Chemotactic Bacteria Facilitate the Dispersion of Nonmotile Bacteria through Micrometer-Sized Pores in Engineered Porous Media

Chemotactic Bacteria Facilitate the Dispersion of Nonmotile Bacteria through Micrometer-Sized Pores in Engineered Porous Media

10 páginas.- 4 figuras.- 4 tablas.- 45 referencias.- The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.est.2c03149

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Main Authors: Balseiro-Romero, M., Prieto-Fernández, Ángeles, Shor, Leslie M., Ghoshal, S., Baveye, P., Ortega Calvo, J. J.
Other Authors: European Commission
Format: artículo biblioteca
Language:English
Published: American Chemical Society 2022-09
Subjects:Microbe-microbe cotransport, Chemotaxis, Bioaccessibility, Micrometer-sized pores, Hitchhiking,
Online Access:http://hdl.handle.net/10261/281764
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100004837
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spelling dig-irnas-es-10261-2817642022-11-04T17:25:26Z Chemotactic Bacteria Facilitate the Dispersion of Nonmotile Bacteria through Micrometer-Sized Pores in Engineered Porous Media Balseiro-Romero, M. Prieto-Fernández, Ángeles Shor, Leslie M. Ghoshal, S. Baveye, P. Ortega Calvo, J. J. European Commission Ministerio de Ciencia e Innovación (España) Agencia Estatal de Investigación (España) Balseiro-Romero, M. [0000-0003-0831-3899] Prieto-Fernández, Ángeles [0000-0003-2601-1528] Ghoshal, S. [0000-0001-9968-6150] Baveye, P. [0000-0002-8432-6141] Ortega Calvo, J. J. [0000-0003-1672-5199] Microbe-microbe cotransport Chemotaxis Bioaccessibility Micrometer-sized pores Hitchhiking 10 páginas.- 4 figuras.- 4 tablas.- 45 referencias.- The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.est.2c03149 Recent research has demonstrated that chemotactic bacteria can disperse inside microsized pores while traveling toward favorable conditions. Microbe–microbe cotransport might enable nonmotile bacteria to be carried with motile partners to enhance their dispersion and reduce their deposition in porous systems. The aim of this study was to demonstrate the enhancement in the dispersion of nonmotile bacteria (Mycobacterium gilvum VM552, a polycyclic aromatic hydrocarbon-degrader, and Sphingobium sp. D4, a hexachlorocyclohexane-degrader, through micrometer-sized pores near the exclusion-cell-size limit, in the presence of motile Pseudomonas putida G7 cells. For this purpose, we used bioreactors equipped with two chambers that were separated with membrane filters with 3, 5, and 12 μm pore sizes and capillary polydimethylsiloxane (PDMS) microarrays (20 μm × 35 μm × 2.2 mm). The cotransport of nonmotile bacteria occurred exclusively in the presence of a chemoattractant concentration gradient, and therefore, a directed flow of motile cells. This cotransport was more intense in the presence of larger pores (12 μm) and strong chemoeffectors (γ-aminobutyric acid). The mechanism that governed cotransport at the cell scale involved mechanical pushing and hydrodynamic interactions. Chemotaxis-mediated cotransport of bacterial degraders and its implications in pore accessibility opens new avenues for the enhancement of bacterial dispersion in porous media and the biodegradation of heterogeneously contaminated scenarios. This research was funded by European Union's Horizon 2020 research and innovation program under the Marie Sklodowska- Curie grant agreement no. 895340 (BIOTAC project) and by the Spanish Ministry of Science and Innovation (PID2019- 109700RB-C21) . Peer reviewed 2022-10-28T06:33:37Z 2022-10-28T06:33:37Z 2022-09 artículo Environmental Science and Technology - Columbus 56:13975-13984(2022) 0013-936X http://hdl.handle.net/10261/281764 10.1021/acs.est.2c03149A 1520-5851 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/895340 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109700RB-C21/ES/HACIA UNA MODULACION BASADA EN RIESGO DE LOS FLUJOS DE CARBONO IMPLICADOS EN LA ELIMINACION BIOLOGICA DE CONTAMINANTES ORGANICOS EN SUELOS: BIODISPONIBILIDAD/ Publisher's version http://dx.doi.org/10.1021/acs.est.2c03149 Sí open American Chemical Society
institution IRNAS ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-irnas-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IRNAS España
language English
topic Microbe-microbe cotransport
Chemotaxis
Bioaccessibility
Micrometer-sized pores
Hitchhiking
Microbe-microbe cotransport
Chemotaxis
Bioaccessibility
Micrometer-sized pores
Hitchhiking
spellingShingle Microbe-microbe cotransport
Chemotaxis
Bioaccessibility
Micrometer-sized pores
Hitchhiking
Microbe-microbe cotransport
Chemotaxis
Bioaccessibility
Micrometer-sized pores
Hitchhiking
Balseiro-Romero, M.
Prieto-Fernández, Ángeles
Shor, Leslie M.
Ghoshal, S.
Baveye, P.
Ortega Calvo, J. J.
Chemotactic Bacteria Facilitate the Dispersion of Nonmotile Bacteria through Micrometer-Sized Pores in Engineered Porous Media
description 10 páginas.- 4 figuras.- 4 tablas.- 45 referencias.- The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.est.2c03149
author2 European Commission
author_facet European Commission
Balseiro-Romero, M.
Prieto-Fernández, Ángeles
Shor, Leslie M.
Ghoshal, S.
Baveye, P.
Ortega Calvo, J. J.
format artículo
topic_facet Microbe-microbe cotransport
Chemotaxis
Bioaccessibility
Micrometer-sized pores
Hitchhiking
author Balseiro-Romero, M.
Prieto-Fernández, Ángeles
Shor, Leslie M.
Ghoshal, S.
Baveye, P.
Ortega Calvo, J. J.
author_sort Balseiro-Romero, M.
title Chemotactic Bacteria Facilitate the Dispersion of Nonmotile Bacteria through Micrometer-Sized Pores in Engineered Porous Media
title_short Chemotactic Bacteria Facilitate the Dispersion of Nonmotile Bacteria through Micrometer-Sized Pores in Engineered Porous Media
title_full Chemotactic Bacteria Facilitate the Dispersion of Nonmotile Bacteria through Micrometer-Sized Pores in Engineered Porous Media
title_fullStr Chemotactic Bacteria Facilitate the Dispersion of Nonmotile Bacteria through Micrometer-Sized Pores in Engineered Porous Media
title_full_unstemmed Chemotactic Bacteria Facilitate the Dispersion of Nonmotile Bacteria through Micrometer-Sized Pores in Engineered Porous Media
title_sort chemotactic bacteria facilitate the dispersion of nonmotile bacteria through micrometer-sized pores in engineered porous media
publisher American Chemical Society
publishDate 2022-09
url http://hdl.handle.net/10261/281764
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100004837
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