Optical microscope for spectrally resolved imaging and method for such a microscope

A microscope includes a light source, a sample holder, an optical imaging system and a digital image sensor. The sample holder is arranged for holding a sample containing spectral emitters with specific emission profiles. The light source is configured for emitting a light beam for excitation of emitters in the sample. The optical imaging system is arranged for collecting radiation from emitters in the sample and imaging an emitters' image from the collected radiation through the optical imaging system on the digital image sensor. The microscope includes a diffractive optical element mounted directly in front of the digital image sensor at a first distance. The diffractive optical element is configured for receiving the emitters' image and creating a diffraction pattern thereof of at least two beams of different order. The first distance is chosen such that the at least two beams are directly projected on the digital image sensor.

Bibliographic Details

Main Authors:	Martens, Koen Jacobus Adrianus, Hohlbein, Johannes
Format:	Patent biblioteca
Language:	English
Subjects:	Life Science,
Online Access:	https://research.wur.nl/en/publications/optical-microscope-for-spectrally-resolved-imaging-and-method-for
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