Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii

Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii

ABSTRACT Toxoplasma gondii can cross the placental barrier, causing fetal infection with potentially severe sequelae. The aim of this study was to evaluate whether the serological screening for toxoplasmosis should be included in the basic neonatal heel prick test in order to establish criteria for the confirmation and/or exclusion of the diagnosis of congenital infection in newborns treated at three public health units in the metropolitan region of Goiania, Goias State, Brazil. Blood samples were collected on filter paper from newborns and later, peripheral blood samples from the mothers and their respective children were obtained to confirm or exclude the diagnosis of suspected congenital infection, by means of an enzyme-linked immunosorbent assay (IgM and IgG) and a polymerase chain reaction assay. From a total of 1,159 blood samples collected on filter paper, 43.92% were reactive to IgG and 0.17% to anti-T. gondii IgM and IgG. One hundred and twenty-seven paired samples (mother and child) were collected following consensual protocols for peripheral blood collection. Results obtained from the filter paper and peripheral blood of the newborns were 90.55% concordant. A comparison of the mother and child blood test results showed agreement regarding the detection of IgG in 90.48% of the samples. The parasite DNA was detected in the peripheral blood of one child. In view of the results obtained in this study, the inclusion of the serological screening for toxoplasmosis in the newborn heel prick test proved to be effective for the early detection of congenital T. gondii infection.

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Main Authors: Storchilo,Heloisa Ribeiro, Rezende,Hanstter Hallison Alves, Gomes,Taynara Cristina, Souza,Jéssica Yonara de, Gomes Junior,Antonio Roberto, Avelino,Mariza Martins, Amaral,Waldemar Naves do, Castro,Ana Maria de
Format: Digital revista
Language:English
Published: Instituto de Medicina Tropical de São Paulo 2019
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652019005000214
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spelling oai:scielo:S0036-466520190050002142019-06-17Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondiiStorchilo,Heloisa RibeiroRezende,Hanstter Hallison AlvesGomes,Taynara CristinaSouza,Jéssica Yonara deGomes Junior,Antonio RobertoAvelino,Mariza MartinsAmaral,Waldemar Naves doCastro,Ana Maria de Serological tests Neonatal screening Newborn Congenital toxoplasmosis Heel prick test ABSTRACT Toxoplasma gondii can cross the placental barrier, causing fetal infection with potentially severe sequelae. The aim of this study was to evaluate whether the serological screening for toxoplasmosis should be included in the basic neonatal heel prick test in order to establish criteria for the confirmation and/or exclusion of the diagnosis of congenital infection in newborns treated at three public health units in the metropolitan region of Goiania, Goias State, Brazil. Blood samples were collected on filter paper from newborns and later, peripheral blood samples from the mothers and their respective children were obtained to confirm or exclude the diagnosis of suspected congenital infection, by means of an enzyme-linked immunosorbent assay (IgM and IgG) and a polymerase chain reaction assay. From a total of 1,159 blood samples collected on filter paper, 43.92% were reactive to IgG and 0.17% to anti-T. gondii IgM and IgG. One hundred and twenty-seven paired samples (mother and child) were collected following consensual protocols for peripheral blood collection. Results obtained from the filter paper and peripheral blood of the newborns were 90.55% concordant. A comparison of the mother and child blood test results showed agreement regarding the detection of IgG in 90.48% of the samples. The parasite DNA was detected in the peripheral blood of one child. In view of the results obtained in this study, the inclusion of the serological screening for toxoplasmosis in the newborn heel prick test proved to be effective for the early detection of congenital T. gondii infection.info:eu-repo/semantics/openAccessInstituto de Medicina Tropical de São PauloRevista do Instituto de Medicina Tropical de São Paulo v.61 20192019-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652019005000214en10.1590/s1678-9946201961030
institution SCIELO
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country Brasil
countrycode BR
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language English
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author Storchilo,Heloisa Ribeiro
Rezende,Hanstter Hallison Alves
Gomes,Taynara Cristina
Souza,Jéssica Yonara de
Gomes Junior,Antonio Roberto
Avelino,Mariza Martins
Amaral,Waldemar Naves do
Castro,Ana Maria de
spellingShingle Storchilo,Heloisa Ribeiro
Rezende,Hanstter Hallison Alves
Gomes,Taynara Cristina
Souza,Jéssica Yonara de
Gomes Junior,Antonio Roberto
Avelino,Mariza Martins
Amaral,Waldemar Naves do
Castro,Ana Maria de
Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii
author_facet Storchilo,Heloisa Ribeiro
Rezende,Hanstter Hallison Alves
Gomes,Taynara Cristina
Souza,Jéssica Yonara de
Gomes Junior,Antonio Roberto
Avelino,Mariza Martins
Amaral,Waldemar Naves do
Castro,Ana Maria de
author_sort Storchilo,Heloisa Ribeiro
title Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii
title_short Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii
title_full Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii
title_fullStr Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii
title_full_unstemmed Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii
title_sort basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by toxoplasma gondii
description ABSTRACT Toxoplasma gondii can cross the placental barrier, causing fetal infection with potentially severe sequelae. The aim of this study was to evaluate whether the serological screening for toxoplasmosis should be included in the basic neonatal heel prick test in order to establish criteria for the confirmation and/or exclusion of the diagnosis of congenital infection in newborns treated at three public health units in the metropolitan region of Goiania, Goias State, Brazil. Blood samples were collected on filter paper from newborns and later, peripheral blood samples from the mothers and their respective children were obtained to confirm or exclude the diagnosis of suspected congenital infection, by means of an enzyme-linked immunosorbent assay (IgM and IgG) and a polymerase chain reaction assay. From a total of 1,159 blood samples collected on filter paper, 43.92% were reactive to IgG and 0.17% to anti-T. gondii IgM and IgG. One hundred and twenty-seven paired samples (mother and child) were collected following consensual protocols for peripheral blood collection. Results obtained from the filter paper and peripheral blood of the newborns were 90.55% concordant. A comparison of the mother and child blood test results showed agreement regarding the detection of IgG in 90.48% of the samples. The parasite DNA was detected in the peripheral blood of one child. In view of the results obtained in this study, the inclusion of the serological screening for toxoplasmosis in the newborn heel prick test proved to be effective for the early detection of congenital T. gondii infection.
publisher Instituto de Medicina Tropical de São Paulo
publishDate 2019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652019005000214
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