Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers
“Smart” nanogels are an attractive tool for the development of new strategies of immunization in veterinary medicine. Here, we reported the synthesis and physicochemical characterization of thermoresponsive nanogels based on poly(N-isopropylacrylamide) (pNIPAM) and their in vitro, ex vivo and in vivo (mice model) performance. Smart nanogels of ca. 250 nm, with a transition temperature of 32 °C were obtained by precipitation polymerization. Assays to evaluate pNIPAM nanogels cytotoxicity were performed in different cell lines showing high biocompatibility (>70 %). The efficient internalization of the system was studied by confocal microscopy as well as flow cytometry. The ability to protect and deliver antigens was analyzed using the outer membrane lipoprotein A (OmlA), an important virulence factor of Actinobacillus pleuropneumoniae (App) cause of porcine pleuropneumonia. This lipoprotein was synthesized by recombinant technology and its technique was also described. The biodistribution of pNIPAM nanogels administered intranasally was performed in vivo and ex vivo through Pearl Imaging System, which showed that nanogels were kept mostly in the lungs during the evaluated time. Besides, the efficacy of the proposal nanogel-based vaccine was studied in vivo by measuring the antibody titers of BALB/c mice inoculated with OmlA encapsulated into pNIPAM nanogels compared to OmlA plus aluminum hydroxide adjuvant. The results proved the ability of nanogels to stimulate a humoral immune response. Therefore, we have demonstrated that pNIPAM nanogels can be used as an efficient platform for vaccine nanocarriers.
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Elsevier
2022-12-19T17:28:17Z
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Subjects: | Nanotecnología, Nanomateriales, Vacuna, Macrofagos, Nanotechnology, Nanomaterials, Vaccines, Macrophages, Nanogeles, Nanogels, |
Online Access: | http://hdl.handle.net/20.500.12123/13655 https://www.sciencedirect.com/science/article/abs/pii/S0378517322009905 https://doi.org/10.1016/j.ijpharm.2022.122435 |
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Nanotecnología Nanomateriales Vacuna Macrofagos Nanotechnology Nanomaterials Vaccines Macrophages Nanogeles Nanogels Nanotecnología Nanomateriales Vacuna Macrofagos Nanotechnology Nanomaterials Vaccines Macrophages Nanogeles Nanogels |
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Nanotecnología Nanomateriales Vacuna Macrofagos Nanotechnology Nanomaterials Vaccines Macrophages Nanogeles Nanogels Nanotecnología Nanomateriales Vacuna Macrofagos Nanotechnology Nanomaterials Vaccines Macrophages Nanogeles Nanogels Soriano Perez, María Laura Funes, Javier Alejandro Flores Bracamonte, Carolina Ibarra, Luis Exequiel Forrellad, Marina Andrea Taboga, Oscar Alberto Cariddi, Laura Noelia Salinas, Facundo José Ortega, Hugo Héctor Alustiza, Fabrisio Eduardo Molina, Maria Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers |
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“Smart” nanogels are an attractive tool for the development of new strategies of immunization in veterinary medicine. Here, we reported the synthesis and physicochemical characterization of thermoresponsive nanogels based on poly(N-isopropylacrylamide) (pNIPAM) and their in vitro, ex vivo and in vivo (mice model) performance. Smart nanogels of ca. 250 nm, with a transition temperature of 32 °C were obtained by precipitation polymerization. Assays to evaluate pNIPAM nanogels cytotoxicity were performed in different cell lines showing high biocompatibility (>70 %). The efficient internalization of the system was studied by confocal microscopy as well as flow cytometry. The ability to protect and deliver antigens was analyzed using the outer membrane lipoprotein A (OmlA), an important virulence factor of Actinobacillus pleuropneumoniae (App) cause of porcine pleuropneumonia. This lipoprotein was synthesized by recombinant technology and its technique was also described. The biodistribution of pNIPAM nanogels administered intranasally was performed in vivo and ex vivo through Pearl Imaging System, which showed that nanogels were kept mostly in the lungs during the evaluated time. Besides, the efficacy of the proposal nanogel-based vaccine was studied in vivo by measuring the antibody titers of BALB/c mice inoculated with OmlA encapsulated into pNIPAM nanogels compared to OmlA plus aluminum hydroxide adjuvant. The results proved the ability of nanogels to stimulate a humoral immune response. Therefore, we have demonstrated that pNIPAM nanogels can be used as an efficient platform for vaccine nanocarriers. |
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Nanotecnología Nanomateriales Vacuna Macrofagos Nanotechnology Nanomaterials Vaccines Macrophages Nanogeles Nanogels |
author |
Soriano Perez, María Laura Funes, Javier Alejandro Flores Bracamonte, Carolina Ibarra, Luis Exequiel Forrellad, Marina Andrea Taboga, Oscar Alberto Cariddi, Laura Noelia Salinas, Facundo José Ortega, Hugo Héctor Alustiza, Fabrisio Eduardo Molina, Maria |
author_facet |
Soriano Perez, María Laura Funes, Javier Alejandro Flores Bracamonte, Carolina Ibarra, Luis Exequiel Forrellad, Marina Andrea Taboga, Oscar Alberto Cariddi, Laura Noelia Salinas, Facundo José Ortega, Hugo Héctor Alustiza, Fabrisio Eduardo Molina, Maria |
author_sort |
Soriano Perez, María Laura |
title |
Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers |
title_short |
Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers |
title_full |
Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers |
title_fullStr |
Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers |
title_full_unstemmed |
Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers |
title_sort |
development and biological evaluation of pnipam-based nanogels as vaccine carriers |
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Elsevier |
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2022-12-19T17:28:17Z |
url |
http://hdl.handle.net/20.500.12123/13655 https://www.sciencedirect.com/science/article/abs/pii/S0378517322009905 https://doi.org/10.1016/j.ijpharm.2022.122435 |
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oai:localhost:20.500.12123-136552022-12-19T17:35:01Z Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers Soriano Perez, María Laura Funes, Javier Alejandro Flores Bracamonte, Carolina Ibarra, Luis Exequiel Forrellad, Marina Andrea Taboga, Oscar Alberto Cariddi, Laura Noelia Salinas, Facundo José Ortega, Hugo Héctor Alustiza, Fabrisio Eduardo Molina, Maria Nanotecnología Nanomateriales Vacuna Macrofagos Nanotechnology Nanomaterials Vaccines Macrophages Nanogeles Nanogels “Smart” nanogels are an attractive tool for the development of new strategies of immunization in veterinary medicine. Here, we reported the synthesis and physicochemical characterization of thermoresponsive nanogels based on poly(N-isopropylacrylamide) (pNIPAM) and their in vitro, ex vivo and in vivo (mice model) performance. Smart nanogels of ca. 250 nm, with a transition temperature of 32 °C were obtained by precipitation polymerization. Assays to evaluate pNIPAM nanogels cytotoxicity were performed in different cell lines showing high biocompatibility (>70 %). The efficient internalization of the system was studied by confocal microscopy as well as flow cytometry. The ability to protect and deliver antigens was analyzed using the outer membrane lipoprotein A (OmlA), an important virulence factor of Actinobacillus pleuropneumoniae (App) cause of porcine pleuropneumonia. This lipoprotein was synthesized by recombinant technology and its technique was also described. The biodistribution of pNIPAM nanogels administered intranasally was performed in vivo and ex vivo through Pearl Imaging System, which showed that nanogels were kept mostly in the lungs during the evaluated time. Besides, the efficacy of the proposal nanogel-based vaccine was studied in vivo by measuring the antibody titers of BALB/c mice inoculated with OmlA encapsulated into pNIPAM nanogels compared to OmlA plus aluminum hydroxide adjuvant. The results proved the ability of nanogels to stimulate a humoral immune response. Therefore, we have demonstrated that pNIPAM nanogels can be used as an efficient platform for vaccine nanocarriers. EEA Marcos Juárez Fil: Soriano Perez, María Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Funes, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Flores Bracamonte, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina Fil: Flores Bracamonte, Carolina. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; Argentina Fil: Ibarra, Luis Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina Fil: Ibarra, Luis Exequiel. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina Fil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Forrellad, Marina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Taboga, Oscar Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cariddi, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina Fil: Salinas, Facundo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Salinas, Facundo José. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Ortega, Hugo Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Ortega, Hugo Héctor. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Molina, Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Molina, Maria. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina info:eu-repo/date/embargoEnd/2023-12-19 2022-12-19T17:28:17Z 2022-12-19T17:28:17Z 2023-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/13655 https://www.sciencedirect.com/science/article/abs/pii/S0378517322009905 0378-5173 1873-3476 https://doi.org/10.1016/j.ijpharm.2022.122435 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I102-001/2019-PD-E5-I102-001/AR./Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública info:eu-repo/semantics/embargoedAccess application/pdf Elsevier International Journal of Pharmaceutics 630 : 122435 (January 2023) |