Genotypic identification of Cryptosporidium spp. isolated from hiv-infected patients and immunocompetent children of São Paulo, Brazil

Cryptosporidium isolates identified in fourteen stool samples, collected from five HIV-infected patients and nine immunocompetent children, living in the Sate of São Paulo, Brazil, were submitted to a molecular analysis using a nested PCR followed of restriction fragment length polymorphism (RFLP), for genetic characterization. The analysis was based on digestion with RsaI restriction enzyme of a DNA fragment amplified from the Cryptosporidium oocyst wall protein (COWP) gene. Based on this analysis, four samples were identified as Cryptosporidium parvum, eight as Cryptosporidium hominis and two presented a profile that correspondedto Cryptosporidium meleagridis when compared to the standards used in the analysis. The use of molecular methods can be helpful to identify source of infections and risk factors related to Cryptosporidium infection in our communities.

Bibliographic Details

Main Authors:	Araújo,Ana Julia Urias dos Santos, Kanamura,Herminia Yohko, Almeida,Marcos Eduardo de, Gomes,Aparecida Helena de Souza, Pinto,Thais Helena Lemos, Da Silva,Alexandre Januário
Format:	Digital revista
Language:	English
Published:	Instituto de Medicina Tropical de São Paulo 2008
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652008000300002
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