In vitro evaluation of the nematicide activity of the liquid fermentation of Purpureocillium sp. UdeA 0106 strain on pineapple and flower soils

In vitro evaluation of the nematicide activity of the liquid fermentation of Purpureocillium sp. UdeA 0106 strain on pineapple and flower soils

Colombia is recognized as an important agricultural producer of pineapple and flowers. One of the most significant phytosanitary problems of these crops is pathogenic nematodes and it is necessary to find biocontrol alternatives for their management. This study evaluated the effect of Purpureocillium sp., strain UdeA0106, over the number of nematodes. Soil from pineapple and flower crops was collected on expanded polystyrene containers and suspension of the fungi was multiplied in a bioreactor (1x108 conidia/mL), then homogenized and finally applied. The control consisted of the containers with soil and sterile distilled water (SDW) and 4 to 6 repetitions were evaluated on each crop. The number of individuals of each genus on 100g of soil was analyzed using R studio®, Kruskal-Wallis, and van der Waals tests were applied to evaluate significant differences between strain and control (P=0.05). The results showed that the strain did not affect the saprophytic nematodes but decreased the number of Meloidogyne spp., Paratylenchus spp., Helicotylenchus spp., and Tylenchus spp; no biocontrollers had been reported before for the last two genera.

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Main Authors: Cardona, Nadya Lorena, Hernández, Dayana Michel, Hoyos, Laura A., Medina, Juan Diego
Format: Digital revista
Language:eng
Published: Coeditada entre Facultad de Agronomía - Udelar y el Instituto Nacional de Investigación Agropecuaria (INIA) 2020
Online Access:https://agrocienciauruguay.uy/index.php/agrociencia/article/view/382
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record_format ojs
institution UDELAR
collection OJS
country Uruguay
countrycode UY
component Revista
access En linea
databasecode rev-agrociencia-uy
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region America del Sur
libraryname Biblioteca de la Facultad de Agronomía de la UDELAR de UY
language eng
format Digital
author Cardona, Nadya Lorena
Hernández, Dayana Michel
Hoyos, Laura A.
Medina, Juan Diego
spellingShingle Cardona, Nadya Lorena
Hernández, Dayana Michel
Hoyos, Laura A.
Medina, Juan Diego
In vitro evaluation of the nematicide activity of the liquid fermentation of Purpureocillium sp. UdeA 0106 strain on pineapple and flower soils
author_facet Cardona, Nadya Lorena
Hernández, Dayana Michel
Hoyos, Laura A.
Medina, Juan Diego
author_sort Cardona, Nadya Lorena
title In vitro evaluation of the nematicide activity of the liquid fermentation of Purpureocillium sp. UdeA 0106 strain on pineapple and flower soils
title_short In vitro evaluation of the nematicide activity of the liquid fermentation of Purpureocillium sp. UdeA 0106 strain on pineapple and flower soils
title_full In vitro evaluation of the nematicide activity of the liquid fermentation of Purpureocillium sp. UdeA 0106 strain on pineapple and flower soils
title_fullStr In vitro evaluation of the nematicide activity of the liquid fermentation of Purpureocillium sp. UdeA 0106 strain on pineapple and flower soils
title_full_unstemmed In vitro evaluation of the nematicide activity of the liquid fermentation of Purpureocillium sp. UdeA 0106 strain on pineapple and flower soils
title_sort in vitro evaluation of the nematicide activity of the liquid fermentation of purpureocillium sp. udea 0106 strain on pineapple and flower soils
description Colombia is recognized as an important agricultural producer of pineapple and flowers. One of the most significant phytosanitary problems of these crops is pathogenic nematodes and it is necessary to find biocontrol alternatives for their management. This study evaluated the effect of Purpureocillium sp., strain UdeA0106, over the number of nematodes. Soil from pineapple and flower crops was collected on expanded polystyrene containers and suspension of the fungi was multiplied in a bioreactor (1x108 conidia/mL), then homogenized and finally applied. The control consisted of the containers with soil and sterile distilled water (SDW) and 4 to 6 repetitions were evaluated on each crop. The number of individuals of each genus on 100g of soil was analyzed using R studio®, Kruskal-Wallis, and van der Waals tests were applied to evaluate significant differences between strain and control (P=0.05). The results showed that the strain did not affect the saprophytic nematodes but decreased the number of Meloidogyne spp., Paratylenchus spp., Helicotylenchus spp., and Tylenchus spp; no biocontrollers had been reported before for the last two genera.
publisher Coeditada entre Facultad de Agronomía - Udelar y el Instituto Nacional de Investigación Agropecuaria (INIA)
publishDate 2020
url https://agrocienciauruguay.uy/index.php/agrociencia/article/view/382
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spelling oai:oai.agrocienciauruguay.uy:article-3822021-12-22T23:59:43Z In vitro evaluation of the nematicide activity of the liquid fermentation of Purpureocillium sp. UdeA 0106 strain on pineapple and flower soils Evaluación in vitro de la actividad nematicida de la fermentación líquida de Purpureocillium sp. cepa UdeA 0106 en suelos de piña y flores Avaliação in vitro da actividade nematicida da fermentação líquida da estirpe de Purpureocillium sp. UdeA 0106 em solos de ananás e flores Cardona, Nadya Lorena Hernández, Dayana Michel Hoyos, Laura A. Medina, Juan Diego biocontrol biocontroller fungi Purpureocillium spp. pineapple flowers biocontrol hongos biocontroladores Purpureocillium sp. piña flores biocontrol fungos biocontrol Purpureocillium sp. abacaxi flores Colombia is recognized as an important agricultural producer of pineapple and flowers. One of the most significant phytosanitary problems of these crops is pathogenic nematodes and it is necessary to find biocontrol alternatives for their management. This study evaluated the effect of Purpureocillium sp., strain UdeA0106, over the number of nematodes. Soil from pineapple and flower crops was collected on expanded polystyrene containers and suspension of the fungi was multiplied in a bioreactor (1x108 conidia/mL), then homogenized and finally applied. The control consisted of the containers with soil and sterile distilled water (SDW) and 4 to 6 repetitions were evaluated on each crop. The number of individuals of each genus on 100g of soil was analyzed using R studio®, Kruskal-Wallis, and van der Waals tests were applied to evaluate significant differences between strain and control (P=0.05). The results showed that the strain did not affect the saprophytic nematodes but decreased the number of Meloidogyne spp., Paratylenchus spp., Helicotylenchus spp., and Tylenchus spp; no biocontrollers had been reported before for the last two genera. Colombia es reconocida como un importante productor agrícola de piña y flores. Uno de los problemas fitosanitarios más importantes de estos cultivos son los nematodos patógenos y es necesario encontrar alternativas de biocontrol para su manejo. Este estudio evaluó el efecto de Purpureocillium sp., Cepa UdeA0106 sobre poblaciones de nematodos fitopatógenos. Suelos de cultivos de piña y flores se colectaron en contenedores de poliestireno expandido y la suspensión del hongo se multiplicó en un biorreactor (1x108conidias/mL), se homogeneizó y luego se aplicó; para los controles se utilizó agua destilada esterilizada (DEW) y se evaluaron 4 a 6 repeticiones para cada cultivo. Se evalúo el número de individuos de cada género en 100 g de suelo, y mediante R studio®, se aplicaron las pruebas de Kruskal-Wallis y van der Waals para evaluar diferencias significativas entre la cepa y el control (P = 0,05). Los resultados mostraron que la cepa no afectó a los nematodos saprófitos, pero disminuyó el número de Meloidogyne spp., Paratylenchus spp., Helicotylenchus spp. y Tylenchus spp. Para estos últimos géneros, no se habían reportado hongos biocontroladores. A Colômbia é reconhecida como um importante produtor agrícola de ananás e flores. Um dos problemas fitossanitários mais importantes destas culturas são os nematódeos patogénicos e é necessário encontrar alternativas de biocontrolo para a sua gestão. Este estudo avaliou o efeito do Purpureocillium sp., Cepa UdeA0106 nas populações de nematódeos fitopatogénicos. Os solos das culturas de ananás e flores foram recolhidos em recipientes de poliestireno expandido e a suspensão do fungo foi multiplicada num bioreactor (1x108conidia / mL), homogeneizado e depois aplicado; água destilada estéril (DEW) foi utilizada para os controlos e 4 a 6 réplicas foram avaliadas para cada cultura. O número de indivíduos de cada género em 100 g de solo foi avaliado, e utilizando os testes R study®, Kruskal-Wallis e van der Waals foram aplicados para avaliar diferenças significativas entre a estirpe e o controlo (P = 0,05). Os resultados mostraram que a estirpe não afectou os nemátodos saprófitos, mas diminuiu o número de Meloidogyne spp., Paratylenchus spp., Helicotylenchus spp. e Tylenchus spp. Para estes últimos géneros, não tinha sido relatado nenhum fungo biocontrol. Coeditada entre Facultad de Agronomía - Udelar y el Instituto Nacional de Investigación Agropecuaria (INIA) 2020-12-17 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf application/zip application/zip https://agrocienciauruguay.uy/index.php/agrociencia/article/view/382 10.31285/AGRO.24.382 Agrociencia Uruguay; Vol. 24 No. NE 2 (2020); e382 Agrociencia Uruguay; Vol. 24 Núm. NE 2 (2020); e382 Agrociencia Uruguay; v. 24 n. NE 2 (2020); e382 2730-5066 eng https://agrocienciauruguay.uy/index.php/agrociencia/article/view/382/417 https://agrocienciauruguay.uy/index.php/agrociencia/article/view/382/493 https://agrocienciauruguay.uy/index.php/agrociencia/article/view/382/494 https://agrocienciauruguay.uy/index.php/agrociencia/article/view/382/495 Copyright (c) 2020 Agrociencia Uruguay

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