Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs
12 pages, 4 figures.-- PMID: 17337122 [PubMed].-- Printed version published Apr 30, 2007.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2007-03-06
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Subjects: | Ornithodoros erraticus, Tick, Vaccine, Midgut antigen, Membrane protein, Oe45, |
Online Access: | http://hdl.handle.net/10261/10331 |
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dig-irnasa-es-10261-103312019-05-09T10:50:38Z Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs Manzano Román, Raúl García Varas, Susana Encinas Grandes, Antonio Pérez Sánchez, Ricardo Ornithodoros erraticus Tick Vaccine Midgut antigen Membrane protein Oe45 12 pages, 4 figures.-- PMID: 17337122 [PubMed].-- Printed version published Apr 30, 2007. Ornithodoros erraticus is an argasid tick that can transmit severe diseases such as human relapsing fever and African swine fever. In the search for a vaccine against this parasite, a crude extract of tick midgut membranes (GME) was obtained that in pigs and mice induced a protective response able to kill up to 80% of the nymphs in the first 72 h post-feeding and to reduce the fecundity of females by more than 50%. To identify the protective antigens, the GME was subjected to successive biochemical fractionations and the resulting simpler protein fractions were inoculated in pigs. A 45-kDa antigen, the so-called Oe45, was detected, purified and demonstrated to be responsible for the protection induced by the GME. Oe45 seems to be a membrane protein that is presumably expressed on the luminal membrane of midgut epithelial cells. Oe45 consists of at least two differently charged bands (cationic and neutral), which show antigenic cross-reactivity. The possibility that these bands might be different isoforms of the same protein is discussed. Although Oe45 is constitutively expressed at low levels throughout the trophogonic cycle, its expression is up-regulated by the ingestion of blood, as suggested by the higher levels observed between 6 and 72 h post-feeding. This work was supported by grant no. AGF99-0798 from the Spanish government and by grant no. QRLT-2000-02216 from the European Commission. Peer reviewed 2009-02-05T10:38:20Z 2009-02-05T10:38:20Z 2007-03-06 artículo http://purl.org/coar/resource_type/c_6501 Veterinary Parasitology 145(3-4): 314-325 (2007) 0304-4017 http://hdl.handle.net/10261/10331 10.1016/j.vetpar.2007.01.011 en http://dx.doi.org/10.1016/j.vetpar.2007.01.011 open 205 bytes text/plain Elsevier |
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Ornithodoros erraticus Tick Vaccine Midgut antigen Membrane protein Oe45 Ornithodoros erraticus Tick Vaccine Midgut antigen Membrane protein Oe45 |
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Ornithodoros erraticus Tick Vaccine Midgut antigen Membrane protein Oe45 Ornithodoros erraticus Tick Vaccine Midgut antigen Membrane protein Oe45 Manzano Román, Raúl García Varas, Susana Encinas Grandes, Antonio Pérez Sánchez, Ricardo Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs |
description |
12 pages, 4 figures.-- PMID: 17337122 [PubMed].-- Printed version published Apr 30, 2007. |
format |
artículo |
topic_facet |
Ornithodoros erraticus Tick Vaccine Midgut antigen Membrane protein Oe45 |
author |
Manzano Román, Raúl García Varas, Susana Encinas Grandes, Antonio Pérez Sánchez, Ricardo |
author_facet |
Manzano Román, Raúl García Varas, Susana Encinas Grandes, Antonio Pérez Sánchez, Ricardo |
author_sort |
Manzano Román, Raúl |
title |
Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs |
title_short |
Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs |
title_full |
Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs |
title_fullStr |
Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs |
title_full_unstemmed |
Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs |
title_sort |
purification and characterization of a 45-kda concealed antigen from the midgut membranes of ornithodoros erraticus that induces lethal anti-tick immune responses in pigs |
publisher |
Elsevier |
publishDate |
2007-03-06 |
url |
http://hdl.handle.net/10261/10331 |
work_keys_str_mv |
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