanuella O., Rangel,Adriano H.N., Macêdo,Cláudia S., Urbano,Stela A., Novaes,Luciano P., Lima Júnior,Dorgival M.
Format:	Digital revista
Language:	English
Published:	Colégio Brasileiro de Patologia Animal - CBPA 2019
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2019000800587
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