The Role of Osmolytes and Membrane Lipids in the Adaptation of Acidophilic Fungi
Acidophiles maintain near-neutral intracellular pH using proton pumps. We have suggested the protective role of osmolytes and membrane lipids in the adaptation to an acidic environment. Previously we have observed, for the first time, high levels of trehalose in acidophilic basidiomycete Sistotrema brinkmannii. Here, we have studied the composition of both osmolytes and membrane lipids of two more acidophilic fungi. Trehalose and polyols were among the main osmolytes during growth under optimal conditions (pH 4.0) in basidiomycete Phlebiopsis gigantea and ascomycete Mollisia sp. Phosphatidic acids, phosphatidylethanolamines, phosphatidylcholines, and sterols, were predominant membrane lipids in both fungi. P. gigantea had a narrow optimum of growth at pH 4.0, resulting in a sharp decline of growth rate at pH 2.6 and 5.0, accompanied by a decrease in the number of osmolytes and significant changes in the composition of membrane lipids. In contrast, Mollisia sp. had a broad optimal growth range (pH 3.0–5.0), and the number of osmolytes either stayed the same (at pH 6.0) or increased (at pH 2.6), while membrane lipids composition remained unchanged. Thus, the data obtained indicate the participation of osmolytes and membrane lipids in the adaptation of acidophilic fungi.
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Format: | Article/Letter to editor biblioteca |
Language: | English |
Subjects: | Mollisia, Phlebiopsis gigantea, extremophilic fungi, phosphatidic acids, trehalose, |
Online Access: | https://research.wur.nl/en/publications/the-role-of-osmolytes-and-membrane-lipids-in-the-adaptation-of-ac |
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dig-wur-nl-wurpubs-6184352024-12-04 Ianutsevich, Elena A. Danilova, Olga A. Grum-Grzhimaylo, Olga A. Tereshina, Vera M. Article/Letter to editor Microorganisms 11 (2023) 7 ISSN: 2076-2607 The Role of Osmolytes and Membrane Lipids in the Adaptation of Acidophilic Fungi 2023 Acidophiles maintain near-neutral intracellular pH using proton pumps. We have suggested the protective role of osmolytes and membrane lipids in the adaptation to an acidic environment. Previously we have observed, for the first time, high levels of trehalose in acidophilic basidiomycete Sistotrema brinkmannii. Here, we have studied the composition of both osmolytes and membrane lipids of two more acidophilic fungi. Trehalose and polyols were among the main osmolytes during growth under optimal conditions (pH 4.0) in basidiomycete Phlebiopsis gigantea and ascomycete Mollisia sp. Phosphatidic acids, phosphatidylethanolamines, phosphatidylcholines, and sterols, were predominant membrane lipids in both fungi. P. gigantea had a narrow optimum of growth at pH 4.0, resulting in a sharp decline of growth rate at pH 2.6 and 5.0, accompanied by a decrease in the number of osmolytes and significant changes in the composition of membrane lipids. In contrast, Mollisia sp. had a broad optimal growth range (pH 3.0–5.0), and the number of osmolytes either stayed the same (at pH 6.0) or increased (at pH 2.6), while membrane lipids composition remained unchanged. Thus, the data obtained indicate the participation of osmolytes and membrane lipids in the adaptation of acidophilic fungi. en application/pdf https://research.wur.nl/en/publications/the-role-of-osmolytes-and-membrane-lipids-in-the-adaptation-of-ac 10.3390/microorganisms11071733 https://edepot.wur.nl/637374 Mollisia Phlebiopsis gigantea extremophilic fungi phosphatidic acids trehalose https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/ Wageningen University & Research |
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Mollisia Phlebiopsis gigantea extremophilic fungi phosphatidic acids trehalose Mollisia Phlebiopsis gigantea extremophilic fungi phosphatidic acids trehalose |
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Mollisia Phlebiopsis gigantea extremophilic fungi phosphatidic acids trehalose Mollisia Phlebiopsis gigantea extremophilic fungi phosphatidic acids trehalose Ianutsevich, Elena A. Danilova, Olga A. Grum-Grzhimaylo, Olga A. Tereshina, Vera M. The Role of Osmolytes and Membrane Lipids in the Adaptation of Acidophilic Fungi |
description |
Acidophiles maintain near-neutral intracellular pH using proton pumps. We have suggested the protective role of osmolytes and membrane lipids in the adaptation to an acidic environment. Previously we have observed, for the first time, high levels of trehalose in acidophilic basidiomycete Sistotrema brinkmannii. Here, we have studied the composition of both osmolytes and membrane lipids of two more acidophilic fungi. Trehalose and polyols were among the main osmolytes during growth under optimal conditions (pH 4.0) in basidiomycete Phlebiopsis gigantea and ascomycete Mollisia sp. Phosphatidic acids, phosphatidylethanolamines, phosphatidylcholines, and sterols, were predominant membrane lipids in both fungi. P. gigantea had a narrow optimum of growth at pH 4.0, resulting in a sharp decline of growth rate at pH 2.6 and 5.0, accompanied by a decrease in the number of osmolytes and significant changes in the composition of membrane lipids. In contrast, Mollisia sp. had a broad optimal growth range (pH 3.0–5.0), and the number of osmolytes either stayed the same (at pH 6.0) or increased (at pH 2.6), while membrane lipids composition remained unchanged. Thus, the data obtained indicate the participation of osmolytes and membrane lipids in the adaptation of acidophilic fungi. |
format |
Article/Letter to editor |
topic_facet |
Mollisia Phlebiopsis gigantea extremophilic fungi phosphatidic acids trehalose |
author |
Ianutsevich, Elena A. Danilova, Olga A. Grum-Grzhimaylo, Olga A. Tereshina, Vera M. |
author_facet |
Ianutsevich, Elena A. Danilova, Olga A. Grum-Grzhimaylo, Olga A. Tereshina, Vera M. |
author_sort |
Ianutsevich, Elena A. |
title |
The Role of Osmolytes and Membrane Lipids in the Adaptation of Acidophilic Fungi |
title_short |
The Role of Osmolytes and Membrane Lipids in the Adaptation of Acidophilic Fungi |
title_full |
The Role of Osmolytes and Membrane Lipids in the Adaptation of Acidophilic Fungi |
title_fullStr |
The Role of Osmolytes and Membrane Lipids in the Adaptation of Acidophilic Fungi |
title_full_unstemmed |
The Role of Osmolytes and Membrane Lipids in the Adaptation of Acidophilic Fungi |
title_sort |
role of osmolytes and membrane lipids in the adaptation of acidophilic fungi |
url |
https://research.wur.nl/en/publications/the-role-of-osmolytes-and-membrane-lipids-in-the-adaptation-of-ac |
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