Molecular determinants for selective C25-hydroxylation of vitamins D2 and D3 by fungal peroxygenases
Poster nº 7 presentado en el 4th New Trends in Computational Chemistry for Industrial Applications 1-2 Octubre, Barcelona (2015)
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Universidad de Barcelona
2015-10-01
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dig-irnas-es-10261-1332172020-11-13T13:29:43Z Molecular determinants for selective C25-hydroxylation of vitamins D2 and D3 by fungal peroxygenases Cañellas, Marina Lucas, Fátima Babot, Esteban Daniel Río Andrade, José Carlos del Kalum, Lisbeth Ullrich, René Hofrichter, Martin Guallar, Victor Martínez, Ángel T. Gutiérrez Suárez, Ana Poster nº 7 presentado en el 4th New Trends in Computational Chemistry for Industrial Applications 1-2 Octubre, Barcelona (2015) Selective oxygenations of aliphatic compounds are among the most challenging reactions in organic chemistry for the regio and/or stereo specific synthesis of pharmaceuticals and fine chemicals. Recently, a new peroxidase type, which shares the active-site architecture and reaction mechanism of cytochromes P450, but has the advantage of being activated directly by H2O2, was isolated from Agrocybe aegerita (Ullrich, 2004), and later identified in a variety of sequenced basidiomycete genomes, including that of Coprinopsis cinerea (Floudas, 2012). The A. aegerita heme-thiolate (HTP) enzyme has been the most widely investigated basidiomycete peroxygenase, and recent studies have shown that the C. cinerea enzyme has comparative advantages related to its high conversion yield/selectivity for some hydroxylation reactions, and its production as a recombinant protein in an industrial expression host (Babot, 2013; 2014). 25-monohydroxylated vitamin D3 (cholecalciferol) and D2 (ergocalciferol), compounds of high interest in human health and animal feeding, can be obtained through reaction with both A. aegerita and C. cinerea enzymes. In the present study, PELE computational analyses are used to rationalize the underlying mechanisms responsible for differences in reactivity and selectivity observed experimentally between both enzymes (Lucas, 2015). References Babot, E. D., et al., Biotechnol. Bioeng., (2013), 110, 2332. Babot, E. D., et al., Chemcatchem, (2014), online. Floudas, D., et al. Science, (2012), 336, 1715-1719. Lucas, F., et al. Catal. Sci. Tech., (2015), online. Ullrich, R., et al., Appl. Environ. Microbiol. (2004), 70, 4575-4581. Peer reviewed 2016-06-09T09:48:08Z 2016-06-09T09:48:08Z 2015-10-01 póster de congreso http://purl.org/coar/resource_type/c_6670 4th New Trends in Computational Chemistry for Industrial Applications Póster 7 (2015) http://hdl.handle.net/10261/133217 en http://www.xrqtc.com/workshop2015/wp-content/uploads/sites/2/2015/05/POSTER-SESSION.pdf Sí none Universidad de Barcelona |
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Poster nº 7 presentado en el 4th New Trends in Computational Chemistry for Industrial Applications 1-2 Octubre, Barcelona (2015) |
| format |
póster de congreso |
| author |
Cañellas, Marina Lucas, Fátima Babot, Esteban Daniel Río Andrade, José Carlos del Kalum, Lisbeth Ullrich, René Hofrichter, Martin Guallar, Victor Martínez, Ángel T. Gutiérrez Suárez, Ana |
| spellingShingle |
Cañellas, Marina Lucas, Fátima Babot, Esteban Daniel Río Andrade, José Carlos del Kalum, Lisbeth Ullrich, René Hofrichter, Martin Guallar, Victor Martínez, Ángel T. Gutiérrez Suárez, Ana Molecular determinants for selective C25-hydroxylation of vitamins D2 and D3 by fungal peroxygenases |
| author_facet |
Cañellas, Marina Lucas, Fátima Babot, Esteban Daniel Río Andrade, José Carlos del Kalum, Lisbeth Ullrich, René Hofrichter, Martin Guallar, Victor Martínez, Ángel T. Gutiérrez Suárez, Ana |
| author_sort |
Cañellas, Marina |
| title |
Molecular determinants for selective C25-hydroxylation of vitamins D2 and D3 by fungal peroxygenases |
| title_short |
Molecular determinants for selective C25-hydroxylation of vitamins D2 and D3 by fungal peroxygenases |
| title_full |
Molecular determinants for selective C25-hydroxylation of vitamins D2 and D3 by fungal peroxygenases |
| title_fullStr |
Molecular determinants for selective C25-hydroxylation of vitamins D2 and D3 by fungal peroxygenases |
| title_full_unstemmed |
Molecular determinants for selective C25-hydroxylation of vitamins D2 and D3 by fungal peroxygenases |
| title_sort |
molecular determinants for selective c25-hydroxylation of vitamins d2 and d3 by fungal peroxygenases |
| publisher |
Universidad de Barcelona |
| publishDate |
2015-10-01 |
| url |
http://hdl.handle.net/10261/133217 |
| work_keys_str_mv |
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