Effect of a multispecies fungal additive on rumen fermentation profile, degradability and kinetic gas production
Two experiments evaluated the effect of a multispecies fungal complex (BP, BIOPREMIX MX®, Ruminal Fermentation Tech, Uruguay). In Experiment 1 (E1), the impact of adding BP to a total mixed ration (TMR) on ruminal fermentation profile and TMR in situ degradation kinetics was assessed. In Experiment 2 (E2), the effect of adding BP to various substrates on in vitro fermentability was examined. In E1, 4 Holstein cows with rumen cannulas were randomly assigned to Control (TMR with forage:concentrate ratio 75:25) or Control + 120 g/cow/d of BP (BP) and received ad libitum TMR for 30 days. Samples of TMR were ruminally incubated to estimate in situ degradation kinetic. Ruminal pH, ammonia, and volatile fatty acids (VFA) concentrations were measured just before feeding, 4 h and 8 h post feeding. In E2, a factorial arrangement included two BP levels (0 -Control or 6.5 g BP per kg dry matter incubated-WBP) and 8 substrates. In vitro gas production kinetics (GPk), dry matter digestibility (IVDMD), methanogenic potential (CH4), partitioning factor (PF), VFA, and microbial crude protein (MCP) were estimated. The BP increased proportion of propionate (P ≤ 0.05) and reduced ketogenic:glucogenic ratio and Lag phase of NDF (P ≤ 0.05). WBP tended to increase IVDMD, and substrate affected GPk, IVDMD, CH4, PF, VFA and MCP (P ≤ 0.01). Overall, BP improved ruminal metabolism favoring a more glucogenic profile, a shortening Lag phase in NDF degradation, and increasing IVDMD.
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Coeditada entre Facultad de Agronomía - Udelar y el Instituto Nacional de Investigación Agropecuaria (INIA)
2024
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Bruni, María de los Ángeles Chilibroste, Pablo Casal, Alberto Trujillo, Ana Inés |
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Bruni, María de los Ángeles Chilibroste, Pablo Casal, Alberto Trujillo, Ana Inés Effect of a multispecies fungal additive on rumen fermentation profile, degradability and kinetic gas production |
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Bruni, María de los Ángeles Chilibroste, Pablo Casal, Alberto Trujillo, Ana Inés |
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Bruni, María de los Ángeles |
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Effect of a multispecies fungal additive on rumen fermentation profile, degradability and kinetic gas production |
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Effect of a multispecies fungal additive on rumen fermentation profile, degradability and kinetic gas production |
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Effect of a multispecies fungal additive on rumen fermentation profile, degradability and kinetic gas production |
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Effect of a multispecies fungal additive on rumen fermentation profile, degradability and kinetic gas production |
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Effect of a multispecies fungal additive on rumen fermentation profile, degradability and kinetic gas production |
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effect of a multispecies fungal additive on rumen fermentation profile, degradability and kinetic gas production |
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Two experiments evaluated the effect of a multispecies fungal complex (BP, BIOPREMIX MX®, Ruminal Fermentation Tech, Uruguay). In Experiment 1 (E1), the impact of adding BP to a total mixed ration (TMR) on ruminal fermentation profile and TMR in situ degradation kinetics was assessed. In Experiment 2 (E2), the effect of adding BP to various substrates on in vitro fermentability was examined. In E1, 4 Holstein cows with rumen cannulas were randomly assigned to Control (TMR with forage:concentrate ratio 75:25) or Control + 120 g/cow/d of BP (BP) and received ad libitum TMR for 30 days. Samples of TMR were ruminally incubated to estimate in situ degradation kinetic. Ruminal pH, ammonia, and volatile fatty acids (VFA) concentrations were measured just before feeding, 4 h and 8 h post feeding. In E2, a factorial arrangement included two BP levels (0 -Control or 6.5 g BP per kg dry matter incubated-WBP) and 8 substrates. In vitro gas production kinetics (GPk), dry matter digestibility (IVDMD), methanogenic potential (CH4), partitioning factor (PF), VFA, and microbial crude protein (MCP) were estimated. The BP increased proportion of propionate (P ≤ 0.05) and reduced ketogenic:glucogenic ratio and Lag phase of NDF (P ≤ 0.05). WBP tended to increase IVDMD, and substrate affected GPk, IVDMD, CH4, PF, VFA and MCP (P ≤ 0.01). Overall, BP improved ruminal metabolism favoring a more glucogenic profile, a shortening Lag phase in NDF degradation, and increasing IVDMD. |
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Coeditada entre Facultad de Agronomía - Udelar y el Instituto Nacional de Investigación Agropecuaria (INIA) |
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2024 |
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oai:oai.agrocienciauruguay.uy:article-12142024-09-18T11:49:28Z Effect of a multispecies fungal additive on rumen fermentation profile, degradability and kinetic gas production Efecto de un aditivo fúngico multiespecie sobre perfil de fermentación ruminal, degradabilidad y cinética de producción de gas Efeito de um aditivo fúngico multiespécie no perfil de fermentação ruminal, de-gradabilidade e cinética de produção de gases Bruni, María de los Ángeles Chilibroste, Pablo Casal, Alberto Trujillo, Ana Inés multifungal additive in situ kinetic in vitro fermentability aditivo multifúngico cinética in situ fermentabilidad in vitro aditivo multifúngico cinética in situ fermentabilidade in vitro Two experiments evaluated the effect of a multispecies fungal complex (BP, BIOPREMIX MX®, Ruminal Fermentation Tech, Uruguay). In Experiment 1 (E1), the impact of adding BP to a total mixed ration (TMR) on ruminal fermentation profile and TMR in situ degradation kinetics was assessed. In Experiment 2 (E2), the effect of adding BP to various substrates on in vitro fermentability was examined. In E1, 4 Holstein cows with rumen cannulas were randomly assigned to Control (TMR with forage:concentrate ratio 75:25) or Control + 120 g/cow/d of BP (BP) and received ad libitum TMR for 30 days. Samples of TMR were ruminally incubated to estimate in situ degradation kinetic. Ruminal pH, ammonia, and volatile fatty acids (VFA) concentrations were measured just before feeding, 4 h and 8 h post feeding. In E2, a factorial arrangement included two BP levels (0 -Control or 6.5 g BP per kg dry matter incubated-WBP) and 8 substrates. In vitro gas production kinetics (GPk), dry matter digestibility (IVDMD), methanogenic potential (CH4), partitioning factor (PF), VFA, and microbial crude protein (MCP) were estimated. The BP increased proportion of propionate (P ≤ 0.05) and reduced ketogenic:glucogenic ratio and Lag phase of NDF (P ≤ 0.05). WBP tended to increase IVDMD, and substrate affected GPk, IVDMD, CH4, PF, VFA and MCP (P ≤ 0.01). Overall, BP improved ruminal metabolism favoring a more glucogenic profile, a shortening Lag phase in NDF degradation, and increasing IVDMD. Dos experimentos evaluaron el efecto de un complejo fúngico multiespecies (BP, BIOPREMIX MX®, Ruminal Fermentation Tech, Uruguay). En el Experimento 1 (E1) se evaluó el impacto de agregar BP a una ración totalmente mezclada (TMR) sobre la fermentación ruminal y la cinética de degradación de la TMR. En el Experimento 2 (E2) se evaluó el efecto de agregar BP a varios sustratos sobre la fermentabilidad in vitro. En E1, 4 vacas Holstein con cánulas ruminales fueron asignadas aleatoriamente a Control (TMR con relación forraje:concentrado 75:25) o Control + 120 g/vaca/d de BP (BP), y recibieron TMR ad libitum durante 30 días. Se incubaron ruminalmente muestras de TMR para estimar la cinética de degradación in situ. Se midió pH, concentración de amoníaco y de ácidos grasos volátiles (VFA) en líquido ruminal antes de la alimentación, 4 y 8 h después de la misma. En E2, un arreglo factorial incluyó dos niveles de BP (0 -Control o 6.5 g BP por kg de materia seca incubada -WBP) y 8 sustratos. Se estimaron la cinética de producción de gas in vitro (GPk), la digestibilidad de la materia seca (IVDMD), el potencial metanogénico (CH4), el factor de partición (PF), VFA y la proteína microbiana (MCP). El BP aumentó la proporción de propionato (P ≤ 0,05) y redujo la relación cetogénica:glucogénica y la fase Lag de la FDN (P ≤ 0,05). WBP tendió a aumentar la IVDMD, y el sustrato afectó a GPk, IVDMD, CH4, PF, VFA y MCP (P ≤ 0,01). En general, BP mejoró el metabolismo ruminal favoreciendo un perfil más glucogénico, una fase Lag más corta en la degradación de FDN y un aumento de la IVDMD. Dois experimentos avaliaramo efeito de um complexo fúngico multiespécie (BP, BIOPREMIX MX®, Ruminal Fermentation Tech, Uruguai). No Experimento 1 (E1) avaliou o efeito da adição de BP a uma ração mista total (TMR) no perfil de fermentação ruminal e na cinética de degradação in situ da TMR. No Experimento 2 (E2) avaliou o efeito da adição de BP a diferentes substratos na fermentabilidade in vitro. No E1, 4 vacas Holstein com cânulas ruminais foram aleatoriamente designadas para Controle (TMR com relação volumoso:concentrado 75:25) ou Controle + 120 g/vaca/d de BP (BP) e receberam TMR ad libitum por 30 dias. As mostras de TMR foram incubadas no rúmen para estimar a cinética de degradação in situ. O pH e concentrações de amônia e ácidos graxos voláteis (VFA) foram medidas no líquido ruminal imediatamente antes da alimentação, 4 horas e 8 horas após a alimentação. Em E2, um arranjo fatorial de níveis de BP (6,5 g BP/ kg MS incubado –WBP ou sem BP -Control) e 8 substratos. Foram estimadas a cinética de produção de gases in vitro (GPk), a digestibilidade da matéria seca (IVDMD), o potencial metanogênico (CH4), o fator de partição (FP), os AGV e a proteína microbiana (MCP). O BP aumentou a proporção de propionato (P ≤ 0,05) e reduziu a relação cetogênico:glicogênico e a fase Lag da FDN (P ≤ 0,05). O WBP tendeuaaumentara IVDMD e o substrato afetou, IVDMD, CH4, PF, VFA total e MCP (P ≤ 0,01). No geral, BP melhorou o metabolismo ruminal favorecendo um perfil mais glicogênico, um encurtamento da fase Lag na degradação da FDN e um aumento na IVDMD. Coeditada entre Facultad de Agronomía - Udelar y el Instituto Nacional de Investigación Agropecuaria (INIA) 2024-05-23 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://agrocienciauruguay.uy/index.php/agrociencia/article/view/1214 10.31285/AGRO.28.1214 Agrociencia Uruguay; Vol. 28 No. NE1 (2024); e1214 Agrociencia Uruguay; Vol. 28 Núm. 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Doi: 10.3168/jds.S0022-0302(99)75245-8. Copyright (c) 2024 Agrociencia Uruguay https://creativecommons.org/licenses/by/4.0 |