Staining of intestinal protozoa with heidenhain's iron Hematoxylin

Staining of intestinal protozoa with heidenhain's iron Hematoxylin

Due to its unique properties, iron hematoxylin has been traditionally recommended for staining intestinal protozoa. This process can be simplified by reducing the number of steps and periods of permanence of the slides in some of the liquids used, without detriment to the quality of the results. Thus iron hematoxylin becomes adequate for routine use. Hematoxylin is a natural dye extracted from Haematoxylon campechianum, of the family Leguminosae. It must first be 'ripened', i.e. oxidized to hematein, which reacts with ferric ammonium sulphate to produce the ferric lake (iron hematoxylin), a basic dye. Iron hematoxylin most frequently stains regressively, i.e. the slides are first overstained and then differentiated.

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Bibliographic Details
Main Author: Ferreira,Claudio Santos
Format: Digital revista
Language:English
Published: Instituto de Medicina Tropical de São Paulo 2003
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652003000100009
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spelling oai:scielo:S0036-466520030001000092003-04-03Staining of intestinal protozoa with heidenhain's iron HematoxylinFerreira,Claudio Santos Iron hematoxylin staining Intestinal protozoa Due to its unique properties, iron hematoxylin has been traditionally recommended for staining intestinal protozoa. This process can be simplified by reducing the number of steps and periods of permanence of the slides in some of the liquids used, without detriment to the quality of the results. Thus iron hematoxylin becomes adequate for routine use. Hematoxylin is a natural dye extracted from Haematoxylon campechianum, of the family Leguminosae. It must first be 'ripened', i.e. oxidized to hematein, which reacts with ferric ammonium sulphate to produce the ferric lake (iron hematoxylin), a basic dye. Iron hematoxylin most frequently stains regressively, i.e. the slides are first overstained and then differentiated.info:eu-repo/semantics/openAccessInstituto de Medicina Tropical de São PauloRevista do Instituto de Medicina Tropical de São Paulo v.45 n.1 20032003-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652003000100009en10.1590/S0036-46652003000100009
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Ferreira,Claudio Santos
spellingShingle Ferreira,Claudio Santos
Staining of intestinal protozoa with heidenhain's iron Hematoxylin
author_facet Ferreira,Claudio Santos
author_sort Ferreira,Claudio Santos
title Staining of intestinal protozoa with heidenhain's iron Hematoxylin
title_short Staining of intestinal protozoa with heidenhain's iron Hematoxylin
title_full Staining of intestinal protozoa with heidenhain's iron Hematoxylin
title_fullStr Staining of intestinal protozoa with heidenhain's iron Hematoxylin
title_full_unstemmed Staining of intestinal protozoa with heidenhain's iron Hematoxylin
title_sort staining of intestinal protozoa with heidenhain's iron hematoxylin
description Due to its unique properties, iron hematoxylin has been traditionally recommended for staining intestinal protozoa. This process can be simplified by reducing the number of steps and periods of permanence of the slides in some of the liquids used, without detriment to the quality of the results. Thus iron hematoxylin becomes adequate for routine use. Hematoxylin is a natural dye extracted from Haematoxylon campechianum, of the family Leguminosae. It must first be 'ripened', i.e. oxidized to hematein, which reacts with ferric ammonium sulphate to produce the ferric lake (iron hematoxylin), a basic dye. Iron hematoxylin most frequently stains regressively, i.e. the slides are first overstained and then differentiated.
publisher Instituto de Medicina Tropical de São Paulo
publishDate 2003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652003000100009
work_keys_str_mv AT ferreiraclaudiosantos stainingofintestinalprotozoawithheidenhainsironhematoxylin
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