Evaluation of eleven immunochromatographic assays for SARS-CoV-2 detection: investigating the dengue cross-reaction
ABSTRACT COVID-19 disease is spread worldwide and diagnostic techniques have been studied in order to contain the pandemic. Immunochromatographic (IC) assays are feasible and a low-cost alternative especially in low and middle-income countries, which lack structure to perform certain diagnostic techniques. Here we evaluate the sensitivity and specificity of eleven different IC tests in 145 serum samples from confirmed cases of COVID-19 using RT-PCR and 100 negative serum samples from blood donors collected in February 2019. We also evaluated the cross-reactivity with dengue using 20 serum samples from patients with confirmed diagnosis for dengue collected in early 2019 through four different tests. We found high sensitivity (92%), specificity (100%) and an almost perfect agreement (Kappa 0.92) of IC assay, especially when we evaluated IgG and IgM combined after 10 days from the onset of symptoms with RT-PCR. However, we detected cross-reactivity between dengue and COVID-19 mainly with IgM antibodies (5 to 20% of cross-reaction) and demonstrated the need for better studies about diagnostic techniques for these diseases.
Main Authors: | Oliveira,Beatriz Araujo, Oliveira,Lea Campos de, Oliveira,Franciane Mendes de, Pereira,Geovana Maria, Souza,Regina Maia de, Manuli,Erika Regina, Marchini,Fabricio Klerynton, Espinoza,Evelyn Patrícia Sanchez, Park,Marcelo, Taniguchi,Leandro, Mendes,Pedro Vitale, Franco,Lucas Augusto Moyses, Nastri,Ana Catharina, Oliveira,Maura Salaroli de, Vieira Junior,José Mauro, Kallas,Esper Georges, Levin,Anna Sara, Sabino,Ester Cerdeira, Costa,Silvia Figueiredo |
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Format: | Digital revista |
Language: | English |
Published: |
Instituto de Medicina Tropical de São Paulo
2022
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Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652022000100234 |
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