Biochemical and histological characterization of tomato mutants
Biochemical responses inherent to antioxidant systems as well morphological and anatomical properties of photomorphogenic, hormonal and developmental tomato mutants were investigated. Compared to the non-mutant Micro-Tom (MT), we observed that the malondialdehyde (MDA) content was enhanced in the diageotropica (dgt) and lutescent (l) mutants, whilst the highest levels of hydrogen peroxide (H2O2) were observed in high pigment 1 (hp1) and aurea (au) mutants. The analyses of antioxidant enzymes revealed that all mutants exhibited reduced catalase (CAT) activity when compared to MT. Guaiacol peroxidase (GPOX) was enhanced in both sitiens (sit) and notabilis (not) mutants, whereas in not mutant there was an increase in ascorbate peroxidase (APX). Based on PAGE analysis, the activities of glutathione reductase (GR) isoforms III, IV, V and VI were increased in l leaves, while the activity of superoxide dismutase (SOD) isoform III was reduced in leaves of sit, epi, Never ripe (Nr) and green flesh (gf) mutants. Microscopic analyses revealed that hp1 and au showed an increase in leaf intercellular spaces, whereas sit exhibited a decrease. The au and hp1 mutants also exhibited a decreased in the number of leaf trichomes. The characterization of these mutants is essential for their future use in plant development and ecophysiology studies, such as abiotic and biotic stresses on the oxidative metabolism.
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Academia Brasileira de Ciências
2012
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200025 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S0001-37652012000200025 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S0001-376520120002000252012-05-25Biochemical and histological characterization of tomato mutantsMonteiro,Carolina C.Rolão,Milca B.Franco,Mônica R.Peters,Leila P.Cia,Mariana C.Capaldi,Flávia R.Carvalho,Rogério F.Gratão,Priscila L.Rossi,Mônica L.Martinelli,Adriana P.Peres,Lázaro E.P.Azevedo,Ricardo A. antioxidant enzymes leaf and root anatomy oxidative stress Solanum lycopersicum Biochemical responses inherent to antioxidant systems as well morphological and anatomical properties of photomorphogenic, hormonal and developmental tomato mutants were investigated. Compared to the non-mutant Micro-Tom (MT), we observed that the malondialdehyde (MDA) content was enhanced in the diageotropica (dgt) and lutescent (l) mutants, whilst the highest levels of hydrogen peroxide (H2O2) were observed in high pigment 1 (hp1) and aurea (au) mutants. The analyses of antioxidant enzymes revealed that all mutants exhibited reduced catalase (CAT) activity when compared to MT. Guaiacol peroxidase (GPOX) was enhanced in both sitiens (sit) and notabilis (not) mutants, whereas in not mutant there was an increase in ascorbate peroxidase (APX). Based on PAGE analysis, the activities of glutathione reductase (GR) isoforms III, IV, V and VI were increased in l leaves, while the activity of superoxide dismutase (SOD) isoform III was reduced in leaves of sit, epi, Never ripe (Nr) and green flesh (gf) mutants. Microscopic analyses revealed that hp1 and au showed an increase in leaf intercellular spaces, whereas sit exhibited a decrease. The au and hp1 mutants also exhibited a decreased in the number of leaf trichomes. The characterization of these mutants is essential for their future use in plant development and ecophysiology studies, such as abiotic and biotic stresses on the oxidative metabolism.info:eu-repo/semantics/openAccessAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências v.84 n.2 20122012-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200025en10.1590/S0001-37652012005000022 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Monteiro,Carolina C. Rolão,Milca B. Franco,Mônica R. Peters,Leila P. Cia,Mariana C. Capaldi,Flávia R. Carvalho,Rogério F. Gratão,Priscila L. Rossi,Mônica L. Martinelli,Adriana P. Peres,Lázaro E.P. Azevedo,Ricardo A. |
spellingShingle |
Monteiro,Carolina C. Rolão,Milca B. Franco,Mônica R. Peters,Leila P. Cia,Mariana C. Capaldi,Flávia R. Carvalho,Rogério F. Gratão,Priscila L. Rossi,Mônica L. Martinelli,Adriana P. Peres,Lázaro E.P. Azevedo,Ricardo A. Biochemical and histological characterization of tomato mutants |
author_facet |
Monteiro,Carolina C. Rolão,Milca B. Franco,Mônica R. Peters,Leila P. Cia,Mariana C. Capaldi,Flávia R. Carvalho,Rogério F. Gratão,Priscila L. Rossi,Mônica L. Martinelli,Adriana P. Peres,Lázaro E.P. Azevedo,Ricardo A. |
author_sort |
Monteiro,Carolina C. |
title |
Biochemical and histological characterization of tomato mutants |
title_short |
Biochemical and histological characterization of tomato mutants |
title_full |
Biochemical and histological characterization of tomato mutants |
title_fullStr |
Biochemical and histological characterization of tomato mutants |
title_full_unstemmed |
Biochemical and histological characterization of tomato mutants |
title_sort |
biochemical and histological characterization of tomato mutants |
description |
Biochemical responses inherent to antioxidant systems as well morphological and anatomical properties of photomorphogenic, hormonal and developmental tomato mutants were investigated. Compared to the non-mutant Micro-Tom (MT), we observed that the malondialdehyde (MDA) content was enhanced in the diageotropica (dgt) and lutescent (l) mutants, whilst the highest levels of hydrogen peroxide (H2O2) were observed in high pigment 1 (hp1) and aurea (au) mutants. The analyses of antioxidant enzymes revealed that all mutants exhibited reduced catalase (CAT) activity when compared to MT. Guaiacol peroxidase (GPOX) was enhanced in both sitiens (sit) and notabilis (not) mutants, whereas in not mutant there was an increase in ascorbate peroxidase (APX). Based on PAGE analysis, the activities of glutathione reductase (GR) isoforms III, IV, V and VI were increased in l leaves, while the activity of superoxide dismutase (SOD) isoform III was reduced in leaves of sit, epi, Never ripe (Nr) and green flesh (gf) mutants. Microscopic analyses revealed that hp1 and au showed an increase in leaf intercellular spaces, whereas sit exhibited a decrease. The au and hp1 mutants also exhibited a decreased in the number of leaf trichomes. The characterization of these mutants is essential for their future use in plant development and ecophysiology studies, such as abiotic and biotic stresses on the oxidative metabolism. |
publisher |
Academia Brasileira de Ciências |
publishDate |
2012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200025 |
work_keys_str_mv |
AT monteirocarolinac biochemicalandhistologicalcharacterizationoftomatomutants AT rolaomilcab biochemicalandhistologicalcharacterizationoftomatomutants AT francomonicar biochemicalandhistologicalcharacterizationoftomatomutants AT petersleilap biochemicalandhistologicalcharacterizationoftomatomutants AT ciamarianac biochemicalandhistologicalcharacterizationoftomatomutants AT capaldiflaviar biochemicalandhistologicalcharacterizationoftomatomutants AT carvalhorogeriof biochemicalandhistologicalcharacterizationoftomatomutants AT grataopriscilal biochemicalandhistologicalcharacterizationoftomatomutants AT rossimonical biochemicalandhistologicalcharacterizationoftomatomutants AT martinelliadrianap biochemicalandhistologicalcharacterizationoftomatomutants AT pereslazaroep biochemicalandhistologicalcharacterizationoftomatomutants AT azevedoricardoa biochemicalandhistologicalcharacterizationoftomatomutants |
_version_ |
1756371955698630656 |