Evaluation of the recombinant antigens B2t and 2B2t, compared with hydatid fluid, in IgGELISA and immunostrips for the diagnosis and follow up of CE patients

Evaluation of the recombinant antigens B2t and 2B2t, compared with hydatid fluid, in IgGELISA and immunostrips for the diagnosis and follow up of CE patients

21 páginas, 5 figuras y 7 tablas.

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Main Authors: Hernández González, Ana, Sánchez Ovejero, Carlos, Manzano Román, Raúl, González Sánchez, María, Delgado, José Manuel, Pardo García, Teresa, Soriano-Gálvez, Francisco, Akhan, Okan, Cretu, Carmen Michaela, Vutova, Kamenna, Tamarozzi, Francesca, Mariconti, Mara, Brunetti, Enrico, Vola, Ambra, Fabiani, Massimo, Casulli, Adriano, Siles Lucas, Mar
Other Authors: European Commission
Format: artículo biblioteca
Language:English
Published: Public Library of Science 2018
Subjects:Antigens, Cystic echinococcosis, Immunostrips, ELISA, Echinococcus granulosus,
Online Access:http://hdl.handle.net/10261/178623
https://doi.org/10.1371/journal.pntd.0006741
http://dx.doi.org/10.13039/501100000780
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id dig-irnasa-es-10261-178623
record_format koha
institution IRNASA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-irnasa-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IRNASA España
language English
topic Antigens
Cystic echinococcosis
Immunostrips
ELISA
Echinococcus granulosus
Antigens
Cystic echinococcosis
Immunostrips
ELISA
Echinococcus granulosus
spellingShingle Antigens
Cystic echinococcosis
Immunostrips
ELISA
Echinococcus granulosus
Antigens
Cystic echinococcosis
Immunostrips
ELISA
Echinococcus granulosus
Hernández González, Ana
Sánchez Ovejero, Carlos
Manzano Román, Raúl
González Sánchez, María
Delgado, José Manuel
Pardo García, Teresa
Soriano-Gálvez, Francisco
Akhan, Okan
Cretu, Carmen Michaela
Vutova, Kamenna
Tamarozzi, Francesca
Mariconti, Mara
Brunetti, Enrico
Vola, Ambra
Fabiani, Massimo
Casulli, Adriano
Siles Lucas, Mar
Evaluation of the recombinant antigens B2t and 2B2t, compared with hydatid fluid, in IgGELISA and immunostrips for the diagnosis and follow up of CE patients
description 21 páginas, 5 figuras y 7 tablas.
author2 European Commission
author_facet European Commission
Hernández González, Ana
Sánchez Ovejero, Carlos
Manzano Román, Raúl
González Sánchez, María
Delgado, José Manuel
Pardo García, Teresa
Soriano-Gálvez, Francisco
Akhan, Okan
Cretu, Carmen Michaela
Vutova, Kamenna
Tamarozzi, Francesca
Mariconti, Mara
Brunetti, Enrico
Vola, Ambra
Fabiani, Massimo
Casulli, Adriano
Siles Lucas, Mar
format artículo
topic_facet Antigens
Cystic echinococcosis
Immunostrips
ELISA
Echinococcus granulosus
author Hernández González, Ana
Sánchez Ovejero, Carlos
Manzano Román, Raúl
González Sánchez, María
Delgado, José Manuel
Pardo García, Teresa
Soriano-Gálvez, Francisco
Akhan, Okan
Cretu, Carmen Michaela
Vutova, Kamenna
Tamarozzi, Francesca
Mariconti, Mara
Brunetti, Enrico
Vola, Ambra
Fabiani, Massimo
Casulli, Adriano
Siles Lucas, Mar
author_sort Hernández González, Ana
title Evaluation of the recombinant antigens B2t and 2B2t, compared with hydatid fluid, in IgGELISA and immunostrips for the diagnosis and follow up of CE patients
title_short Evaluation of the recombinant antigens B2t and 2B2t, compared with hydatid fluid, in IgGELISA and immunostrips for the diagnosis and follow up of CE patients
title_full Evaluation of the recombinant antigens B2t and 2B2t, compared with hydatid fluid, in IgGELISA and immunostrips for the diagnosis and follow up of CE patients
title_fullStr Evaluation of the recombinant antigens B2t and 2B2t, compared with hydatid fluid, in IgGELISA and immunostrips for the diagnosis and follow up of CE patients
title_full_unstemmed Evaluation of the recombinant antigens B2t and 2B2t, compared with hydatid fluid, in IgGELISA and immunostrips for the diagnosis and follow up of CE patients
title_sort evaluation of the recombinant antigens b2t and 2b2t, compared with hydatid fluid, in iggelisa and immunostrips for the diagnosis and follow up of ce patients
publisher Public Library of Science
publishDate 2018
url http://hdl.handle.net/10261/178623
https://doi.org/10.1371/journal.pntd.0006741
http://dx.doi.org/10.13039/501100000780
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spelling dig-irnasa-es-10261-1786232022-03-21T15:30:45Z Evaluation of the recombinant antigens B2t and 2B2t, compared with hydatid fluid, in IgGELISA and immunostrips for the diagnosis and follow up of CE patients Hernández González, Ana Sánchez Ovejero, Carlos Manzano Román, Raúl González Sánchez, María Delgado, José Manuel Pardo García, Teresa Soriano-Gálvez, Francisco Akhan, Okan Cretu, Carmen Michaela Vutova, Kamenna Tamarozzi, Francesca Mariconti, Mara Brunetti, Enrico Vola, Ambra Fabiani, Massimo Casulli, Adriano Siles Lucas, Mar European Commission Hernández González, Ana [0000-0002-8019-7354] Siles-Lucas, Mar [0000-0002-1257-2562] Antigens Cystic echinococcosis Immunostrips ELISA Echinococcus granulosus 21 páginas, 5 figuras y 7 tablas. Cystic echinococcosis (CE) is one of the most widespread helminthic zoonoses and is caused by the tapeworm Echinococcus granulosus complex. CE diagnosis and monitoring primarily rely on imaging techniques, complemented by serology. This is usually approached by the detection of IgG antibodies against hydatid fluid (HF), but the use of this heterogeneous antigenic mixture results in a variable percentage of false positive and negative results, and has shown to be useless for follow-up due to the long persistence of anti-HF antibodies in cured patients. To improve test performances and standardization, a number of recombinant antigens mainly derived from HF have been described, among them the B2t and 2B2t antigens. The performance of these antigens in the diagnosis and follow up of patients with CE has been so far evaluated on a limited number of samples. Here, we evaluated the performances of tests based on B2t and 2B2t recombinant antigens compared to HF in IgG-ELISA and immunochromatography (IC) for the diagnosis and follow-up of patients with CE in a retrospective cohort study. A total of 721 serum samples were collected: 587 from 253 patients with CE diagnosed by ultrasonography (US), 42 from patients with alveolar echinococcosis and 92 from healthy donors from Salamanca (Spain). The highest overall sensitivity was obtained with HF in ELISA (85.5%), followed by IC containing HF and 2B2t-HF (83.0% and 78.2%, respectively). The lowest sensitivity was obtained with B2t and 2B2t in ELISA (51.8%). The highest specificity was obtained with IC containing 2B2t-HF (100%), and the lowest with HF-ELISA (78.0%). The lowest cross-reactivity with sera from patients with alveolar echinococcosis was detected with the recombinant antigens in ELISA (9.5% - 16.7%) and the highest with the HF-IC (64.3%). The results of B2t and 2B2t-ELISA were influenced by cyst stage, as classified by US according to the WHO-Informal Working Group on Echinococcosis (WHO-IWGE), with low sensitivity for inactive (CE4 and CE5) cysts, and by the drug treatment, with higher sensitivity in patients after drug treatment compared with patients not subjected to drug treatment. The two recombinant antigens in ELISA provided promising results for monitoring patients in follow-up, although their use is limited to patients with positive serology against them at the beginning of the followup. Potential biological reasons behind the low sensitivity of the recombinant antigens and possible strategies to enhance the performance of CE serology are discussed. This research received funding from the European Community's Seventh Framework Programme under the grant agreement 602051 (Project HERACLES: Human cystic Echinococcosis ReseArch in CentraL and Eastern Societies; http:// www.heracles-fp7.eu/). The funding body had no involvement in the conception, preparation and writing of the manuscript, in the viewpoints expressed, nor in the decision to submit this article. Peer reviewed 2019-03-27T09:29:06Z 2019-03-27T09:29:06Z 2018 artículo http://purl.org/coar/resource_type/c_6501 PLoS Neglected Tropical Diseases 12(9): e0006741 (2018) 1935-2727 http://hdl.handle.net/10261/178623 10.1371/journal.pntd.0006741 1935-2735 https://doi.org/10.1371/journal.pntd.0006741 http://dx.doi.org/10.13039/501100000780 30188936 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/FP7/602051 Publisher's version https://doi.org/10.1371/journal.pntd.0006741 Sí open Public Library of Science

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