Genotypic identification of Cryptosporidium spp. isolated from hiv-infected patients and immunocompetent children of São Paulo, Brazil
Cryptosporidium isolates identified in fourteen stool samples, collected from five HIV-infected patients and nine immunocompetent children, living in the Sate of São Paulo, Brazil, were submitted to a molecular analysis using a nested PCR followed of restriction fragment length polymorphism (RFLP), for genetic characterization. The analysis was based on digestion with RsaI restriction enzyme of a DNA fragment amplified from the Cryptosporidium oocyst wall protein (COWP) gene. Based on this analysis, four samples were identified as Cryptosporidium parvum, eight as Cryptosporidium hominis and two presented a profile that correspondedto Cryptosporidium meleagridis when compared to the standards used in the analysis. The use of molecular methods can be helpful to identify source of infections and risk factors related to Cryptosporidium infection in our communities.
Main Authors: | Araújo,Ana Julia Urias dos Santos, Kanamura,Herminia Yohko, Almeida,Marcos Eduardo de, Gomes,Aparecida Helena de Souza, Pinto,Thais Helena Lemos, Da Silva,Alexandre Januário |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Instituto de Medicina Tropical de São Paulo
2008
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652008000300002 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Detection of Cryptosporidium sp. in non diarrheal faeces from children, in a day care center in the city of São Paulo, Brazil
by: Carvalho-Almeida,Therezinha T., et al.
Published: (2006) -
Rapid Characterization of S. mansoni Expression Library Clones of Potential Interest
by: KANAMURA,Herminia Yohko, et al.
Published: (1997) -
INTESTINAL AND PULMONARY INFECTION BY Cryptosporidium parvum IN TWO PATIENTS WITH HIV/AIDS
by: REINA,Fábio Tadeu Rodrigues, et al.
Published: (2016) -
Isolated cerebral mucormycosis masquerading as a tumor in an immunocompetent patient
by: Chandra,Smita, et al.
Published: (2021) -
Genotype identification and gene isolation
by: 109258 Rao, R., et al.
Published: (1992)