A Phytophthora infestans RXLR effector targets a potato ubiquitin-like domain-containing protein to inhibit the proteasome activity and hamper plant immunity
Ubiquitin-like domain-containing proteins (UDPs) are involved in the ubiquitin-proteasome system because of their ability to interact with the 26S proteasome. Here, we identified potato StUDP as a target of the Phytophthora infestans RXLR effector Pi06432 (PITG_06432), which supresses the salicylic acid (SA)-related immune pathway. By overexpressing and silencing of StUDP in potato, we show that StUDP negatively regulates plant immunity against P. infestans. StUDP interacts with, and destabilizes, the 26S proteasome subunit that is referred to as REGULATORY PARTICLE TRIPLE-A ATP-ASE (RPT) subunit StRPT3b. This destabilization represses the proteasome activity. Proteomic analysis and Western blotting show that StUDP decreases the stability of the master transcription factor SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1) in SA biosynthesis. StUDP negatively regulates the SA signalling pathway by repressing the proteasome activity and destabilizing StSARD1, leading to a decreased expression of the SARD1-targeted gene ISOCHORISMATE SYNTHASE 1 and thereby a decrease in SA content. Pi06432 stabilizes StUDP, and it depends on StUDP to destabilize StRPT3b and thereby supress the proteasome activity. Our study reveals that the P. infestans effector Pi06432 targets StUDP to hamper the homeostasis of the proteasome by the degradation of the proteasome subunit StRPT3b and thereby suppresses SA-related immunity.
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| Format: | Article/Letter to editor biblioteca |
| Language: | English |
| Subjects: | UDP, effector target, late blight disease, oomycete, salicylic acid signalling pathway, |
| Online Access: | https://research.wur.nl/en/publications/a-phytophthora-infestansrxlr-effector-targets-a-potato-ubiquitin- |
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dig-wur-nl-wurpubs-6112482024-12-04 Wang, Ziwei Li, Tingting Zhang, Xiaojiang Feng, Jiashu Liu, Zhuting Shan, Weixing Joosten, Matthieu H.A.J. Govers, Francine Du, Yu Article/Letter to editor New Phytologist 238 (2023) 2 ISSN: 0028-646X A Phytophthora infestans RXLR effector targets a potato ubiquitin-like domain-containing protein to inhibit the proteasome activity and hamper plant immunity 2023 Ubiquitin-like domain-containing proteins (UDPs) are involved in the ubiquitin-proteasome system because of their ability to interact with the 26S proteasome. Here, we identified potato StUDP as a target of the Phytophthora infestans RXLR effector Pi06432 (PITG_06432), which supresses the salicylic acid (SA)-related immune pathway. By overexpressing and silencing of StUDP in potato, we show that StUDP negatively regulates plant immunity against P. infestans. StUDP interacts with, and destabilizes, the 26S proteasome subunit that is referred to as REGULATORY PARTICLE TRIPLE-A ATP-ASE (RPT) subunit StRPT3b. This destabilization represses the proteasome activity. Proteomic analysis and Western blotting show that StUDP decreases the stability of the master transcription factor SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1) in SA biosynthesis. StUDP negatively regulates the SA signalling pathway by repressing the proteasome activity and destabilizing StSARD1, leading to a decreased expression of the SARD1-targeted gene ISOCHORISMATE SYNTHASE 1 and thereby a decrease in SA content. Pi06432 stabilizes StUDP, and it depends on StUDP to destabilize StRPT3b and thereby supress the proteasome activity. Our study reveals that the P. infestans effector Pi06432 targets StUDP to hamper the homeostasis of the proteasome by the degradation of the proteasome subunit StRPT3b and thereby suppresses SA-related immunity. en application/pdf https://research.wur.nl/en/publications/a-phytophthora-infestansrxlr-effector-targets-a-potato-ubiquitin- 10.1111/nph.18749 https://edepot.wur.nl/588208 UDP effector target late blight disease oomycete salicylic acid signalling pathway (c) publisher Wageningen University & Research |
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UDP effector target late blight disease oomycete salicylic acid signalling pathway UDP effector target late blight disease oomycete salicylic acid signalling pathway |
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UDP effector target late blight disease oomycete salicylic acid signalling pathway UDP effector target late blight disease oomycete salicylic acid signalling pathway Wang, Ziwei Li, Tingting Zhang, Xiaojiang Feng, Jiashu Liu, Zhuting Shan, Weixing Joosten, Matthieu H.A.J. Govers, Francine Du, Yu A Phytophthora infestans RXLR effector targets a potato ubiquitin-like domain-containing protein to inhibit the proteasome activity and hamper plant immunity |
| description |
Ubiquitin-like domain-containing proteins (UDPs) are involved in the ubiquitin-proteasome system because of their ability to interact with the 26S proteasome. Here, we identified potato StUDP as a target of the Phytophthora infestans RXLR effector Pi06432 (PITG_06432), which supresses the salicylic acid (SA)-related immune pathway. By overexpressing and silencing of StUDP in potato, we show that StUDP negatively regulates plant immunity against P. infestans. StUDP interacts with, and destabilizes, the 26S proteasome subunit that is referred to as REGULATORY PARTICLE TRIPLE-A ATP-ASE (RPT) subunit StRPT3b. This destabilization represses the proteasome activity. Proteomic analysis and Western blotting show that StUDP decreases the stability of the master transcription factor SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1) in SA biosynthesis. StUDP negatively regulates the SA signalling pathway by repressing the proteasome activity and destabilizing StSARD1, leading to a decreased expression of the SARD1-targeted gene ISOCHORISMATE SYNTHASE 1 and thereby a decrease in SA content. Pi06432 stabilizes StUDP, and it depends on StUDP to destabilize StRPT3b and thereby supress the proteasome activity. Our study reveals that the P. infestans effector Pi06432 targets StUDP to hamper the homeostasis of the proteasome by the degradation of the proteasome subunit StRPT3b and thereby suppresses SA-related immunity. |
| format |
Article/Letter to editor |
| topic_facet |
UDP effector target late blight disease oomycete salicylic acid signalling pathway |
| author |
Wang, Ziwei Li, Tingting Zhang, Xiaojiang Feng, Jiashu Liu, Zhuting Shan, Weixing Joosten, Matthieu H.A.J. Govers, Francine Du, Yu |
| author_facet |
Wang, Ziwei Li, Tingting Zhang, Xiaojiang Feng, Jiashu Liu, Zhuting Shan, Weixing Joosten, Matthieu H.A.J. Govers, Francine Du, Yu |
| author_sort |
Wang, Ziwei |
| title |
A Phytophthora infestans RXLR effector targets a potato ubiquitin-like domain-containing protein to inhibit the proteasome activity and hamper plant immunity |
| title_short |
A Phytophthora infestans RXLR effector targets a potato ubiquitin-like domain-containing protein to inhibit the proteasome activity and hamper plant immunity |
| title_full |
A Phytophthora infestans RXLR effector targets a potato ubiquitin-like domain-containing protein to inhibit the proteasome activity and hamper plant immunity |
| title_fullStr |
A Phytophthora infestans RXLR effector targets a potato ubiquitin-like domain-containing protein to inhibit the proteasome activity and hamper plant immunity |
| title_full_unstemmed |
A Phytophthora infestans RXLR effector targets a potato ubiquitin-like domain-containing protein to inhibit the proteasome activity and hamper plant immunity |
| title_sort |
phytophthora infestans rxlr effector targets a potato ubiquitin-like domain-containing protein to inhibit the proteasome activity and hamper plant immunity |
| url |
https://research.wur.nl/en/publications/a-phytophthora-infestansrxlr-effector-targets-a-potato-ubiquitin- |
| work_keys_str_mv |
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