Simultaneous determination of gemifloxacin and diuretics in bulk, pharmaceutical dosage forms and human serum by RP-HPLC
An isocratic reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the simultaneous determination of gemifloxacin and diuretics (hydrochlorothiazide and furosemide) in bulk, dosage formulations and human serum at 232 nm. Chromatographic separation was achieved on Purospher Start C18 (250 mm x 4.6 mm, 5 µm) column using mobile phase, methanol: water: acetonitrile (70:25:5 v/v/v) adjusted to pH 3.0 via phosphoric acid 85% having flow rate of 0.8 mL min -1 at room temperature. Calibration curves were linear over range of 0.5-10 µg mL -1 with a correlation coefficient ± 0.999. LOD and LOQ were in the ranges of 0.75-2.56 µg mL -1. Intra and inter-run precision and accuracy results were 98.26 to 100.9.
Saved in:
Main Authors: | Sultana,Najma, Shamim,Sana, Gul,Mahwish Akhtarand Somia, Arayne,M. Saeed |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Química
2010
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000700029 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Validated method for the determination of Gemifloxacin in bulk, pharmaceutical formulations and human serum by RP-HPLC: in vitro applications
by: Sultana,Najma, et al.
Published: (2011) -
Simultaneous determination of moxifloxacin and H2 receptor antagonist in pharmaceutical dosage formulations by RP-HPLC: application to in vitro drug interactions
by: Sultana,Najma, et al.
Published: (2011) -
SIMULTANEOUS DETERMINATION OF ENALAPRIL AND STATIN'S IN PHARMACEUTICAL FORMULATIONS BY RP-HPLC
by: SULTANA,NAJMA, et al.
Published: (2011) -
SIMULTANEOUS DETERMINATION OF CEFTRIAXONE SODIUM AND STATIN DRUGS IN PHARMACEUTICAL FORMULATIONS AND HUMAN SERUM BY RP-HPLC
by: SULTANA,NAJMA, et al.
Published: (2010) -
A RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF DILTIAZEM AND QUINOLONES IN BULK, FORMULATIONS AND HUMAN SERUM
by: SULTANA,NAJMA, et al.
Published: (2009)