Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein

Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein

Virulence of Newcastle disease virus (NDV) is mainly determined by the amino acid sequence surrounding the fusion (F) protein cleavage site, since host proteases that cleave the F protein of virulent strains are present in more tissues than those that cleave the F protein of non-virulent strains. Nevertheless, comparison of NDV strains that carry exactly the same F protein cleavage site shows that significant differences in virulence still exist. For instance, virulent field strain Herts/33 with the F cleavage site 112RRQRRF117 had an intracerebral pathogenicity index of 1.88 compared with 1.28 for strain NDFLtag, which has the same cleavage site. This implies that additional factors contribute to virulence. After generating an infectious clone of Herts/33 (FL-Herts), we were able to map the location of additional virulence factors by exchanging sequences between FL-Herts and NDFLtag. The results showed that, in addition to the F protein cleavage site, the haemagglutinin-neuraminidase (HN) protein also contributed to virulence. The effect of the HN protein on virulence was most prominent after intravenous inoculation. Interestingly, both the stem region and the globular head of the HN protein seem to be involved in determining virulence

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Main Authors: de Leeuw, O.S., Koch, G., Hartog, L., Ravenshorst, N., Peeters, B.P.H.
Format: Article/Letter to editor biblioteca
Language:English
Subjects:expression, f-protein, generation, glycoproteins, interferon-antagonist, intergenic regions, nucleotide-sequence, pathogenesis, pathogenicity, virus v-protein,
Online Access:https://research.wur.nl/en/publications/virulence-of-newcastle-disease-virus-is-determined-by-the-cleavag
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spelling dig-wur-nl-wurpubs-3424572025-01-23 de Leeuw, O.S. Koch, G. Hartog, L. Ravenshorst, N. Peeters, B.P.H. Article/Letter to editor Journal of General Virology 86 (2005) 6 ISSN: 0022-1317 Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein 2005 Virulence of Newcastle disease virus (NDV) is mainly determined by the amino acid sequence surrounding the fusion (F) protein cleavage site, since host proteases that cleave the F protein of virulent strains are present in more tissues than those that cleave the F protein of non-virulent strains. Nevertheless, comparison of NDV strains that carry exactly the same F protein cleavage site shows that significant differences in virulence still exist. For instance, virulent field strain Herts/33 with the F cleavage site 112RRQRRF117 had an intracerebral pathogenicity index of 1.88 compared with 1.28 for strain NDFLtag, which has the same cleavage site. This implies that additional factors contribute to virulence. After generating an infectious clone of Herts/33 (FL-Herts), we were able to map the location of additional virulence factors by exchanging sequences between FL-Herts and NDFLtag. The results showed that, in addition to the F protein cleavage site, the haemagglutinin-neuraminidase (HN) protein also contributed to virulence. The effect of the HN protein on virulence was most prominent after intravenous inoculation. Interestingly, both the stem region and the globular head of the HN protein seem to be involved in determining virulence en application/pdf https://research.wur.nl/en/publications/virulence-of-newcastle-disease-virus-is-determined-by-the-cleavag 10.1099/vir.0.80822-0 https://edepot.wur.nl/32343 expression f-protein generation glycoproteins interferon-antagonist intergenic regions nucleotide-sequence pathogenesis pathogenicity virus v-protein Wageningen University & Research
institution WUR NL
collection DSpace
country Países bajos
countrycode NL
component Bibliográfico
access En linea
databasecode dig-wur-nl
tag biblioteca
region Europa del Oeste
libraryname WUR Library Netherlands
language English
topic expression
f-protein
generation
glycoproteins
interferon-antagonist
intergenic regions
nucleotide-sequence
pathogenesis
pathogenicity
virus v-protein
expression
f-protein
generation
glycoproteins
interferon-antagonist
intergenic regions
nucleotide-sequence
pathogenesis
pathogenicity
virus v-protein
spellingShingle expression
f-protein
generation
glycoproteins
interferon-antagonist
intergenic regions
nucleotide-sequence
pathogenesis
pathogenicity
virus v-protein
expression
f-protein
generation
glycoproteins
interferon-antagonist
intergenic regions
nucleotide-sequence
pathogenesis
pathogenicity
virus v-protein
de Leeuw, O.S.
Koch, G.
Hartog, L.
Ravenshorst, N.
Peeters, B.P.H.
Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein
description Virulence of Newcastle disease virus (NDV) is mainly determined by the amino acid sequence surrounding the fusion (F) protein cleavage site, since host proteases that cleave the F protein of virulent strains are present in more tissues than those that cleave the F protein of non-virulent strains. Nevertheless, comparison of NDV strains that carry exactly the same F protein cleavage site shows that significant differences in virulence still exist. For instance, virulent field strain Herts/33 with the F cleavage site 112RRQRRF117 had an intracerebral pathogenicity index of 1.88 compared with 1.28 for strain NDFLtag, which has the same cleavage site. This implies that additional factors contribute to virulence. After generating an infectious clone of Herts/33 (FL-Herts), we were able to map the location of additional virulence factors by exchanging sequences between FL-Herts and NDFLtag. The results showed that, in addition to the F protein cleavage site, the haemagglutinin-neuraminidase (HN) protein also contributed to virulence. The effect of the HN protein on virulence was most prominent after intravenous inoculation. Interestingly, both the stem region and the globular head of the HN protein seem to be involved in determining virulence
format Article/Letter to editor
topic_facet expression
f-protein
generation
glycoproteins
interferon-antagonist
intergenic regions
nucleotide-sequence
pathogenesis
pathogenicity
virus v-protein
author de Leeuw, O.S.
Koch, G.
Hartog, L.
Ravenshorst, N.
Peeters, B.P.H.
author_facet de Leeuw, O.S.
Koch, G.
Hartog, L.
Ravenshorst, N.
Peeters, B.P.H.
author_sort de Leeuw, O.S.
title Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein
title_short Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein
title_full Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein
title_fullStr Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein
title_full_unstemmed Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein
title_sort virulence of newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein
url https://research.wur.nl/en/publications/virulence-of-newcastle-disease-virus-is-determined-by-the-cleavag
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AT hartogl virulenceofnewcastlediseasevirusisdeterminedbythecleavagesiteofthefusionproteinandbyboththestemregionandglobularheadofthehaemagglutininneuraminidaseprotein
AT ravenshorstn virulenceofnewcastlediseasevirusisdeterminedbythecleavagesiteofthefusionproteinandbyboththestemregionandglobularheadofthehaemagglutininneuraminidaseprotein
AT peetersbph virulenceofnewcastlediseasevirusisdeterminedbythecleavagesiteofthefusionproteinandbyboththestemregionandglobularheadofthehaemagglutininneuraminidaseprotein
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