Single pass cDNA sequencing - a powerful tool to analyse gene expression in preparasytic juveniles of the southern root-knot nematode Meliodogyne incognita
Expressed sequence tags (EST) have been widely used to assist in gene discovery in various organisms (e.g., Arabidopsis thaliana, Caenorhabditis elegans, Mus musculus, and Homo sapiens). In this paper we describe an EST project, which aims to investigate gene expression in Meloidogyne incognita at the onset of parasitism. Approximately 1000 5′-end sequence tags were produced from a cDNA library made of freshly hatched preparasitic second stage juveniles (J2). The EST were identified in the primary transformants of the cDNA library, and assigned to nine different functional groups, including (candidate) parasitism genes. A large fraction of the EST (45€did not have a putative homologue in public databases. Sixty five percent of the EST that could be clustered into a functional group had putative homologues in other nematode species. EST were found for virtually all parasitism related genes that have been cloned from M. incognita to date. In addition, several novel genes were tagged, including a xylanase and a chitinase gene. The efficiency of EST projects, which produce sequence data for thousands of genes in months time without any difficult pre-selections of mRNA pools, makes random sequencing cDNA libraries a superior method to identify candidates for parasitism related genes in plant-parasitic nematodes. The sequences in this paper are retrievable from Genbank with the accession numbers BE191640 to BE191741, BE217592 to BE217720, BE225324 to BE225598, BE238852 to BE239221, and BE240829 to BE240865
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| Language: | English |
| Subjects: | Chitinase, Parasitism gene, Plant parasitic nematode, Random sequencing, Secretion, |
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dig-wur-nl-wurpubs-3220502024-09-23 Dautova, M. Rosso, M.N. Abad, P. Gommers, F.L. Bakker, J. Smant, G. Article/Letter to editor Nematology 3 (2001) 2 ISSN: 1388-5545 Single pass cDNA sequencing - a powerful tool to analyse gene expression in preparasytic juveniles of the southern root-knot nematode Meliodogyne incognita 2001 Expressed sequence tags (EST) have been widely used to assist in gene discovery in various organisms (e.g., Arabidopsis thaliana, Caenorhabditis elegans, Mus musculus, and Homo sapiens). In this paper we describe an EST project, which aims to investigate gene expression in Meloidogyne incognita at the onset of parasitism. Approximately 1000 5′-end sequence tags were produced from a cDNA library made of freshly hatched preparasitic second stage juveniles (J2). The EST were identified in the primary transformants of the cDNA library, and assigned to nine different functional groups, including (candidate) parasitism genes. A large fraction of the EST (45€did not have a putative homologue in public databases. Sixty five percent of the EST that could be clustered into a functional group had putative homologues in other nematode species. EST were found for virtually all parasitism related genes that have been cloned from M. incognita to date. In addition, several novel genes were tagged, including a xylanase and a chitinase gene. The efficiency of EST projects, which produce sequence data for thousands of genes in months time without any difficult pre-selections of mRNA pools, makes random sequencing cDNA libraries a superior method to identify candidates for parasitism related genes in plant-parasitic nematodes. The sequences in this paper are retrievable from Genbank with the accession numbers BE191640 to BE191741, BE217592 to BE217720, BE225324 to BE225598, BE238852 to BE239221, and BE240829 to BE240865 en application/pdf https://research.wur.nl/en/publications/single-pass-cdna-sequencing-a-powerful-tool-to-analyse-gene-expre 10.1163/156854101750236259 https://edepot.wur.nl/44510 Chitinase Parasitism gene Plant parasitic nematode Random sequencing Secretion Wageningen University & Research |
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Chitinase Parasitism gene Plant parasitic nematode Random sequencing Secretion Chitinase Parasitism gene Plant parasitic nematode Random sequencing Secretion |
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Chitinase Parasitism gene Plant parasitic nematode Random sequencing Secretion Chitinase Parasitism gene Plant parasitic nematode Random sequencing Secretion Dautova, M. Rosso, M.N. Abad, P. Gommers, F.L. Bakker, J. Smant, G. Single pass cDNA sequencing - a powerful tool to analyse gene expression in preparasytic juveniles of the southern root-knot nematode Meliodogyne incognita |
| description |
Expressed sequence tags (EST) have been widely used to assist in gene discovery in various organisms (e.g., Arabidopsis thaliana, Caenorhabditis elegans, Mus musculus, and Homo sapiens). In this paper we describe an EST project, which aims to investigate gene expression in Meloidogyne incognita at the onset of parasitism. Approximately 1000 5′-end sequence tags were produced from a cDNA library made of freshly hatched preparasitic second stage juveniles (J2). The EST were identified in the primary transformants of the cDNA library, and assigned to nine different functional groups, including (candidate) parasitism genes. A large fraction of the EST (45€did not have a putative homologue in public databases. Sixty five percent of the EST that could be clustered into a functional group had putative homologues in other nematode species. EST were found for virtually all parasitism related genes that have been cloned from M. incognita to date. In addition, several novel genes were tagged, including a xylanase and a chitinase gene. The efficiency of EST projects, which produce sequence data for thousands of genes in months time without any difficult pre-selections of mRNA pools, makes random sequencing cDNA libraries a superior method to identify candidates for parasitism related genes in plant-parasitic nematodes. The sequences in this paper are retrievable from Genbank with the accession numbers BE191640 to BE191741, BE217592 to BE217720, BE225324 to BE225598, BE238852 to BE239221, and BE240829 to BE240865 |
| format |
Article/Letter to editor |
| topic_facet |
Chitinase Parasitism gene Plant parasitic nematode Random sequencing Secretion |
| author |
Dautova, M. Rosso, M.N. Abad, P. Gommers, F.L. Bakker, J. Smant, G. |
| author_facet |
Dautova, M. Rosso, M.N. Abad, P. Gommers, F.L. Bakker, J. Smant, G. |
| author_sort |
Dautova, M. |
| title |
Single pass cDNA sequencing - a powerful tool to analyse gene expression in preparasytic juveniles of the southern root-knot nematode Meliodogyne incognita |
| title_short |
Single pass cDNA sequencing - a powerful tool to analyse gene expression in preparasytic juveniles of the southern root-knot nematode Meliodogyne incognita |
| title_full |
Single pass cDNA sequencing - a powerful tool to analyse gene expression in preparasytic juveniles of the southern root-knot nematode Meliodogyne incognita |
| title_fullStr |
Single pass cDNA sequencing - a powerful tool to analyse gene expression in preparasytic juveniles of the southern root-knot nematode Meliodogyne incognita |
| title_full_unstemmed |
Single pass cDNA sequencing - a powerful tool to analyse gene expression in preparasytic juveniles of the southern root-knot nematode Meliodogyne incognita |
| title_sort |
single pass cdna sequencing - a powerful tool to analyse gene expression in preparasytic juveniles of the southern root-knot nematode meliodogyne incognita |
| url |
https://research.wur.nl/en/publications/single-pass-cdna-sequencing-a-powerful-tool-to-analyse-gene-expre |
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