Analysis of monoclonal antibodies reactive with the porcine CD4 antigen
As result of the First International Swine CD Workshop, four monoclonal antibodies (mAb) (#002, 054, 118, and 127) clustered closely to the internal standard anti-porcine CD4 mAb, 74-12-4. To further characterize the relationship between these mAb, they were used in flow cytometry to inhibit binding of 74-12-4 to porcine lymphocytes. mAb #002 (74-12-4) and #054 (PT90) completely inhibited, while mAb #127 (10.2H2) and #118 (b38c6) partially inhibited (57% and 77% respectively), the binding of 74-12-4. Furthermore, none of the mAbs bound to a 74-12-4 negative strain of pigs. We conclude that the four mAb bind to the same, or a closely related, epitope on porcine lymphocytes. © 1994.
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dig-inia-es-20.500.12792-34892020-12-15T09:52:43Z Analysis of monoclonal antibodies reactive with the porcine CD4 antigen Pescovitz, M. D. Aasted, B. Canals, A. Dominguez, J. Sanchez Vizcaino, J. M. Pospisil, R. Trebichavsky, I. Salmon, H. Valpotic, I. Davis, W. C. Arn, S. Sachs, D. H. Lunney, J. K. Zuckerman, F. Weiland, E. Saalmueller, A. As result of the First International Swine CD Workshop, four monoclonal antibodies (mAb) (#002, 054, 118, and 127) clustered closely to the internal standard anti-porcine CD4 mAb, 74-12-4. To further characterize the relationship between these mAb, they were used in flow cytometry to inhibit binding of 74-12-4 to porcine lymphocytes. mAb #002 (74-12-4) and #054 (PT90) completely inhibited, while mAb #127 (10.2H2) and #118 (b38c6) partially inhibited (57% and 77% respectively), the binding of 74-12-4. Furthermore, none of the mAbs bound to a 74-12-4 negative strain of pigs. We conclude that the four mAb bind to the same, or a closely related, epitope on porcine lymphocytes. © 1994. 2020-10-22T14:47:16Z 2020-10-22T14:47:16Z 1994 journal article http://hdl.handle.net/20.500.12792/3489 10.1016/0165-2427(94)90141-4 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
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As result of the First International Swine CD Workshop, four monoclonal antibodies (mAb) (#002, 054, 118, and 127) clustered closely to the internal standard anti-porcine CD4 mAb, 74-12-4. To further characterize the relationship between these mAb, they were used in flow cytometry to inhibit binding of 74-12-4 to porcine lymphocytes. mAb #002 (74-12-4) and #054 (PT90) completely inhibited, while mAb #127 (10.2H2) and #118 (b38c6) partially inhibited (57% and 77% respectively), the binding of 74-12-4. Furthermore, none of the mAbs bound to a 74-12-4 negative strain of pigs. We conclude that the four mAb bind to the same, or a closely related, epitope on porcine lymphocytes. © 1994. |
| format |
journal article |
| author |
Pescovitz, M. D. Aasted, B. Canals, A. Dominguez, J. Sanchez Vizcaino, J. M. Pospisil, R. Trebichavsky, I. Salmon, H. Valpotic, I. Davis, W. C. Arn, S. Sachs, D. H. Lunney, J. K. Zuckerman, F. Weiland, E. Saalmueller, A. |
| spellingShingle |
Pescovitz, M. D. Aasted, B. Canals, A. Dominguez, J. Sanchez Vizcaino, J. M. Pospisil, R. Trebichavsky, I. Salmon, H. Valpotic, I. Davis, W. C. Arn, S. Sachs, D. H. Lunney, J. K. Zuckerman, F. Weiland, E. Saalmueller, A. Analysis of monoclonal antibodies reactive with the porcine CD4 antigen |
| author_facet |
Pescovitz, M. D. Aasted, B. Canals, A. Dominguez, J. Sanchez Vizcaino, J. M. Pospisil, R. Trebichavsky, I. Salmon, H. Valpotic, I. Davis, W. C. Arn, S. Sachs, D. H. Lunney, J. K. Zuckerman, F. Weiland, E. Saalmueller, A. |
| author_sort |
Pescovitz, M. D. |
| title |
Analysis of monoclonal antibodies reactive with the porcine CD4 antigen |
| title_short |
Analysis of monoclonal antibodies reactive with the porcine CD4 antigen |
| title_full |
Analysis of monoclonal antibodies reactive with the porcine CD4 antigen |
| title_fullStr |
Analysis of monoclonal antibodies reactive with the porcine CD4 antigen |
| title_full_unstemmed |
Analysis of monoclonal antibodies reactive with the porcine CD4 antigen |
| title_sort |
analysis of monoclonal antibodies reactive with the porcine cd4 antigen |
| publishDate |
1994 |
| url |
http://hdl.handle.net/20.500.12792/3489 |
| work_keys_str_mv |
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| _version_ |
1758004601850691584 |