Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs

Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs

12 pages, 4 figures.-- PMID: 17337122 [PubMed].-- Printed version published Apr 30, 2007.

Saved in:
Bibliographic Details
Main Authors: Manzano Román, Raúl, García Varas, Susana, Encinas Grandes, Antonio, Pérez Sánchez, Ricardo
Format: artículo biblioteca
Language:English
Published: Elsevier 2007-03-06
Subjects:Ornithodoros erraticus, Tick, Vaccine, Midgut antigen, Membrane protein, Oe45,
Online Access:http://hdl.handle.net/10261/10331
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-irnasa-es-10261-10331
record_format koha
spelling dig-irnasa-es-10261-103312019-05-09T10:50:38Z Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs Manzano Román, Raúl García Varas, Susana Encinas Grandes, Antonio Pérez Sánchez, Ricardo Ornithodoros erraticus Tick Vaccine Midgut antigen Membrane protein Oe45 12 pages, 4 figures.-- PMID: 17337122 [PubMed].-- Printed version published Apr 30, 2007. Ornithodoros erraticus is an argasid tick that can transmit severe diseases such as human relapsing fever and African swine fever. In the search for a vaccine against this parasite, a crude extract of tick midgut membranes (GME) was obtained that in pigs and mice induced a protective response able to kill up to 80% of the nymphs in the first 72 h post-feeding and to reduce the fecundity of females by more than 50%. To identify the protective antigens, the GME was subjected to successive biochemical fractionations and the resulting simpler protein fractions were inoculated in pigs. A 45-kDa antigen, the so-called Oe45, was detected, purified and demonstrated to be responsible for the protection induced by the GME. Oe45 seems to be a membrane protein that is presumably expressed on the luminal membrane of midgut epithelial cells. Oe45 consists of at least two differently charged bands (cationic and neutral), which show antigenic cross-reactivity. The possibility that these bands might be different isoforms of the same protein is discussed. Although Oe45 is constitutively expressed at low levels throughout the trophogonic cycle, its expression is up-regulated by the ingestion of blood, as suggested by the higher levels observed between 6 and 72 h post-feeding. This work was supported by grant no. AGF99-0798 from the Spanish government and by grant no. QRLT-2000-02216 from the European Commission. Peer reviewed 2009-02-05T10:38:20Z 2009-02-05T10:38:20Z 2007-03-06 artículo http://purl.org/coar/resource_type/c_6501 Veterinary Parasitology 145(3-4): 314-325 (2007) 0304-4017 http://hdl.handle.net/10261/10331 10.1016/j.vetpar.2007.01.011 en http://dx.doi.org/10.1016/j.vetpar.2007.01.011 open 205 bytes text/plain Elsevier
institution IRNASA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-irnasa-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IRNASA España
language English
topic Ornithodoros erraticus
Tick
Vaccine
Midgut antigen
Membrane protein
Oe45
Ornithodoros erraticus
Tick
Vaccine
Midgut antigen
Membrane protein
Oe45
spellingShingle Ornithodoros erraticus
Tick
Vaccine
Midgut antigen
Membrane protein
Oe45
Ornithodoros erraticus
Tick
Vaccine
Midgut antigen
Membrane protein
Oe45
Manzano Román, Raúl
García Varas, Susana
Encinas Grandes, Antonio
Pérez Sánchez, Ricardo
Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs
description 12 pages, 4 figures.-- PMID: 17337122 [PubMed].-- Printed version published Apr 30, 2007.
format artículo
topic_facet Ornithodoros erraticus
Tick
Vaccine
Midgut antigen
Membrane protein
Oe45
author Manzano Román, Raúl
García Varas, Susana
Encinas Grandes, Antonio
Pérez Sánchez, Ricardo
author_facet Manzano Román, Raúl
García Varas, Susana
Encinas Grandes, Antonio
Pérez Sánchez, Ricardo
author_sort Manzano Román, Raúl
title Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs
title_short Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs
title_full Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs
title_fullStr Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs
title_full_unstemmed Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs
title_sort purification and characterization of a 45-kda concealed antigen from the midgut membranes of ornithodoros erraticus that induces lethal anti-tick immune responses in pigs
publisher Elsevier
publishDate 2007-03-06
url http://hdl.handle.net/10261/10331
work_keys_str_mv AT manzanoromanraul purificationandcharacterizationofa45kdaconcealedantigenfromthemidgutmembranesofornithodoroserraticusthatinduceslethalantitickimmuneresponsesinpigs
AT garciavarassusana purificationandcharacterizationofa45kdaconcealedantigenfromthemidgutmembranesofornithodoroserraticusthatinduceslethalantitickimmuneresponsesinpigs
AT encinasgrandesantonio purificationandcharacterizationofa45kdaconcealedantigenfromthemidgutmembranesofornithodoroserraticusthatinduceslethalantitickimmuneresponsesinpigs
AT perezsanchezricardo purificationandcharacterizationofa45kdaconcealedantigenfromthemidgutmembranesofornithodoroserraticusthatinduceslethalantitickimmuneresponsesinpigs
_version_ 1777665187024732160

Similar Items