Enzyme linked immunosorbent assay: determination of anti-adenovirus antibodies in an infant population
In order to define an accurate assay for anti-adenovirus antibody detection, a recently developed ELISA was compared with IFA and CF. On 58 sera, the ELISA was more sensitive than both CF and IFA, which showed relative sensitivities of 63% and 94%, respectively. It was not possible to determine the exact specificity of the tests because of the lack of a gold standard. Furthermore, the ELISA was used to define the prevalence of adenovirus antibodies in 116 infants between 1 and 24 months old (mean 7.28). The data showed that maternal antibodies waned by the age of 5 to 6 months and that more than 80% of the children had been infected by adenoviruses by the age of 10 months.
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Instituto de Medicina Tropical de São Paulo
1989
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oai:scielo:S0036-466519890005000072010-11-17Enzyme linked immunosorbent assay: determination of anti-adenovirus antibodies in an infant populationWeinberg,AdrianaFink,Maria Cristina D. S.Takimoto,SuekoIshida,Maria AkikoSouza,Maria Cândida O. Adenovirus serology Enzyme linked immunosorbent assay Adenovirus infection in children In order to define an accurate assay for anti-adenovirus antibody detection, a recently developed ELISA was compared with IFA and CF. On 58 sera, the ELISA was more sensitive than both CF and IFA, which showed relative sensitivities of 63% and 94%, respectively. It was not possible to determine the exact specificity of the tests because of the lack of a gold standard. Furthermore, the ELISA was used to define the prevalence of adenovirus antibodies in 116 infants between 1 and 24 months old (mean 7.28). The data showed that maternal antibodies waned by the age of 5 to 6 months and that more than 80% of the children had been infected by adenoviruses by the age of 10 months.info:eu-repo/semantics/openAccessInstituto de Medicina Tropical de São PauloRevista do Instituto de Medicina Tropical de São Paulo v.31 n.5 19891989-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651989000500007en10.1590/S0036-46651989000500007 |
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Weinberg,Adriana Fink,Maria Cristina D. S. Takimoto,Sueko Ishida,Maria Akiko Souza,Maria Cândida O. |
| spellingShingle |
Weinberg,Adriana Fink,Maria Cristina D. S. Takimoto,Sueko Ishida,Maria Akiko Souza,Maria Cândida O. Enzyme linked immunosorbent assay: determination of anti-adenovirus antibodies in an infant population |
| author_facet |
Weinberg,Adriana Fink,Maria Cristina D. S. Takimoto,Sueko Ishida,Maria Akiko Souza,Maria Cândida O. |
| author_sort |
Weinberg,Adriana |
| title |
Enzyme linked immunosorbent assay: determination of anti-adenovirus antibodies in an infant population |
| title_short |
Enzyme linked immunosorbent assay: determination of anti-adenovirus antibodies in an infant population |
| title_full |
Enzyme linked immunosorbent assay: determination of anti-adenovirus antibodies in an infant population |
| title_fullStr |
Enzyme linked immunosorbent assay: determination of anti-adenovirus antibodies in an infant population |
| title_full_unstemmed |
Enzyme linked immunosorbent assay: determination of anti-adenovirus antibodies in an infant population |
| title_sort |
enzyme linked immunosorbent assay: determination of anti-adenovirus antibodies in an infant population |
| description |
In order to define an accurate assay for anti-adenovirus antibody detection, a recently developed ELISA was compared with IFA and CF. On 58 sera, the ELISA was more sensitive than both CF and IFA, which showed relative sensitivities of 63% and 94%, respectively. It was not possible to determine the exact specificity of the tests because of the lack of a gold standard. Furthermore, the ELISA was used to define the prevalence of adenovirus antibodies in 116 infants between 1 and 24 months old (mean 7.28). The data showed that maternal antibodies waned by the age of 5 to 6 months and that more than 80% of the children had been infected by adenoviruses by the age of 10 months. |
| publisher |
Instituto de Medicina Tropical de São Paulo |
| publishDate |
1989 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651989000500007 |
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1756379573050671104 |