The Induction of an Effective dsRNA-Mediated Resistance Against Tomato Spotted Wilt Virus by Exogenous Application of Double-Stranded RNA Largely Depends on the Selection of the Viral RNA Target Region
Tomato spotted wilt virus (TSWV) is a devastating plant pathogen, causing huge crop losses worldwide. Unfortunately, due to its wide host range and emergence of resistance breaking strains, its management is challenging. Up to now, resistance to TSWV infection based on RNA interference (RNAi) has been achieved only in transgenic plants expressing parts of the viral genome or artificial microRNAs targeting it. Exogenous application of double-stranded RNAs (dsRNAs) for inducing virus resistance in plants, namely RNAi-based vaccination, represents an attractive and promising alternative, already shown to be effective against different positive-sense RNA viruses and viroids. In the present study, the protection efficacy of exogenous application of dsRNAs targeting the nucleocapsid (N) or the movement protein (NSm) coding genes of the negative-sense RNA virus TSWV was evaluated in Nicotiana benthamiana as model plant and in tomato as economically important crop. Most of the plants treated with N-targeting dsRNAs, but not with NSm-targeting dsRNAs, remained asymptomatic until 40 (N. benthamiana) and 63 (tomato) dpi, while the remaining ones showed a significant delay in systemic symptoms appearance. The different efficacy of N- and NSm-targeting dsRNAs in protecting plants is discussed in the light of their processing, mobility and biological role. These results indicate that the RNAi-based vaccination is effective also against negative-sense RNA viruses but emphasize that the choice of the target viral sequence in designing RNAi-based vaccines is crucial for its success.
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| Format: | Article/Letter to editor biblioteca |
| Language: | English |
| Subjects: | RNAi-based vaccination, ambisense RNA, cell-to-cell movement protein, double-stranded rnas, nucleocapsid protein, orthotospovirus, |
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dig-wur-nl-wurpubs-5754482024-10-02 Tabein, Saeid Jansen, Marco Noris, Emanuela Vaira, Anna Maria Marian, Daniele Behjatnia, Ali Akbar Accotto, Gian Paolo Miozzi, Laura Article/Letter to editor Frontiers in Plant Science 11 (2020) ISSN: 1664-462X The Induction of an Effective dsRNA-Mediated Resistance Against Tomato Spotted Wilt Virus by Exogenous Application of Double-Stranded RNA Largely Depends on the Selection of the Viral RNA Target Region 2020 Tomato spotted wilt virus (TSWV) is a devastating plant pathogen, causing huge crop losses worldwide. Unfortunately, due to its wide host range and emergence of resistance breaking strains, its management is challenging. Up to now, resistance to TSWV infection based on RNA interference (RNAi) has been achieved only in transgenic plants expressing parts of the viral genome or artificial microRNAs targeting it. Exogenous application of double-stranded RNAs (dsRNAs) for inducing virus resistance in plants, namely RNAi-based vaccination, represents an attractive and promising alternative, already shown to be effective against different positive-sense RNA viruses and viroids. In the present study, the protection efficacy of exogenous application of dsRNAs targeting the nucleocapsid (N) or the movement protein (NSm) coding genes of the negative-sense RNA virus TSWV was evaluated in Nicotiana benthamiana as model plant and in tomato as economically important crop. Most of the plants treated with N-targeting dsRNAs, but not with NSm-targeting dsRNAs, remained asymptomatic until 40 (N. benthamiana) and 63 (tomato) dpi, while the remaining ones showed a significant delay in systemic symptoms appearance. The different efficacy of N- and NSm-targeting dsRNAs in protecting plants is discussed in the light of their processing, mobility and biological role. These results indicate that the RNAi-based vaccination is effective also against negative-sense RNA viruses but emphasize that the choice of the target viral sequence in designing RNAi-based vaccines is crucial for its success. en application/pdf https://research.wur.nl/en/publications/the-induction-of-an-effective-dsrna-mediated-resistance-against-t 10.3389/fpls.2020.533338 https://edepot.wur.nl/538060 RNAi-based vaccination ambisense RNA cell-to-cell movement protein double-stranded rnas nucleocapsid protein orthotospovirus https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/ Wageningen University & Research |
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RNAi-based vaccination ambisense RNA cell-to-cell movement protein double-stranded rnas nucleocapsid protein orthotospovirus RNAi-based vaccination ambisense RNA cell-to-cell movement protein double-stranded rnas nucleocapsid protein orthotospovirus |
| spellingShingle |
RNAi-based vaccination ambisense RNA cell-to-cell movement protein double-stranded rnas nucleocapsid protein orthotospovirus RNAi-based vaccination ambisense RNA cell-to-cell movement protein double-stranded rnas nucleocapsid protein orthotospovirus Tabein, Saeid Jansen, Marco Noris, Emanuela Vaira, Anna Maria Marian, Daniele Behjatnia, Ali Akbar Accotto, Gian Paolo Miozzi, Laura The Induction of an Effective dsRNA-Mediated Resistance Against Tomato Spotted Wilt Virus by Exogenous Application of Double-Stranded RNA Largely Depends on the Selection of the Viral RNA Target Region |
| description |
Tomato spotted wilt virus (TSWV) is a devastating plant pathogen, causing huge crop losses worldwide. Unfortunately, due to its wide host range and emergence of resistance breaking strains, its management is challenging. Up to now, resistance to TSWV infection based on RNA interference (RNAi) has been achieved only in transgenic plants expressing parts of the viral genome or artificial microRNAs targeting it. Exogenous application of double-stranded RNAs (dsRNAs) for inducing virus resistance in plants, namely RNAi-based vaccination, represents an attractive and promising alternative, already shown to be effective against different positive-sense RNA viruses and viroids. In the present study, the protection efficacy of exogenous application of dsRNAs targeting the nucleocapsid (N) or the movement protein (NSm) coding genes of the negative-sense RNA virus TSWV was evaluated in Nicotiana benthamiana as model plant and in tomato as economically important crop. Most of the plants treated with N-targeting dsRNAs, but not with NSm-targeting dsRNAs, remained asymptomatic until 40 (N. benthamiana) and 63 (tomato) dpi, while the remaining ones showed a significant delay in systemic symptoms appearance. The different efficacy of N- and NSm-targeting dsRNAs in protecting plants is discussed in the light of their processing, mobility and biological role. These results indicate that the RNAi-based vaccination is effective also against negative-sense RNA viruses but emphasize that the choice of the target viral sequence in designing RNAi-based vaccines is crucial for its success. |
| format |
Article/Letter to editor |
| topic_facet |
RNAi-based vaccination ambisense RNA cell-to-cell movement protein double-stranded rnas nucleocapsid protein orthotospovirus |
| author |
Tabein, Saeid Jansen, Marco Noris, Emanuela Vaira, Anna Maria Marian, Daniele Behjatnia, Ali Akbar Accotto, Gian Paolo Miozzi, Laura |
| author_facet |
Tabein, Saeid Jansen, Marco Noris, Emanuela Vaira, Anna Maria Marian, Daniele Behjatnia, Ali Akbar Accotto, Gian Paolo Miozzi, Laura |
| author_sort |
Tabein, Saeid |
| title |
The Induction of an Effective dsRNA-Mediated Resistance Against Tomato Spotted Wilt Virus by Exogenous Application of Double-Stranded RNA Largely Depends on the Selection of the Viral RNA Target Region |
| title_short |
The Induction of an Effective dsRNA-Mediated Resistance Against Tomato Spotted Wilt Virus by Exogenous Application of Double-Stranded RNA Largely Depends on the Selection of the Viral RNA Target Region |
| title_full |
The Induction of an Effective dsRNA-Mediated Resistance Against Tomato Spotted Wilt Virus by Exogenous Application of Double-Stranded RNA Largely Depends on the Selection of the Viral RNA Target Region |
| title_fullStr |
The Induction of an Effective dsRNA-Mediated Resistance Against Tomato Spotted Wilt Virus by Exogenous Application of Double-Stranded RNA Largely Depends on the Selection of the Viral RNA Target Region |
| title_full_unstemmed |
The Induction of an Effective dsRNA-Mediated Resistance Against Tomato Spotted Wilt Virus by Exogenous Application of Double-Stranded RNA Largely Depends on the Selection of the Viral RNA Target Region |
| title_sort |
induction of an effective dsrna-mediated resistance against tomato spotted wilt virus by exogenous application of double-stranded rna largely depends on the selection of the viral rna target region |
| url |
https://research.wur.nl/en/publications/the-induction-of-an-effective-dsrna-mediated-resistance-against-t |
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