Evaluation of molecular techniques for the genetic characterization of Saccharomyces cerevisiae strains
The applicability of different PCR-based techniques, random amplified polymorphic DNA, cleaved amplified polymorphic sequence and SSRs (simple sequence repeats) to genetically identify, typify and discriminate among Saccharomyces cerevisiae strains, was investigated. The analysis and comparison of the genetic profiles obtained for each of the techniques allowed determination of the degree of polymorphism, genetic diversity and relationships among the strains. Consequently, the discriminatory power and usefulness of the molecular techniques assayed was established. SSR analysis was the most effective method due to its high level of polymorphism, the ease of interpretation of the genetic profiles obtained, and the speed of use. © 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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dig-inia-es-20.500.12792-34012020-12-15T09:54:30Z Evaluation of molecular techniques for the genetic characterization of Saccharomyces cerevisiae strains Pérez, M. A. Gallego, F. J. Hidalgo, P. The applicability of different PCR-based techniques, random amplified polymorphic DNA, cleaved amplified polymorphic sequence and SSRs (simple sequence repeats) to genetically identify, typify and discriminate among Saccharomyces cerevisiae strains, was investigated. The analysis and comparison of the genetic profiles obtained for each of the techniques allowed determination of the degree of polymorphism, genetic diversity and relationships among the strains. Consequently, the discriminatory power and usefulness of the molecular techniques assayed was established. SSR analysis was the most effective method due to its high level of polymorphism, the ease of interpretation of the genetic profiles obtained, and the speed of use. © 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. 2020-10-22T14:36:39Z 2020-10-22T14:36:39Z 2001 journal article http://hdl.handle.net/20.500.12792/3401 10.1016/S0378-1097(01)00511-0 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
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The applicability of different PCR-based techniques, random amplified polymorphic DNA, cleaved amplified polymorphic sequence and SSRs (simple sequence repeats) to genetically identify, typify and discriminate among Saccharomyces cerevisiae strains, was investigated. The analysis and comparison of the genetic profiles obtained for each of the techniques allowed determination of the degree of polymorphism, genetic diversity and relationships among the strains. Consequently, the discriminatory power and usefulness of the molecular techniques assayed was established. SSR analysis was the most effective method due to its high level of polymorphism, the ease of interpretation of the genetic profiles obtained, and the speed of use. © 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. |
format |
journal article |
author |
Pérez, M. A. Gallego, F. J. Hidalgo, P. |
spellingShingle |
Pérez, M. A. Gallego, F. J. Hidalgo, P. Evaluation of molecular techniques for the genetic characterization of Saccharomyces cerevisiae strains |
author_facet |
Pérez, M. A. Gallego, F. J. Hidalgo, P. |
author_sort |
Pérez, M. A. |
title |
Evaluation of molecular techniques for the genetic characterization of Saccharomyces cerevisiae strains |
title_short |
Evaluation of molecular techniques for the genetic characterization of Saccharomyces cerevisiae strains |
title_full |
Evaluation of molecular techniques for the genetic characterization of Saccharomyces cerevisiae strains |
title_fullStr |
Evaluation of molecular techniques for the genetic characterization of Saccharomyces cerevisiae strains |
title_full_unstemmed |
Evaluation of molecular techniques for the genetic characterization of Saccharomyces cerevisiae strains |
title_sort |
evaluation of molecular techniques for the genetic characterization of saccharomyces cerevisiae strains |
publishDate |
2001 |
url |
http://hdl.handle.net/20.500.12792/3401 |
work_keys_str_mv |
AT perezma evaluationofmoleculartechniquesforthegeneticcharacterizationofsaccharomycescerevisiaestrains AT gallegofj evaluationofmoleculartechniquesforthegeneticcharacterizationofsaccharomycescerevisiaestrains AT hidalgop evaluationofmoleculartechniquesforthegeneticcharacterizationofsaccharomycescerevisiaestrains |
_version_ |
1758004800366051328 |