Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity
This opinion piece presents an approach to standardisation of an important aspect of Chagas disease drug discovery and development: selecting Trypanosoma cruzi strains for in vitro screening. We discuss the rationale for strain selection representing T. cruzi diversity and provide recommendations on the preferred parasite stage for drug discovery, T. cruzi discrete typing units to include in the panel of strains and the number of strains/clones for primary screens and lead compounds. We also consider experimental approaches for in vitro drug assays. The Figure illustrates the current Chagas disease drug-discovery and development landscape.
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Format: | Digital revista |
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Instituto Oswaldo Cruz, Ministério da Saúde
2014
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oai:scielo:S0074-027620140006008282014-10-07Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversityZingales,BiancaMiles,Michael AMoraes,Carolina BLuquetti,AlejandroGuhl,FelipeSchijman,Alejandro GRibeiro,Isabela in vitro drug screening T. cruzi genetic diversity guidelines This opinion piece presents an approach to standardisation of an important aspect of Chagas disease drug discovery and development: selecting Trypanosoma cruzi strains for in vitro screening. We discuss the rationale for strain selection representing T. cruzi diversity and provide recommendations on the preferred parasite stage for drug discovery, T. cruzi discrete typing units to include in the panel of strains and the number of strains/clones for primary screens and lead compounds. We also consider experimental approaches for in vitro drug assays. The Figure illustrates the current Chagas disease drug-discovery and development landscape.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.109 n.6 20142014-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000600828en10.1590/0074-0276140156 |
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Zingales,Bianca Miles,Michael A Moraes,Carolina B Luquetti,Alejandro Guhl,Felipe Schijman,Alejandro G Ribeiro,Isabela |
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Zingales,Bianca Miles,Michael A Moraes,Carolina B Luquetti,Alejandro Guhl,Felipe Schijman,Alejandro G Ribeiro,Isabela Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity |
author_facet |
Zingales,Bianca Miles,Michael A Moraes,Carolina B Luquetti,Alejandro Guhl,Felipe Schijman,Alejandro G Ribeiro,Isabela |
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Zingales,Bianca |
title |
Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity |
title_short |
Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity |
title_full |
Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity |
title_fullStr |
Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity |
title_full_unstemmed |
Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity |
title_sort |
drug discovery for chagas disease should consider trypanosoma cruzi strain diversity |
description |
This opinion piece presents an approach to standardisation of an important aspect of Chagas disease drug discovery and development: selecting Trypanosoma cruzi strains for in vitro screening. We discuss the rationale for strain selection representing T. cruzi diversity and provide recommendations on the preferred parasite stage for drug discovery, T. cruzi discrete typing units to include in the panel of strains and the number of strains/clones for primary screens and lead compounds. We also consider experimental approaches for in vitro drug assays. The Figure illustrates the current Chagas disease drug-discovery and development landscape. |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2014 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000600828 |
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