Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico

Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico

A cross-sectional study was conducted with a sample of 40 dogs with clinical signs suggestive of infection by Leishmania spp and Trypanosoma cruzi in the municipality of Lázaro Cárdenas, Quintana Roo, Mexico. Blood, skin and bone marrow samples were taken. Indirect immunofluorescence (IFI) tests for detection of anti-Leishmania infantum and immunochromatic test for the detection of anti-Trypanosoma cruzi antibodies in serum were performed. In addition, molecular analyses were performed using PCR to detect Leishmania spp in bone marrow and skin and T. cruzi in bone marrow. Histopathological analyses of the skin and cytology of the bone marrow were carried out in search of lesions and trypanosomatid agents. The results showed that 3 (7.5%) and 6 (15%) dogs with anti-L. infantum and T. cruzi antibodies were detected, respectively. Molecular analyses detected 17 dogs positive for infection by T. cruzi (42.5%) and none for infection by Leishmania spp. Dogs with low body condition showed greater seropositivity to T. cruzi using Fisher’s exact test (p<0.05). Parasites of the genus Leishmania did not actively participate in the pathological processes of the animals; however, a high frequency of animals infected with T. cruzi was observed.

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Main Authors: Salazar Grosskelwing, Enrique, Bolio González, Manuel Emilio, Rodríguez Vivas, Roger Iván, Miró Corrales, Guadalupe, Escobedo Ortegón, Francisco Javier, Ruíz Piña, Hugo Antonio, Loría Cervera, Elsy Nalleli
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2020
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17336
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institution UNMSM
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country Perú
countrycode PE
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region America del Sur
libraryname Biblioteca de la Facultad de Medicina Veterinaria
language spa
format Digital
author Salazar Grosskelwing, Enrique
Bolio González, Manuel Emilio
Rodríguez Vivas, Roger Iván
Miró Corrales, Guadalupe
Escobedo Ortegón, Francisco Javier
Ruíz Piña, Hugo Antonio
Loría Cervera, Elsy Nalleli
spellingShingle Salazar Grosskelwing, Enrique
Bolio González, Manuel Emilio
Rodríguez Vivas, Roger Iván
Miró Corrales, Guadalupe
Escobedo Ortegón, Francisco Javier
Ruíz Piña, Hugo Antonio
Loría Cervera, Elsy Nalleli
Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico
author_facet Salazar Grosskelwing, Enrique
Bolio González, Manuel Emilio
Rodríguez Vivas, Roger Iván
Miró Corrales, Guadalupe
Escobedo Ortegón, Francisco Javier
Ruíz Piña, Hugo Antonio
Loría Cervera, Elsy Nalleli
author_sort Salazar Grosskelwing, Enrique
title Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico
title_short Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico
title_full Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico
title_fullStr Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico
title_full_unstemmed Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico
title_sort serological and molecular frequency of leishmania spp and trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in quintana roo, mexico
description A cross-sectional study was conducted with a sample of 40 dogs with clinical signs suggestive of infection by Leishmania spp and Trypanosoma cruzi in the municipality of Lázaro Cárdenas, Quintana Roo, Mexico. Blood, skin and bone marrow samples were taken. Indirect immunofluorescence (IFI) tests for detection of anti-Leishmania infantum and immunochromatic test for the detection of anti-Trypanosoma cruzi antibodies in serum were performed. In addition, molecular analyses were performed using PCR to detect Leishmania spp in bone marrow and skin and T. cruzi in bone marrow. Histopathological analyses of the skin and cytology of the bone marrow were carried out in search of lesions and trypanosomatid agents. The results showed that 3 (7.5%) and 6 (15%) dogs with anti-L. infantum and T. cruzi antibodies were detected, respectively. Molecular analyses detected 17 dogs positive for infection by T. cruzi (42.5%) and none for infection by Leishmania spp. Dogs with low body condition showed greater seropositivity to T. cruzi using Fisher’s exact test (p<0.05). Parasites of the genus Leishmania did not actively participate in the pathological processes of the animals; however, a high frequency of animals infected with T. cruzi was observed.
publisher Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publishDate 2020
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17336
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spelling oai:ojs.csi.unmsm:article-173362020-11-27T04:08:49Z Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico Frecuencia serológica y molecular de Leishmania spp y Trypanosoma cruzi y factores asociados en perros con signos sugestivos de infección por tripanosomátidos en Quintana Roo, México Salazar Grosskelwing, Enrique Bolio González, Manuel Emilio Rodríguez Vivas, Roger Iván Miró Corrales, Guadalupe Escobedo Ortegón, Francisco Javier Ruíz Piña, Hugo Antonio Loría Cervera, Elsy Nalleli dogs indirect immunofluorescence Leishmania spp PCR trypanosomatids Trypanosoma cruzi inmunofluorescencia indirecta Leishmania spp PCR perros tripanosomátidos Trypanosoma cruzi A cross-sectional study was conducted with a sample of 40 dogs with clinical signs suggestive of infection by Leishmania spp and Trypanosoma cruzi in the municipality of Lázaro Cárdenas, Quintana Roo, Mexico. Blood, skin and bone marrow samples were taken. Indirect immunofluorescence (IFI) tests for detection of anti-Leishmania infantum and immunochromatic test for the detection of anti-Trypanosoma cruzi antibodies in serum were performed. In addition, molecular analyses were performed using PCR to detect Leishmania spp in bone marrow and skin and T. cruzi in bone marrow. Histopathological analyses of the skin and cytology of the bone marrow were carried out in search of lesions and trypanosomatid agents. The results showed that 3 (7.5%) and 6 (15%) dogs with anti-L. infantum and T. cruzi antibodies were detected, respectively. Molecular analyses detected 17 dogs positive for infection by T. cruzi (42.5%) and none for infection by Leishmania spp. Dogs with low body condition showed greater seropositivity to T. cruzi using Fisher’s exact test (p<0.05). Parasites of the genus Leishmania did not actively participate in the pathological processes of the animals; however, a high frequency of animals infected with T. cruzi was observed. Se realizó un estudio transversal con una muestra de 40 perros con signos clínicos sugestivos de infección por Leishmania spp y Trypanosoma cruzi en el municipio de Lázaro Cárdenas, Quintana Roo, México. Se tomaron muestras de sangre, piel y médula ósea. Se realizaron las pruebas de inmunofluorescencia indirecta (IFI) para detección de anticuerpos anti-Leishmania infantum e inmunocromática para detección de anticuerpos anti-Trypanosoma cruzi en suero. Además, se realizaron análisis moleculares, mediante PCR para la detección de Leishmania spp en médula ósea y piel y T. cruzi en médula ósea. Se realizaron análisis histopatológicos de piel y citológicos de médula ósea en busca de lesiones y agentes tripanosomátidos. Se detectaron 3 (7.5%) y 6 (15%) perros con anticuerpos anti-L. infantum y T. cruzi, respectivamente. En los análisis moleculares se detectaron 17 perros positivos a la infección por T. cruzi (42.5%) y ninguno a la infección por Leishmania spp. Los perros con baja condición corporal presentaron mayor seropositividad a T. cruzi mediante la prueba exacta de Fisher (p<0.05). Los parásitos del género Leishmania no participaron activamente en los procesos patológicos de los animales estudiados; sin embargo, se observa una alta frecuencia de animales infectados por T. cruzi. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2020-11-24 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17336 10.15381/rivep.v31i4.17336 Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 4 (2020); e17336 Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 4 (2020); e17336 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17336/16048 Derechos de autor 2020 Enrique Salazar Grosskelwing, Manuel Emilio Bolio González, Roger Iván Rodríguez Vivas, Guadalupe Miró Corrales, Francisco Javier Escobedo Ortegón, Hugo Antonio Ruíz Piña, Elsy Nalleli Loría Cervera https://creativecommons.org/licenses/by-nc-sa/4.0

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