In vivo analysis of the stability and fitness of variants recovered from foot-and-mouth disease virus quasispecies
We have analysed the ability to infect pigs of two foot-and-mouth disease virus (FMDV) variants isolated at low frequencies from virus populations (quasispecies) generated in pigs on infection with a parental virus, C-S8c1. A monoclonal antibody-resistant mutant (MARM21), and a variant isolated at early times post-infection (S-3T1), each exhibiting a unique amino acid substitution in VP1, were able to cause disease in pigs, both by direct inoculation or by contact transmission. The symptoms developed were similar to those produced by C-S8c1 or the related virus C-S15c1. The VP1 sequence of viral RNA directly recovered from lesions of infected animals confirmed the stability of the variant genotypes. Pigs infected with S-3T1 consistently showed an advance of 12 to 24 h in the emergence of fever and lesions when compared to animals infected with C-S8c1 or the remaining variants, an observation consistent with its early isolation. The ability of FMDV variants to compete in vivo with C-S8c1 was investigated in co-infection experiments. Analysis of the proportion of each of the competitors in lesions of co-infected pigs revealed that none of the variants was completely overgrown by the parent. However, co-infection with C-S8c1 and MARM21 resulted in lesions in which C-S8c1 was predominant, indicating a selective disadvantage of this variant in swine. In contrast, lesions from swine coinfected with C-S8c1 and S-3T1 contained similar proportions of the two viruses. These results document fitness variations in vivo among components of the mutant spectrum of FMDV quasispecies.
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1998
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dig-inia-es-20.500.12792-57692020-12-15T09:55:05Z In vivo analysis of the stability and fitness of variants recovered from foot-and-mouth disease virus quasispecies Carrillo, C. Borca, M. Moore, D. M. Morgan, D. O. Sobrino, F. We have analysed the ability to infect pigs of two foot-and-mouth disease virus (FMDV) variants isolated at low frequencies from virus populations (quasispecies) generated in pigs on infection with a parental virus, C-S8c1. A monoclonal antibody-resistant mutant (MARM21), and a variant isolated at early times post-infection (S-3T1), each exhibiting a unique amino acid substitution in VP1, were able to cause disease in pigs, both by direct inoculation or by contact transmission. The symptoms developed were similar to those produced by C-S8c1 or the related virus C-S15c1. The VP1 sequence of viral RNA directly recovered from lesions of infected animals confirmed the stability of the variant genotypes. Pigs infected with S-3T1 consistently showed an advance of 12 to 24 h in the emergence of fever and lesions when compared to animals infected with C-S8c1 or the remaining variants, an observation consistent with its early isolation. The ability of FMDV variants to compete in vivo with C-S8c1 was investigated in co-infection experiments. Analysis of the proportion of each of the competitors in lesions of co-infected pigs revealed that none of the variants was completely overgrown by the parent. However, co-infection with C-S8c1 and MARM21 resulted in lesions in which C-S8c1 was predominant, indicating a selective disadvantage of this variant in swine. In contrast, lesions from swine coinfected with C-S8c1 and S-3T1 contained similar proportions of the two viruses. These results document fitness variations in vivo among components of the mutant spectrum of FMDV quasispecies. 2020-10-22T21:11:17Z 2020-10-22T21:11:17Z 1998 journal article http://hdl.handle.net/20.500.12792/5769 10.1099/0022-1317-79-7-1699 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
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We have analysed the ability to infect pigs of two foot-and-mouth disease virus (FMDV) variants isolated at low frequencies from virus populations (quasispecies) generated in pigs on infection with a parental virus, C-S8c1. A monoclonal antibody-resistant mutant (MARM21), and a variant isolated at early times post-infection (S-3T1), each exhibiting a unique amino acid substitution in VP1, were able to cause disease in pigs, both by direct inoculation or by contact transmission. The symptoms developed were similar to those produced by C-S8c1 or the related virus C-S15c1. The VP1 sequence of viral RNA directly recovered from lesions of infected animals confirmed the stability of the variant genotypes. Pigs infected with S-3T1 consistently showed an advance of 12 to 24 h in the emergence of fever and lesions when compared to animals infected with C-S8c1 or the remaining variants, an observation consistent with its early isolation. The ability of FMDV variants to compete in vivo with C-S8c1 was investigated in co-infection experiments. Analysis of the proportion of each of the competitors in lesions of co-infected pigs revealed that none of the variants was completely overgrown by the parent. However, co-infection with C-S8c1 and MARM21 resulted in lesions in which C-S8c1 was predominant, indicating a selective disadvantage of this variant in swine. In contrast, lesions from swine coinfected with C-S8c1 and S-3T1 contained similar proportions of the two viruses. These results document fitness variations in vivo among components of the mutant spectrum of FMDV quasispecies. |
| format |
journal article |
| author |
Carrillo, C. Borca, M. Moore, D. M. Morgan, D. O. Sobrino, F. |
| spellingShingle |
Carrillo, C. Borca, M. Moore, D. M. Morgan, D. O. Sobrino, F. In vivo analysis of the stability and fitness of variants recovered from foot-and-mouth disease virus quasispecies |
| author_facet |
Carrillo, C. Borca, M. Moore, D. M. Morgan, D. O. Sobrino, F. |
| author_sort |
Carrillo, C. |
| title |
In vivo analysis of the stability and fitness of variants recovered from foot-and-mouth disease virus quasispecies |
| title_short |
In vivo analysis of the stability and fitness of variants recovered from foot-and-mouth disease virus quasispecies |
| title_full |
In vivo analysis of the stability and fitness of variants recovered from foot-and-mouth disease virus quasispecies |
| title_fullStr |
In vivo analysis of the stability and fitness of variants recovered from foot-and-mouth disease virus quasispecies |
| title_full_unstemmed |
In vivo analysis of the stability and fitness of variants recovered from foot-and-mouth disease virus quasispecies |
| title_sort |
in vivo analysis of the stability and fitness of variants recovered from foot-and-mouth disease virus quasispecies |
| publishDate |
1998 |
| url |
http://hdl.handle.net/20.500.12792/5769 |
| work_keys_str_mv |
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