Methodological limitations of counting total leukocytes and thrombocytes in reptiles (Amazon turtle, Podocnemis expansa): an analysis and discussion
The aim of this paper is to compare three different methods for counting white blood cells [WBC] (Natt and Herrick method, estimation with 1,000 and 2,000 erythrocytes) and three methods for counting total thrombocytes [TT] (Wojtaszek method, estimation with 1,000 and 2,000 erythrocytes) in a South American freshwater turtle species, Podocnemis expansa, Schweigger 1812 (Reptilia, Pelomedusidae). Direct WBC counts using the Natt and Herrick method showed limitations, which are discussed here. The WBC and TT counts using 1,000 erythrocytes from blood smears are not recommended for Amazon turtles nor other reptilian species, since wide variation in counts can be observed. Estimation methods for determining WBC and TT based on 2,000 erythrocytes of blood smears were most acceptable because they allow a differentiation between leukocytes and thrombocytes and also had a smaller variation. The methods investigated here for the Amazon turtle, which have been widely used in other reptile species, provided evidence that the most acceptable method is not that of using diluted stains and a hemocytometer.
Main Authors: | , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Instituto Nacional de Pesquisas da Amazônia
2008
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000200020 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S0044-59672008000200020 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S0044-596720080002000202008-08-01Methodological limitations of counting total leukocytes and thrombocytes in reptiles (Amazon turtle, Podocnemis expansa): an analysis and discussionTavares-Dias,MarcosOliveira-Júnior,Antônio AdersonMarcon,Jaydione Luiz Amazon turtles Blood leukocytes Podocnemis expansa Thrombocytes The aim of this paper is to compare three different methods for counting white blood cells [WBC] (Natt and Herrick method, estimation with 1,000 and 2,000 erythrocytes) and three methods for counting total thrombocytes [TT] (Wojtaszek method, estimation with 1,000 and 2,000 erythrocytes) in a South American freshwater turtle species, Podocnemis expansa, Schweigger 1812 (Reptilia, Pelomedusidae). Direct WBC counts using the Natt and Herrick method showed limitations, which are discussed here. The WBC and TT counts using 1,000 erythrocytes from blood smears are not recommended for Amazon turtles nor other reptilian species, since wide variation in counts can be observed. Estimation methods for determining WBC and TT based on 2,000 erythrocytes of blood smears were most acceptable because they allow a differentiation between leukocytes and thrombocytes and also had a smaller variation. The methods investigated here for the Amazon turtle, which have been widely used in other reptile species, provided evidence that the most acceptable method is not that of using diluted stains and a hemocytometer.info:eu-repo/semantics/openAccessInstituto Nacional de Pesquisas da AmazôniaActa Amazonica v.38 n.2 20082008-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000200020en10.1590/S0044-59672008000200020 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Tavares-Dias,Marcos Oliveira-Júnior,Antônio Aderson Marcon,Jaydione Luiz |
spellingShingle |
Tavares-Dias,Marcos Oliveira-Júnior,Antônio Aderson Marcon,Jaydione Luiz Methodological limitations of counting total leukocytes and thrombocytes in reptiles (Amazon turtle, Podocnemis expansa): an analysis and discussion |
author_facet |
Tavares-Dias,Marcos Oliveira-Júnior,Antônio Aderson Marcon,Jaydione Luiz |
author_sort |
Tavares-Dias,Marcos |
title |
Methodological limitations of counting total leukocytes and thrombocytes in reptiles (Amazon turtle, Podocnemis expansa): an analysis and discussion |
title_short |
Methodological limitations of counting total leukocytes and thrombocytes in reptiles (Amazon turtle, Podocnemis expansa): an analysis and discussion |
title_full |
Methodological limitations of counting total leukocytes and thrombocytes in reptiles (Amazon turtle, Podocnemis expansa): an analysis and discussion |
title_fullStr |
Methodological limitations of counting total leukocytes and thrombocytes in reptiles (Amazon turtle, Podocnemis expansa): an analysis and discussion |
title_full_unstemmed |
Methodological limitations of counting total leukocytes and thrombocytes in reptiles (Amazon turtle, Podocnemis expansa): an analysis and discussion |
title_sort |
methodological limitations of counting total leukocytes and thrombocytes in reptiles (amazon turtle, podocnemis expansa): an analysis and discussion |
description |
The aim of this paper is to compare three different methods for counting white blood cells [WBC] (Natt and Herrick method, estimation with 1,000 and 2,000 erythrocytes) and three methods for counting total thrombocytes [TT] (Wojtaszek method, estimation with 1,000 and 2,000 erythrocytes) in a South American freshwater turtle species, Podocnemis expansa, Schweigger 1812 (Reptilia, Pelomedusidae). Direct WBC counts using the Natt and Herrick method showed limitations, which are discussed here. The WBC and TT counts using 1,000 erythrocytes from blood smears are not recommended for Amazon turtles nor other reptilian species, since wide variation in counts can be observed. Estimation methods for determining WBC and TT based on 2,000 erythrocytes of blood smears were most acceptable because they allow a differentiation between leukocytes and thrombocytes and also had a smaller variation. The methods investigated here for the Amazon turtle, which have been widely used in other reptile species, provided evidence that the most acceptable method is not that of using diluted stains and a hemocytometer. |
publisher |
Instituto Nacional de Pesquisas da Amazônia |
publishDate |
2008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000200020 |
work_keys_str_mv |
AT tavaresdiasmarcos methodologicallimitationsofcountingtotalleukocytesandthrombocytesinreptilesamazonturtlepodocnemisexpansaananalysisanddiscussion AT oliveirajuniorantonioaderson methodologicallimitationsofcountingtotalleukocytesandthrombocytesinreptilesamazonturtlepodocnemisexpansaananalysisanddiscussion AT marconjaydioneluiz methodologicallimitationsofcountingtotalleukocytesandthrombocytesinreptilesamazonturtlepodocnemisexpansaananalysisanddiscussion |
_version_ |
1756381112593022976 |