Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection

Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection

Proline racemase is an important enzyme of Trypanosoma cruzi and has been shown to be an effective mitogen for B cells, thus contributing to the parasite's immune evasion and persistence in the human host. Recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro. Here we demonstrate that both anti T. cruzi proline racemase antibodies and the specific proline racemase inhibitor pyrrole-2-carboxylic acid significantly affect parasite infection of Vero cells in vitro. This inhibitor also hampers T. cruzi intracellular differentiation.

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Main Authors: Coutinho,Leticia, Ferreira,Marcelo Alves, Cosson,Alain, Batista,Marcos Meuser, Batista,Denise da Gama Jaén, Minoprio,Paola, Degrave,Wim M, Berneman,Armand, Soeiro,Maria de Nazaré Correia
Format: Digital revista
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2009
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000800001
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spelling oai:scielo:S0074-027620090008000012010-02-22Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infectionCoutinho,LeticiaFerreira,Marcelo AlvesCosson,AlainBatista,Marcos MeuserBatista,Denise da Gama JaénMinoprio,PaolaDegrave,Wim MBerneman,ArmandSoeiro,Maria de Nazaré Correia Trypanosoma cruzi proline racemase host-parasite interaction enzyme inhibition Proline racemase is an important enzyme of Trypanosoma cruzi and has been shown to be an effective mitogen for B cells, thus contributing to the parasite's immune evasion and persistence in the human host. Recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro. Here we demonstrate that both anti T. cruzi proline racemase antibodies and the specific proline racemase inhibitor pyrrole-2-carboxylic acid significantly affect parasite infection of Vero cells in vitro. This inhibitor also hampers T. cruzi intracellular differentiation.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.104 n.8 20092009-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000800001en10.1590/S0074-02762009000800001
institution SCIELO
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country Brasil
countrycode BR
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access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Coutinho,Leticia
Ferreira,Marcelo Alves
Cosson,Alain
Batista,Marcos Meuser
Batista,Denise da Gama Jaén
Minoprio,Paola
Degrave,Wim M
Berneman,Armand
Soeiro,Maria de Nazaré Correia
spellingShingle Coutinho,Leticia
Ferreira,Marcelo Alves
Cosson,Alain
Batista,Marcos Meuser
Batista,Denise da Gama Jaén
Minoprio,Paola
Degrave,Wim M
Berneman,Armand
Soeiro,Maria de Nazaré Correia
Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
author_facet Coutinho,Leticia
Ferreira,Marcelo Alves
Cosson,Alain
Batista,Marcos Meuser
Batista,Denise da Gama Jaén
Minoprio,Paola
Degrave,Wim M
Berneman,Armand
Soeiro,Maria de Nazaré Correia
author_sort Coutinho,Leticia
title Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
title_short Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
title_full Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
title_fullStr Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
title_full_unstemmed Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
title_sort inhibition of trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
description Proline racemase is an important enzyme of Trypanosoma cruzi and has been shown to be an effective mitogen for B cells, thus contributing to the parasite's immune evasion and persistence in the human host. Recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro. Here we demonstrate that both anti T. cruzi proline racemase antibodies and the specific proline racemase inhibitor pyrrole-2-carboxylic acid significantly affect parasite infection of Vero cells in vitro. This inhibitor also hampers T. cruzi intracellular differentiation.
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 2009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000800001
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