Cacipacore virus as an emergent mosquito-borne Flavivirus

Cacipacore virus as an emergent mosquito-borne Flavivirus

Abstract INTRODUCTION: Cacipacore virus (CPCV), a possible bird-associated flavivirus, has yet to be detected in mosquitoes. Our purpose is examining CPCV in mosquitoes from the Amazon region of Brazil. METHODS: Approximately 3,253 Culicidae (grouped into 264 pools) were collected from the Amazon region during 2002-2006 and analyzed using a Flavivirus genus-specific reverse transcription- polymerase chain reaction followed by nested polymerase chain reaction assay and by nucleotide sequencing of amplicons. RESULTS: Nucleotide sequences from five mosquito samples showed high similarity to the those of CPCV originally isolated in the Amazon region. CONCLUSIONS: This is the first report of CPCV-infected mosquitoes which has implications on the arbovirus maintenance in nature and transmission to man.

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Bibliographic Details
Main Authors: Figueiredo,Mario Luis Garcia de, Amarilla,Alberto Anastacio, Figueiredo,Glauciane Garcia de, Alfonso,Helda Liz, Lippi,Veronica, Maia,Felipe Gonçalves Motta, Morais,Felipe Alves, Costa,Cristóvão Alves da, Henriques,Dyana Alves, Durigon,Edison Luis, Figueiredo,Luiz Tadeu Moraes, Aquino,Victor Hugo
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina Tropical - SBMT 2017
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017000400539
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Summary:Abstract INTRODUCTION: Cacipacore virus (CPCV), a possible bird-associated flavivirus, has yet to be detected in mosquitoes. Our purpose is examining CPCV in mosquitoes from the Amazon region of Brazil. METHODS: Approximately 3,253 Culicidae (grouped into 264 pools) were collected from the Amazon region during 2002-2006 and analyzed using a Flavivirus genus-specific reverse transcription- polymerase chain reaction followed by nested polymerase chain reaction assay and by nucleotide sequencing of amplicons. RESULTS: Nucleotide sequences from five mosquito samples showed high similarity to the those of CPCV originally isolated in the Amazon region. CONCLUSIONS: This is the first report of CPCV-infected mosquitoes which has implications on the arbovirus maintenance in nature and transmission to man.

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