Evaluation of eleven immunochromatographic assays for SARS-CoV-2 detection: investigating the dengue cross-reaction
ABSTRACT COVID-19 disease is spread worldwide and diagnostic techniques have been studied in order to contain the pandemic. Immunochromatographic (IC) assays are feasible and a low-cost alternative especially in low and middle-income countries, which lack structure to perform certain diagnostic techniques. Here we evaluate the sensitivity and specificity of eleven different IC tests in 145 serum samples from confirmed cases of COVID-19 using RT-PCR and 100 negative serum samples from blood donors collected in February 2019. We also evaluated the cross-reactivity with dengue using 20 serum samples from patients with confirmed diagnosis for dengue collected in early 2019 through four different tests. We found high sensitivity (92%), specificity (100%) and an almost perfect agreement (Kappa 0.92) of IC assay, especially when we evaluated IgG and IgM combined after 10 days from the onset of symptoms with RT-PCR. However, we detected cross-reactivity between dengue and COVID-19 mainly with IgM antibodies (5 to 20% of cross-reaction) and demonstrated the need for better studies about diagnostic techniques for these diseases.
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Instituto de Medicina Tropical de São Paulo
2022
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oai:scielo:S0036-466520220001002342022-09-27Evaluation of eleven immunochromatographic assays for SARS-CoV-2 detection: investigating the dengue cross-reactionOliveira,Beatriz AraujoOliveira,Lea Campos deOliveira,Franciane Mendes dePereira,Geovana MariaSouza,Regina Maia deManuli,Erika ReginaMarchini,Fabricio KleryntonEspinoza,Evelyn Patrícia SanchezPark,MarceloTaniguchi,LeandroMendes,Pedro VitaleFranco,Lucas Augusto MoysesNastri,Ana CatharinaOliveira,Maura Salaroli deVieira Junior,José MauroKallas,Esper GeorgesLevin,Anna SaraSabino,Ester CerdeiraCosta,Silvia Figueiredo IC assays COVID-19 Dengue Serology ABSTRACT COVID-19 disease is spread worldwide and diagnostic techniques have been studied in order to contain the pandemic. Immunochromatographic (IC) assays are feasible and a low-cost alternative especially in low and middle-income countries, which lack structure to perform certain diagnostic techniques. Here we evaluate the sensitivity and specificity of eleven different IC tests in 145 serum samples from confirmed cases of COVID-19 using RT-PCR and 100 negative serum samples from blood donors collected in February 2019. We also evaluated the cross-reactivity with dengue using 20 serum samples from patients with confirmed diagnosis for dengue collected in early 2019 through four different tests. We found high sensitivity (92%), specificity (100%) and an almost perfect agreement (Kappa 0.92) of IC assay, especially when we evaluated IgG and IgM combined after 10 days from the onset of symptoms with RT-PCR. However, we detected cross-reactivity between dengue and COVID-19 mainly with IgM antibodies (5 to 20% of cross-reaction) and demonstrated the need for better studies about diagnostic techniques for these diseases.info:eu-repo/semantics/openAccessInstituto de Medicina Tropical de São PauloRevista do Instituto de Medicina Tropical de São Paulo v.64 20222022-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652022000100234en10.1590/s1678-9946202264063 |
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Oliveira,Beatriz Araujo Oliveira,Lea Campos de Oliveira,Franciane Mendes de Pereira,Geovana Maria Souza,Regina Maia de Manuli,Erika Regina Marchini,Fabricio Klerynton Espinoza,Evelyn Patrícia Sanchez Park,Marcelo Taniguchi,Leandro Mendes,Pedro Vitale Franco,Lucas Augusto Moyses Nastri,Ana Catharina Oliveira,Maura Salaroli de Vieira Junior,José Mauro Kallas,Esper Georges Levin,Anna Sara Sabino,Ester Cerdeira Costa,Silvia Figueiredo |
spellingShingle |
Oliveira,Beatriz Araujo Oliveira,Lea Campos de Oliveira,Franciane Mendes de Pereira,Geovana Maria Souza,Regina Maia de Manuli,Erika Regina Marchini,Fabricio Klerynton Espinoza,Evelyn Patrícia Sanchez Park,Marcelo Taniguchi,Leandro Mendes,Pedro Vitale Franco,Lucas Augusto Moyses Nastri,Ana Catharina Oliveira,Maura Salaroli de Vieira Junior,José Mauro Kallas,Esper Georges Levin,Anna Sara Sabino,Ester Cerdeira Costa,Silvia Figueiredo Evaluation of eleven immunochromatographic assays for SARS-CoV-2 detection: investigating the dengue cross-reaction |
author_facet |
Oliveira,Beatriz Araujo Oliveira,Lea Campos de Oliveira,Franciane Mendes de Pereira,Geovana Maria Souza,Regina Maia de Manuli,Erika Regina Marchini,Fabricio Klerynton Espinoza,Evelyn Patrícia Sanchez Park,Marcelo Taniguchi,Leandro Mendes,Pedro Vitale Franco,Lucas Augusto Moyses Nastri,Ana Catharina Oliveira,Maura Salaroli de Vieira Junior,José Mauro Kallas,Esper Georges Levin,Anna Sara Sabino,Ester Cerdeira Costa,Silvia Figueiredo |
author_sort |
Oliveira,Beatriz Araujo |
title |
Evaluation of eleven immunochromatographic assays for SARS-CoV-2 detection: investigating the dengue cross-reaction |
title_short |
Evaluation of eleven immunochromatographic assays for SARS-CoV-2 detection: investigating the dengue cross-reaction |
title_full |
Evaluation of eleven immunochromatographic assays for SARS-CoV-2 detection: investigating the dengue cross-reaction |
title_fullStr |
Evaluation of eleven immunochromatographic assays for SARS-CoV-2 detection: investigating the dengue cross-reaction |
title_full_unstemmed |
Evaluation of eleven immunochromatographic assays for SARS-CoV-2 detection: investigating the dengue cross-reaction |
title_sort |
evaluation of eleven immunochromatographic assays for sars-cov-2 detection: investigating the dengue cross-reaction |
description |
ABSTRACT COVID-19 disease is spread worldwide and diagnostic techniques have been studied in order to contain the pandemic. Immunochromatographic (IC) assays are feasible and a low-cost alternative especially in low and middle-income countries, which lack structure to perform certain diagnostic techniques. Here we evaluate the sensitivity and specificity of eleven different IC tests in 145 serum samples from confirmed cases of COVID-19 using RT-PCR and 100 negative serum samples from blood donors collected in February 2019. We also evaluated the cross-reactivity with dengue using 20 serum samples from patients with confirmed diagnosis for dengue collected in early 2019 through four different tests. We found high sensitivity (92%), specificity (100%) and an almost perfect agreement (Kappa 0.92) of IC assay, especially when we evaluated IgG and IgM combined after 10 days from the onset of symptoms with RT-PCR. However, we detected cross-reactivity between dengue and COVID-19 mainly with IgM antibodies (5 to 20% of cross-reaction) and demonstrated the need for better studies about diagnostic techniques for these diseases. |
publisher |
Instituto de Medicina Tropical de São Paulo |
publishDate |
2022 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652022000100234 |
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