Detection of bla KPC-2 in Proteus mirabilis in Brazil
INTRODUCTION : Infections caused by Klebsiella pneumoniae carbapenemase (KPC)-producing isolates pose a major worldwide public health problem today. METHODS : A carbapenem-resistant Proteus mirabilis clinical isolate was investigated for plasmid profiles and the occurrence of β-lactamase genes. RESULTS : The isolate exhibited resistance to ertapenem and imipenem and was susceptible to meropenem, polymyxin, and tigecycline. Five plasmids were identified in this isolate. DNA sequencing analysis revealed the presence of bla KPC-2 and bla TEM-1 genes. An additional PCR using plasmid DNA confirmed that bla KPC-2 was present in one of these plasmids. Conclusions: We report the detection of bla KPC-2 in P. mirabilis in Brazil for the first time. This finding highlights the continuous transfer of bla KPC between bacterial genera, which presents a serious challenge to the prevention of infection by multidrug-resistant bacteria.
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Sociedade Brasileira de Medicina Tropical - SBMT
2015
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oai:scielo:S0037-868220150001000942015-03-31Detection of bla KPC-2 in Proteus mirabilis in BrazilCabral,Adriane BorgesMaciel,Maria Amélia VieiraBarros,Josineide FerreiraAntunes,Marcelo MaranhãoLopes,Ana Catarina Souza Proteus mirabilis blaKPC-2 Plasmid DNA INTRODUCTION : Infections caused by Klebsiella pneumoniae carbapenemase (KPC)-producing isolates pose a major worldwide public health problem today. METHODS : A carbapenem-resistant Proteus mirabilis clinical isolate was investigated for plasmid profiles and the occurrence of β-lactamase genes. RESULTS : The isolate exhibited resistance to ertapenem and imipenem and was susceptible to meropenem, polymyxin, and tigecycline. Five plasmids were identified in this isolate. DNA sequencing analysis revealed the presence of bla KPC-2 and bla TEM-1 genes. An additional PCR using plasmid DNA confirmed that bla KPC-2 was present in one of these plasmids. Conclusions: We report the detection of bla KPC-2 in P. mirabilis in Brazil for the first time. This finding highlights the continuous transfer of bla KPC between bacterial genera, which presents a serious challenge to the prevention of infection by multidrug-resistant bacteria. info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.48 n.1 20152015-02-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000100094en10.1590/0037-8682-0152-2014 |
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Brasil |
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BR |
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Cabral,Adriane Borges Maciel,Maria Amélia Vieira Barros,Josineide Ferreira Antunes,Marcelo Maranhão Lopes,Ana Catarina Souza |
| spellingShingle |
Cabral,Adriane Borges Maciel,Maria Amélia Vieira Barros,Josineide Ferreira Antunes,Marcelo Maranhão Lopes,Ana Catarina Souza Detection of bla KPC-2 in Proteus mirabilis in Brazil |
| author_facet |
Cabral,Adriane Borges Maciel,Maria Amélia Vieira Barros,Josineide Ferreira Antunes,Marcelo Maranhão Lopes,Ana Catarina Souza |
| author_sort |
Cabral,Adriane Borges |
| title |
Detection of bla KPC-2 in Proteus mirabilis in Brazil |
| title_short |
Detection of bla KPC-2 in Proteus mirabilis in Brazil |
| title_full |
Detection of bla KPC-2 in Proteus mirabilis in Brazil |
| title_fullStr |
Detection of bla KPC-2 in Proteus mirabilis in Brazil |
| title_full_unstemmed |
Detection of bla KPC-2 in Proteus mirabilis in Brazil |
| title_sort |
detection of bla kpc-2 in proteus mirabilis in brazil |
| description |
INTRODUCTION : Infections caused by Klebsiella pneumoniae carbapenemase (KPC)-producing isolates pose a major worldwide public health problem today. METHODS : A carbapenem-resistant Proteus mirabilis clinical isolate was investigated for plasmid profiles and the occurrence of β-lactamase genes. RESULTS : The isolate exhibited resistance to ertapenem and imipenem and was susceptible to meropenem, polymyxin, and tigecycline. Five plasmids were identified in this isolate. DNA sequencing analysis revealed the presence of bla KPC-2 and bla TEM-1 genes. An additional PCR using plasmid DNA confirmed that bla KPC-2 was present in one of these plasmids. Conclusions: We report the detection of bla KPC-2 in P. mirabilis in Brazil for the first time. This finding highlights the continuous transfer of bla KPC between bacterial genera, which presents a serious challenge to the prevention of infection by multidrug-resistant bacteria. |
| publisher |
Sociedade Brasileira de Medicina Tropical - SBMT |
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2015 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000100094 |
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