Engineering Collariella virescens Peroxygenase for Epoxides Production from Vegetable Oil

Engineering Collariella virescens Peroxygenase for Epoxides Production from Vegetable Oil

17 páginas.- 4 figuras.- 4 tablas.- 46 referencias.- Supplementary Materials: The following supporting information can be downloaded at https://www.mdpi.com/article/10.3390/antiox11050915/s1

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Bibliographic Details
Main Authors: Linde, Dolores, González-Benjumea, Alejandro, Aranda, Carmen, Carro, Juan, Gutiérrez Suárez, Ana, Martínez, Ángel T.
Other Authors: Circular Bio-based Europe Joint Undertaking
Format: artículo biblioteca
Language:English
Published: Multidisciplinary Digital Publishing Institute 2022-05-06
Subjects:Collariella virescens, Epoxidation, heme access channel, Process optimization, Protein engineering, Sunflower oil, Unsaturated fatty acids, Unspecific peroxygenase (UPO),
Online Access:http://hdl.handle.net/10261/270706
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100000780
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record_format koha
institution IRNAS ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-irnas-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IRNAS España
language English
topic Collariella virescens
Epoxidation
heme access channel
Process optimization
Protein engineering
Sunflower oil
Unsaturated fatty acids
Unspecific peroxygenase (UPO)
Collariella virescens
Epoxidation
heme access channel
Process optimization
Protein engineering
Sunflower oil
Unsaturated fatty acids
Unspecific peroxygenase (UPO)
spellingShingle Collariella virescens
Epoxidation
heme access channel
Process optimization
Protein engineering
Sunflower oil
Unsaturated fatty acids
Unspecific peroxygenase (UPO)
Collariella virescens
Epoxidation
heme access channel
Process optimization
Protein engineering
Sunflower oil
Unsaturated fatty acids
Unspecific peroxygenase (UPO)
Linde, Dolores
González-Benjumea, Alejandro
Aranda, Carmen
Carro, Juan
Gutiérrez Suárez, Ana
Martínez, Ángel T.
Engineering Collariella virescens Peroxygenase for Epoxides Production from Vegetable Oil
description 17 páginas.- 4 figuras.- 4 tablas.- 46 referencias.- Supplementary Materials: The following supporting information can be downloaded at https://www.mdpi.com/article/10.3390/antiox11050915/s1
author2 Circular Bio-based Europe Joint Undertaking
author_facet Circular Bio-based Europe Joint Undertaking
Linde, Dolores
González-Benjumea, Alejandro
Aranda, Carmen
Carro, Juan
Gutiérrez Suárez, Ana
Martínez, Ángel T.
format artículo
topic_facet Collariella virescens
Epoxidation
heme access channel
Process optimization
Protein engineering
Sunflower oil
Unsaturated fatty acids
Unspecific peroxygenase (UPO)
author Linde, Dolores
González-Benjumea, Alejandro
Aranda, Carmen
Carro, Juan
Gutiérrez Suárez, Ana
Martínez, Ángel T.
author_sort Linde, Dolores
title Engineering Collariella virescens Peroxygenase for Epoxides Production from Vegetable Oil
title_short Engineering Collariella virescens Peroxygenase for Epoxides Production from Vegetable Oil
title_full Engineering Collariella virescens Peroxygenase for Epoxides Production from Vegetable Oil
title_fullStr Engineering Collariella virescens Peroxygenase for Epoxides Production from Vegetable Oil
title_full_unstemmed Engineering Collariella virescens Peroxygenase for Epoxides Production from Vegetable Oil
title_sort engineering collariella virescens peroxygenase for epoxides production from vegetable oil
publisher Multidisciplinary Digital Publishing Institute
publishDate 2022-05-06
url http://hdl.handle.net/10261/270706
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100000780
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spelling dig-irnas-es-10261-2707062022-10-05T07:31:53Z Engineering Collariella virescens Peroxygenase for Epoxides Production from Vegetable Oil Linde, Dolores González-Benjumea, Alejandro Aranda, Carmen Carro, Juan Gutiérrez Suárez, Ana Martínez, Ángel T. Circular Bio-based Europe Joint Undertaking European Commission Ministerio de Ciencia e Innovación (España) Agencia Estatal de Investigación (España) Consejo Superior de Investigaciones Científicas (España) Linde, Dolores [0000-0002-0359-0566] González-Benjumea, Alejandro [0000-0003-2857-9491] Aranda, Carmen [0000-0001-8213-1132] Carro, Juan [0000-0002-7556-9782] Gutiérrez Suárez, Ana [0000-0002-8823-9029] Martínez, Ángel T. [0000-0002-1584-2863] Collariella virescens Epoxidation heme access channel Process optimization Protein engineering Sunflower oil Unsaturated fatty acids Unspecific peroxygenase (UPO) 17 páginas.- 4 figuras.- 4 tablas.- 46 referencias.- Supplementary Materials: The following supporting information can be downloaded at https://www.mdpi.com/article/10.3390/antiox11050915/s1 Vegetable oils are valuable renewable resources for the production of bio-based chemicals and intermediates, including reactive epoxides of industrial interest. Enzymes are an environmentally friendly alternative to chemical catalysis in oxygenation reactions, epoxidation included, with the added advantage of their potential selectivity. The unspecific peroxygenase of Collariella virescens is only available as a recombinant enzyme (rCviUPO), which is produced in Escherichia coli for protein engineering and analytical-scale optimization of plant lipid oxygenation. Engineering the active site of rCviUPO (by substituting one, two, or up to six residues of its access channel by alanines) improved the epoxidation of individual 18-C unsaturated fatty acids and hydrolyzed sunflower oil. The double mutation at the heme channel (F88A/T158A) enhanced epoxidation of polyunsaturated linoleic and α–linolenic acids, with the desired diepoxides representing > 80% of the products (after 99% substrate conversion). More interestingly, process optimization increased (by 100-fold) the hydrolyzate concentration, with up to 85% epoxidation yield, after 1 h of reaction time with the above double variant. Under these conditions, oleic acid monoepoxide and linoleic acid diepoxide are the main products from the sunflower oil hydrolyzate. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This research was funded by BioBased Industries Joint Undertaking under the European Unions Horizon 2020 Research and Innovation Programme, grant number 792063 (SusBind project; https://susbind.eu; to A.G and A.T.M.); the BIO2017-86559-R project of the Spanish Ministry of Science and Innovation (co-financed by FEDER funds; to A.T.M.); the PID2020-118968RB-I00 project by the Spanish MCIN/AEI/10.13039/501100011033 (to A.G.); the CSIC projects PIE-202040E185 (to A.G.) and PIE-202120E019 (to A.T.M.); the CSIC SusPlast platform (to A.T.M.); and the CSIC program for the Spanish Recovery, Transformation, and Resilience Plan funded by the Recovery and Resilience Facility of the European Union, established by the Regulation (EU) 2020/2094. Peer reviewed 2022-05-27T07:40:15Z 2022-05-27T07:40:15Z 2022-05-06 artículo http://purl.org/coar/resource_type/c_6501 Antioxidants 11(5): 915 (2022) 2076-3921 http://hdl.handle.net/10261/270706 10.3390/antiox11050915 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100000780 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/792063 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-86559-R/ES/GENOMAS DE BASIDIOMICETOS PARA LAS BIORREFINERIAS DE LIGNOCELULOSA/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-118968RB-I00/ES/LIGNINAS Y LIPIDOS: FUENTES RENOVABLES DE NUEVOS BIOPRODUCTOS/ Publisher's version http://dx.doi.org/10.3390/antiox11050915 Sí open Multidisciplinary Digital Publishing Institute

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