Determination of imidacloprid in beehive samples by UHPLC-MS/MS
Imidacloprid is a systemic insecticide belonging to the neonicotinoid family. It was the first neonicotinoid introduced in the mid-1990s, and since then, its use has grown rapidly to control pests in a variety of agricultural crops. Several studies have shown that neonicotinoids translocate to the nectar and pollen of treated plants, which represents a potential risk to pollinators. Therefore, an open-field feeding study was carried out. For this purpose, 30 beehives of Apis mellifera L. were installed in the same apiary. All colonies were in similar health and population conditions when assays were started. For seven weeks, colonies were fed with sucrose syrup with different concentrations of imidacloprid: 15, 30, 120 and 240 μg kg−1. Thus, the assays were divided into four treatments and a witness (Control) with no added imidacloprid. To check the hives' exposure to imidacloprid and evaluate its distribution, sampling of adult worker bees and larvae was performed before, during and after the whole feeding period (7 weeks). Furthermore, in the 15th week, honey and beeswax (honeycomb) samples were collected from the brood chamber and honey super of all hives. Analytical methodologies for sample preparation based on the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) procedure were optimized and validated. After soaking the bees and honey samples and the extraction using acetonitrile with MgSO4 and NaCl salts, a dispersive solid-phase extraction (d-SPE) step with MgSO4, PSA and C18 was applied. Melted beeswax was subjected to an acetonitrile extraction, followed by freeze-out and d-SPE with PSA and C18. Extracts were evaluated in a UHPLC-MS/MS system. LOQ (μg kg−1) values were 0.25, 0.50 and 1 for honey, bees and beeswax, respectively. Satisfactory recovery performance was achieved with relative standard deviation ≤20%. Residue concentrations of imidacloprid in samples showed correlation with the doses supplied, indicating exposure of the beehives to the insecticide. Honey store
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2018
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Subjects: | Imidacloprid, Colmena, Insecticidas, Abeja Melífera, Métodos, Hives, Insecticides, Honey Bees, Methods, |
Online Access: | https://www.sciencedirect.com/science/article/pii/S0026265X18303278 http://hdl.handle.net/20.500.12123/3002 https://doi.org/10.1016/j.microc.2018.07.027 |
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oai:localhost:20.500.12123-30022018-08-07T14:14:34Z Determination of imidacloprid in beehive samples by UHPLC-MS/MS Michlig, Melina P. Merke, Julieta Pacini, Adriana Cecilia Orellano, Emanuel Beldoménico, Horacio Ramón Repetti, María Rosa Imidacloprid Colmena Insecticidas Abeja Melífera Métodos Hives Insecticides Honey Bees Methods Imidacloprid is a systemic insecticide belonging to the neonicotinoid family. It was the first neonicotinoid introduced in the mid-1990s, and since then, its use has grown rapidly to control pests in a variety of agricultural crops. Several studies have shown that neonicotinoids translocate to the nectar and pollen of treated plants, which represents a potential risk to pollinators. Therefore, an open-field feeding study was carried out. For this purpose, 30 beehives of Apis mellifera L. were installed in the same apiary. All colonies were in similar health and population conditions when assays were started. For seven weeks, colonies were fed with sucrose syrup with different concentrations of imidacloprid: 15, 30, 120 and 240 μg kg−1. Thus, the assays were divided into four treatments and a witness (Control) with no added imidacloprid. To check the hives' exposure to imidacloprid and evaluate its distribution, sampling of adult worker bees and larvae was performed before, during and after the whole feeding period (7 weeks). Furthermore, in the 15th week, honey and beeswax (honeycomb) samples were collected from the brood chamber and honey super of all hives. Analytical methodologies for sample preparation based on the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) procedure were optimized and validated. After soaking the bees and honey samples and the extraction using acetonitrile with MgSO4 and NaCl salts, a dispersive solid-phase extraction (d-SPE) step with MgSO4, PSA and C18 was applied. Melted beeswax was subjected to an acetonitrile extraction, followed by freeze-out and d-SPE with PSA and C18. Extracts were evaluated in a UHPLC-MS/MS system. LOQ (μg kg−1) values were 0.25, 0.50 and 1 for honey, bees and beeswax, respectively. Satisfactory recovery performance was achieved with relative standard deviation ≤20%. Residue concentrations of imidacloprid in samples showed correlation with the doses supplied, indicating exposure of the beehives to the insecticide. Honey store EEA Rafaela Fil: Michlig, Melina P. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Merke, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Pacini, Adriana Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Orellano, Emanuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Beldoménico, Horacio Ramón. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina Fil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina 2018-08-07T14:11:34Z 2018-08-07T14:11:34Z 2018 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion https://www.sciencedirect.com/science/article/pii/S0026265X18303278 http://hdl.handle.net/20.500.12123/3002 0026-265X https://doi.org/10.1016/j.microc.2018.07.027 eng info:eu-repo/semantics/embargoedAccess application/pdf Microchemical Journal 143 : 72-81 (2018) |
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Imidacloprid Colmena Insecticidas Abeja Melífera Métodos Hives Insecticides Honey Bees Methods Imidacloprid Colmena Insecticidas Abeja Melífera Métodos Hives Insecticides Honey Bees Methods |
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Imidacloprid Colmena Insecticidas Abeja Melífera Métodos Hives Insecticides Honey Bees Methods Imidacloprid Colmena Insecticidas Abeja Melífera Métodos Hives Insecticides Honey Bees Methods Michlig, Melina P. Merke, Julieta Pacini, Adriana Cecilia Orellano, Emanuel Beldoménico, Horacio Ramón Repetti, María Rosa Determination of imidacloprid in beehive samples by UHPLC-MS/MS |
description |
Imidacloprid is a systemic insecticide belonging to the neonicotinoid family. It was the first neonicotinoid introduced in the mid-1990s, and since then, its use has grown rapidly to control pests in a variety of agricultural crops. Several studies have shown that neonicotinoids translocate to the nectar and pollen of treated plants, which represents a potential risk to pollinators. Therefore, an open-field feeding study was carried out. For this purpose, 30 beehives of Apis mellifera L. were installed in the same apiary. All colonies were in similar health and population conditions when assays were started. For seven weeks, colonies were fed with sucrose syrup with different concentrations of imidacloprid: 15, 30, 120 and 240 μg kg−1. Thus, the assays were divided into four treatments and a witness (Control) with no added imidacloprid. To check the hives' exposure to imidacloprid and evaluate its distribution, sampling of adult worker bees and larvae was performed before, during and after the whole feeding period (7 weeks). Furthermore, in the 15th week, honey and beeswax (honeycomb) samples were collected from the brood chamber and honey super of all hives. Analytical methodologies for sample preparation based on the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) procedure were optimized and validated. After soaking the bees and honey samples and the extraction using acetonitrile with MgSO4 and NaCl salts, a dispersive solid-phase extraction (d-SPE) step with MgSO4, PSA and C18 was applied. Melted beeswax was subjected to an acetonitrile extraction, followed by freeze-out and d-SPE with PSA and C18. Extracts were evaluated in a UHPLC-MS/MS system. LOQ (μg kg−1) values were 0.25, 0.50 and 1 for honey, bees and beeswax, respectively. Satisfactory recovery performance was achieved with relative standard deviation ≤20%. Residue concentrations of imidacloprid in samples showed correlation with the doses supplied, indicating exposure of the beehives to the insecticide. Honey store |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Imidacloprid Colmena Insecticidas Abeja Melífera Métodos Hives Insecticides Honey Bees Methods |
author |
Michlig, Melina P. Merke, Julieta Pacini, Adriana Cecilia Orellano, Emanuel Beldoménico, Horacio Ramón Repetti, María Rosa |
author_facet |
Michlig, Melina P. Merke, Julieta Pacini, Adriana Cecilia Orellano, Emanuel Beldoménico, Horacio Ramón Repetti, María Rosa |
author_sort |
Michlig, Melina P. |
title |
Determination of imidacloprid in beehive samples by UHPLC-MS/MS |
title_short |
Determination of imidacloprid in beehive samples by UHPLC-MS/MS |
title_full |
Determination of imidacloprid in beehive samples by UHPLC-MS/MS |
title_fullStr |
Determination of imidacloprid in beehive samples by UHPLC-MS/MS |
title_full_unstemmed |
Determination of imidacloprid in beehive samples by UHPLC-MS/MS |
title_sort |
determination of imidacloprid in beehive samples by uhplc-ms/ms |
publishDate |
2018 |
url |
https://www.sciencedirect.com/science/article/pii/S0026265X18303278 http://hdl.handle.net/20.500.12123/3002 https://doi.org/10.1016/j.microc.2018.07.027 |
work_keys_str_mv |
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_version_ |
1756007200941146113 |