Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues, and long-term stored blood samples
OBJECTIVE: Adequate isolation of nucleic acids from peripheral blood, fine-needle aspiration cells in stained slides, and fresh and formalin-fixed/paraffin-embedded tissues is crucial to ensure the success of molecular endocrinology techniques, especially when samples are stored for long periods, or when no other samples can be collected from patients who are lost to follow-up. Here, we evaluate several procedures to improve current methodologies for DNA (salting-out) and RNA isolation. MATERIALS AND METHODS: We used proteinase K treatment, heat shock, and other adaptations to increase the amount and quality of the material retrieved from the samples. RESULTS: We successfully isolated DNA and RNA from the samples described above, and this material was suitable for PCR, methylation profiling, real-time PCR and DNA sequencing. CONCLUSION: The techniques herein applied to isolate nucleic acids allowed further reliable molecular analyses. Arq Bras Endocrinol Metab. 2012;56(9):618-26
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Sociedade Brasileira de Endocrinologia e Metabologia
2012
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oai:scielo:S0004-273020120009000042013-01-15Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues, and long-term stored blood samplesKizys,Marina M. L.Cardoso,Mirian G.Lindsey,Susan C.Harada,Michelle Y.Soares,Fernando A.Melo,Maria Clara C.Montoya,Marlyn Z.Kasamatsu,Teresa S.Kunii,Ilda S.Giannocco,GiseleMartins,João Roberto M.Cerutti,Janete M.Maciel,Rui M. B.Dias-da-Silva,Magnus R. DNA RNA nucleic acid extraction FNA FFPE tissue blood OBJECTIVE: Adequate isolation of nucleic acids from peripheral blood, fine-needle aspiration cells in stained slides, and fresh and formalin-fixed/paraffin-embedded tissues is crucial to ensure the success of molecular endocrinology techniques, especially when samples are stored for long periods, or when no other samples can be collected from patients who are lost to follow-up. Here, we evaluate several procedures to improve current methodologies for DNA (salting-out) and RNA isolation. MATERIALS AND METHODS: We used proteinase K treatment, heat shock, and other adaptations to increase the amount and quality of the material retrieved from the samples. RESULTS: We successfully isolated DNA and RNA from the samples described above, and this material was suitable for PCR, methylation profiling, real-time PCR and DNA sequencing. CONCLUSION: The techniques herein applied to isolate nucleic acids allowed further reliable molecular analyses. Arq Bras Endocrinol Metab. 2012;56(9):618-26info:eu-repo/semantics/openAccessSociedade Brasileira de Endocrinologia e MetabologiaArquivos Brasileiros de Endocrinologia & Metabologia v.56 n.9 20122012-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27302012000900004en10.1590/S0004-27302012000900004 |
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Kizys,Marina M. L. Cardoso,Mirian G. Lindsey,Susan C. Harada,Michelle Y. Soares,Fernando A. Melo,Maria Clara C. Montoya,Marlyn Z. Kasamatsu,Teresa S. Kunii,Ilda S. Giannocco,Gisele Martins,João Roberto M. Cerutti,Janete M. Maciel,Rui M. B. Dias-da-Silva,Magnus R. |
spellingShingle |
Kizys,Marina M. L. Cardoso,Mirian G. Lindsey,Susan C. Harada,Michelle Y. Soares,Fernando A. Melo,Maria Clara C. Montoya,Marlyn Z. Kasamatsu,Teresa S. Kunii,Ilda S. Giannocco,Gisele Martins,João Roberto M. Cerutti,Janete M. Maciel,Rui M. B. Dias-da-Silva,Magnus R. Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues, and long-term stored blood samples |
author_facet |
Kizys,Marina M. L. Cardoso,Mirian G. Lindsey,Susan C. Harada,Michelle Y. Soares,Fernando A. Melo,Maria Clara C. Montoya,Marlyn Z. Kasamatsu,Teresa S. Kunii,Ilda S. Giannocco,Gisele Martins,João Roberto M. Cerutti,Janete M. Maciel,Rui M. B. Dias-da-Silva,Magnus R. |
author_sort |
Kizys,Marina M. L. |
title |
Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues, and long-term stored blood samples |
title_short |
Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues, and long-term stored blood samples |
title_full |
Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues, and long-term stored blood samples |
title_fullStr |
Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues, and long-term stored blood samples |
title_full_unstemmed |
Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues, and long-term stored blood samples |
title_sort |
optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues, and long-term stored blood samples |
description |
OBJECTIVE: Adequate isolation of nucleic acids from peripheral blood, fine-needle aspiration cells in stained slides, and fresh and formalin-fixed/paraffin-embedded tissues is crucial to ensure the success of molecular endocrinology techniques, especially when samples are stored for long periods, or when no other samples can be collected from patients who are lost to follow-up. Here, we evaluate several procedures to improve current methodologies for DNA (salting-out) and RNA isolation. MATERIALS AND METHODS: We used proteinase K treatment, heat shock, and other adaptations to increase the amount and quality of the material retrieved from the samples. RESULTS: We successfully isolated DNA and RNA from the samples described above, and this material was suitable for PCR, methylation profiling, real-time PCR and DNA sequencing. CONCLUSION: The techniques herein applied to isolate nucleic acids allowed further reliable molecular analyses. Arq Bras Endocrinol Metab. 2012;56(9):618-26 |
publisher |
Sociedade Brasileira de Endocrinologia e Metabologia |
publishDate |
2012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27302012000900004 |
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