Validation of multiplex PCR for the diagnosis of acute bacterial meningitis in culture negative cerebrospinal fluid
ABSTRACT This study evaluated the operational characteristics of the multiplex polymerase chain reaction (PCR) for cerebrospinal fluid (CSF) from patients with cellular and biochemical characteristics of acute bacterial meningitis and positive or negative CSF cultures. Methods: Multiplex PCR was performed for 36 CSF samples: culture-proven acute bacterial meningitis (n = 7), culture-negative acute bacterial meningitis (n = 17), lymphocytic meningitis (n = 8), and normal CSF (n = 4). The operational characteristics of multiplex PCR were evaluated with definite and probable bacterial meningitis, using culture positive, cytological and biochemical CSF characteristics as the gold standard. Results: Multiplex PCR for CSF was efficient in the group with CSF cellular and biochemical characteristics of acute bacterial meningitis but with a negative CSF culture. This group demonstrated high specificity, positive predictive value, and efficiency. Conclusions: Multiplex PCR for CSF can improve the speed and accuracy of acute bacterial meningitis diagnosis in a clinical setting as a complement to classical immunological and bacteriological assays in CSF. It is also useful for CSF culture-negative acute bacterial meningitis.
Main Authors: | de Almeida,Sérgio Monteiro, Dalla Costa,Libera Maria, Siebra,Christian, Arend,Lavinia Nery Villa Stangler, Nogueira,Keite da Silva |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Academia Brasileira de Neurologia - ABNEURO
2019
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2019000400224 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Comparison of cerebrospinal fluid lactate with physical, cytological, and other biochemical characteristics as prognostic factors in acute bacterial meningitis
by: De Almeida,Sérgio Monteiro, et al.
Published: (2019) -
Prediction of bacterial meningitis based on cerebrospinal fluid pleocytosis in children
by: Águeda,Sofia, et al.
Published: (2013) -
Comparison of enterovirus detection in cerebrospinal fluid with Bacterial Meningitis Score in children
by: Pires,Frederico Ribeiro, et al.
Published: (2017) -
Nosocomial meningitis caused by Klebsiella pneumoniae producing carbapenemase, with initial cerebrospinal fluid minimal inflammatory response
by: Almeida,Sérgio Monteiro de, et al.
Published: (2014) -
Cerebrospinal fluid lactate: a differential biomarker for bacterial and viral meningitis in children
by: Nazir,Mudasir, et al.
Published: (2018)