Pathological, immunological and parasitological study of sheep vaccinated with the recombinant protein 14-3-3z and experimentally infected with Fasciola hepatica
14 páginas, 4 figuras, 2 tablas
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
2018
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| Subjects: | Fasciola hepatica, Immunisation, Montanide, Vaccine, rFh14-3-3z, vaccines, immunization, |
| Online Access: | http://hdl.handle.net/10261/345243 http://dx.doi.org/10.13039/501100003329 https://api.elsevier.com/content/abstract/scopus_id/85049602939 |
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dig-irnasa-es-10261-3452432024-05-18T20:38:17Z Pathological, immunological and parasitological study of sheep vaccinated with the recombinant protein 14-3-3z and experimentally infected with Fasciola hepatica Pérez-Caballero, R. Siles Lucas, Mar González Miguel, Javier Martínez-Moreno, F. J. Escamilla, A. Pérez, J. Martínez-Moreno, A. Buffoni, L. Ministerio de Economía y Competitividad (España) Siles Lucas, Mar [0000-0002-1257-2562] González Miguel, Javier [0000-0003-4279-4761] Fasciola hepatica Immunisation Montanide Vaccine rFh14-3-3z Fasciola hepatica vaccines immunization 14 páginas, 4 figuras, 2 tablas In this study, the immunogenicity and protective capacity of a new recombinant vaccine candidate, the rFh14-3-3z protein was analysed in sheep experimentally challenged with Fasciola hepatica, in terms of fluke burden, faecal egg counts, hepatic damage and humoral immune response. Three groups of 8 animals each were used for study, group 1 was immunised with the rFh14-3-3z in Montanide adjuvant, whereas group 2 and 3 remained as adjuvant control and infection control groups, respectively. The parasitological analysis showed that no significant reduction in fluke burden, fluke size and faecal egg counts was detected. The extent of hepatic damage was very similar between groups. Nonetheless, animals immunised with the rFh14-3-3z protein induced the development of specific IgG1 and IgG2, being the IgG1 the predominant antibody; which confirms the immunogenicity of this protein in sheep. This is the first report of the 14-3-3z proteins as vaccine against the infection with F. hepatica. This work was supported by grants AGL2002-00644, AGL2015-67023-C2-1-R/2-R from the Spanish Ministry of Economy, Industry and Competitiveness Peer reviewed 2024-02-05T07:28:21Z 2024-02-05T07:28:21Z 2018 artículo artículo Veterinary Immunology and Immunopathology 202: 115-121 (2018) 0165-2427 http://hdl.handle.net/10261/345243 10.1016/j.vetimm.2018.07.006 http://dx.doi.org/10.13039/501100003329 30078585 2-s2.0-85049602939 https://api.elsevier.com/content/abstract/scopus_id/85049602939 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO// AGL2015-67023-C2-1-R/2-R Veterinary immunology and immunopathology Postprint http://dx.doi.org/10.1016/j.vetimm.2018.07.006 Sí open Elsevier |
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biblioteca |
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Fasciola hepatica Immunisation Montanide Vaccine rFh14-3-3z Fasciola hepatica vaccines immunization Fasciola hepatica Immunisation Montanide Vaccine rFh14-3-3z Fasciola hepatica vaccines immunization |
| spellingShingle |
Fasciola hepatica Immunisation Montanide Vaccine rFh14-3-3z Fasciola hepatica vaccines immunization Fasciola hepatica Immunisation Montanide Vaccine rFh14-3-3z Fasciola hepatica vaccines immunization Pérez-Caballero, R. Siles Lucas, Mar González Miguel, Javier Martínez-Moreno, F. J. Escamilla, A. Pérez, J. Martínez-Moreno, A. Buffoni, L. Pathological, immunological and parasitological study of sheep vaccinated with the recombinant protein 14-3-3z and experimentally infected with Fasciola hepatica |
| description |
14 páginas, 4 figuras, 2 tablas |
| author2 |
Ministerio de Economía y Competitividad (España) |
| author_facet |
Ministerio de Economía y Competitividad (España) Pérez-Caballero, R. Siles Lucas, Mar González Miguel, Javier Martínez-Moreno, F. J. Escamilla, A. Pérez, J. Martínez-Moreno, A. Buffoni, L. |
| format |
artículo |
| topic_facet |
Fasciola hepatica Immunisation Montanide Vaccine rFh14-3-3z Fasciola hepatica vaccines immunization |
| author |
Pérez-Caballero, R. Siles Lucas, Mar González Miguel, Javier Martínez-Moreno, F. J. Escamilla, A. Pérez, J. Martínez-Moreno, A. Buffoni, L. |
| author_sort |
Pérez-Caballero, R. |
| title |
Pathological, immunological and parasitological study of sheep vaccinated with the recombinant protein 14-3-3z and experimentally infected with Fasciola hepatica |
| title_short |
Pathological, immunological and parasitological study of sheep vaccinated with the recombinant protein 14-3-3z and experimentally infected with Fasciola hepatica |
| title_full |
Pathological, immunological and parasitological study of sheep vaccinated with the recombinant protein 14-3-3z and experimentally infected with Fasciola hepatica |
| title_fullStr |
Pathological, immunological and parasitological study of sheep vaccinated with the recombinant protein 14-3-3z and experimentally infected with Fasciola hepatica |
| title_full_unstemmed |
Pathological, immunological and parasitological study of sheep vaccinated with the recombinant protein 14-3-3z and experimentally infected with Fasciola hepatica |
| title_sort |
pathological, immunological and parasitological study of sheep vaccinated with the recombinant protein 14-3-3z and experimentally infected with fasciola hepatica |
| publisher |
Elsevier |
| publishDate |
2018 |
| url |
http://hdl.handle.net/10261/345243 http://dx.doi.org/10.13039/501100003329 https://api.elsevier.com/content/abstract/scopus_id/85049602939 |
| work_keys_str_mv |
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1802820160103383040 |