Prevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyoderma

Prevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyoderma

ABSTRACT: In order to assess the prevalence of Methicillin-resistant Staphylococcus pseudintermedius from skin and nostrils of dogs with pyoderma, to determine its in vitro susceptibility, and to correlate these data with the presence of the mecA gene, 43 dogs were selected. Samples were collected from secretion of their skin lesions and right nostril, cultured, and analyzed for phenotypic and genotypic characteristics of the bacteria studied. In 62 samples (91%) the microorganism was classified as S. pseudintermedius. The rate of resistance against antibiotics ranged from 7% (amikacin; 4/62) to 77% (sulfamethoxazole + trimethoprim; 48/62). Resistance against oxacillin was found in 34% of the samples (21/62). Twenty-five samples (37%) were strains that carried the mecA gene. A significant correlation (P<0.01) was found between presence of the mecA gene and oxacillin resistance. Seventeen dogs were mecA gene carriers, and 8 (47%) of them had the gene in the skin lesions and nostril. A significant correlation (P<0.01) was also observed between the presence of mecA gene in the skin lesions and nostrils. Oxacillin resistance in vitro can be safely used to indicate the presence of mecA gene in MRSP samples. The nostrils can be a reservoir of MRSP in dogs.

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Main Authors: Botoni,Larissa S., Scherer,Carolina B., Silva,Rodrigo O., Coura,Fernanda M., Heinemann,Marcos B., Paes-Leme,Fabiola O., Costa-Val,Adriane P.
Format: Digital revista
Language:English
Published: Colégio Brasileiro de Patologia Animal - CBPA 2016
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016001201178
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spelling oai:scielo:S0100-736X20160012011782017-03-02Prevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyodermaBotoni,Larissa S.Scherer,Carolina B.Silva,Rodrigo O.Coura,Fernanda M.Heinemann,Marcos B.Paes-Leme,Fabiola O.Costa-Val,Adriane P. Skin disease Staphylococcus pseudintermedius staphylococcal infections antibiotics oxacillin mecA MRSP dogs. ABSTRACT: In order to assess the prevalence of Methicillin-resistant Staphylococcus pseudintermedius from skin and nostrils of dogs with pyoderma, to determine its in vitro susceptibility, and to correlate these data with the presence of the mecA gene, 43 dogs were selected. Samples were collected from secretion of their skin lesions and right nostril, cultured, and analyzed for phenotypic and genotypic characteristics of the bacteria studied. In 62 samples (91%) the microorganism was classified as S. pseudintermedius. The rate of resistance against antibiotics ranged from 7% (amikacin; 4/62) to 77% (sulfamethoxazole + trimethoprim; 48/62). Resistance against oxacillin was found in 34% of the samples (21/62). Twenty-five samples (37%) were strains that carried the mecA gene. A significant correlation (P<0.01) was found between presence of the mecA gene and oxacillin resistance. Seventeen dogs were mecA gene carriers, and 8 (47%) of them had the gene in the skin lesions and nostril. A significant correlation (P<0.01) was also observed between the presence of mecA gene in the skin lesions and nostrils. Oxacillin resistance in vitro can be safely used to indicate the presence of mecA gene in MRSP samples. The nostrils can be a reservoir of MRSP in dogs.info:eu-repo/semantics/openAccessColégio Brasileiro de Patologia Animal - CBPAPesquisa Veterinária Brasileira v.36 n.12 20162016-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016001201178en10.1590/s0100-736x2016001200006
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language English
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author Botoni,Larissa S.
Scherer,Carolina B.
Silva,Rodrigo O.
Coura,Fernanda M.
Heinemann,Marcos B.
Paes-Leme,Fabiola O.
Costa-Val,Adriane P.
spellingShingle Botoni,Larissa S.
Scherer,Carolina B.
Silva,Rodrigo O.
Coura,Fernanda M.
Heinemann,Marcos B.
Paes-Leme,Fabiola O.
Costa-Val,Adriane P.
Prevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyoderma
author_facet Botoni,Larissa S.
Scherer,Carolina B.
Silva,Rodrigo O.
Coura,Fernanda M.
Heinemann,Marcos B.
Paes-Leme,Fabiola O.
Costa-Val,Adriane P.
author_sort Botoni,Larissa S.
title Prevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyoderma
title_short Prevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyoderma
title_full Prevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyoderma
title_fullStr Prevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyoderma
title_full_unstemmed Prevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyoderma
title_sort prevalence and in vitro susceptibility of methicillin-resistant staphylococcus pseudintermedius (mrsp) from skin and nostrils of dogs with superficial pyoderma
description ABSTRACT: In order to assess the prevalence of Methicillin-resistant Staphylococcus pseudintermedius from skin and nostrils of dogs with pyoderma, to determine its in vitro susceptibility, and to correlate these data with the presence of the mecA gene, 43 dogs were selected. Samples were collected from secretion of their skin lesions and right nostril, cultured, and analyzed for phenotypic and genotypic characteristics of the bacteria studied. In 62 samples (91%) the microorganism was classified as S. pseudintermedius. The rate of resistance against antibiotics ranged from 7% (amikacin; 4/62) to 77% (sulfamethoxazole + trimethoprim; 48/62). Resistance against oxacillin was found in 34% of the samples (21/62). Twenty-five samples (37%) were strains that carried the mecA gene. A significant correlation (P<0.01) was found between presence of the mecA gene and oxacillin resistance. Seventeen dogs were mecA gene carriers, and 8 (47%) of them had the gene in the skin lesions and nostril. A significant correlation (P<0.01) was also observed between the presence of mecA gene in the skin lesions and nostrils. Oxacillin resistance in vitro can be safely used to indicate the presence of mecA gene in MRSP samples. The nostrils can be a reservoir of MRSP in dogs.
publisher Colégio Brasileiro de Patologia Animal - CBPA
publishDate 2016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016001201178
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