Seasonal variation in sperm freezability associated with changes in testicular germinal epithelium in domestic (Ovis aries) and wild (Ovis musimon) sheep
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CSIRO Publishing
2019-06-11
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| Subjects: | Cryopreservation, Sertoli cell, Spermatogenesis, Spermatozoa, Testis, Testosterone, |
| Online Access: | http://hdl.handle.net/10261/371071 https://api.elsevier.com/content/abstract/scopus_id/85067353198 |
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dig-inia-es-10261-3710712024-11-06T21:37:51Z Seasonal variation in sperm freezability associated with changes in testicular germinal epithelium in domestic (Ovis aries) and wild (Ovis musimon) sheep Martínez-Fresneda, Lucía O'Brien, Emma Velázquez, Rosario Toledano-Díaz, A. Martínez-Cáceres, Carlos Tesfaye, Dawit Schellander, Karl García-Vázquez, Francisco Alberto Santiago Moreno, Julián European Commission Ministerio de Economía y Competitividad (España) Agencia Estatal de Investigación (España) Martínez-Fresneda, Lucía [0000-0002-0967-8593] Velázquez, Rosario [0009-0009-2115-4715] Toledano-Díaz, A. [0000-0001-6679-485X] Tesfaye, Dawit [0000-0001-8166-0606] García-Vázquez, Francisco Alberto [0000-0001-7665-3858] Santiago Moreno, Julián [0000-0001-5551-8120] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] Cryopreservation Sertoli cell Spermatogenesis Spermatozoa Testis Testosterone 13 Pág. The aim of this study was to examine ovine sperm cryoresistance during the rutting season (RS) and its association with sperm head area and seminiferous epithelium proliferation. Small ruminants show fluctuating testosterone levels throughout the year, which could interfere with spermatogenesis and sperm cryopreservation. Ejaculates, testicular biopsies and blood were collected during the middle and at the end of the RS (Middle-RS vs End-RS) during periods of high and low testosterone levels in Merino and Mouflon rams. Fresh and frozen-thawed sperm quality, sperm morphometry, seminiferous tubule morphometry and testicular proliferation markers (proliferating cell nuclear antigen, proliferation marker protein Ki-67 and transcription factor GATA-4) were evaluated. Post-thaw sperm viability was higher in the End-RS group in both Merino (69.9±8.2 vs 41.6±7.3%; P=0.020) and Mouflon rams (40.9±3.3 vs 24.2±5.0%; P=0.008). Mouflons had larger sperm head area at the End-RS (38.3±0.2 vs 34.3±0.1µm2; P=0.029), whereas there was no difference between Merino groups (35.7±0.5 vs 34.8±1.0µm2). Seminiferous tubule morphometry and proliferation markers showed higher levels of germinal epithelium proliferation in the Middle-RS of both species. In conclusion, sperm freezability is affected during the RS in domestic and wild rams, which could be correlated with changes that occur during spermatogenesis, since there is an effect of season on cell proliferation in the testis. This work was supported by European Union Horizon 2020 Marie Sklodowska-Curie Action, REPBIOTECH 675526 and by the Spanish ‘Ministerio de Economıa, Industria y Competitividad’ MINECO AGL2014–52081-R and AGL2017–85753-R. The authors thank Professor Octavio Lopez Albors for his collaboration in the processing of histological sections at the Department of Anatomy and Comparative Pathology (Veterinary Faculty, University of Murcia, Spain) and Dennis Miskel for copyediting Peer reviewed 2024-11-06T07:23:57Z 2024-11-06T07:23:57Z 2019-06-11 artículo http://purl.org/coar/resource_type/c_6501 Publisher's version Reproduction, Fertility and Development 31(10): 1545-1557 (2019) 1031-3613 http://hdl.handle.net/10261/371071 10.1071/RD18511 1448-5990 31182186 2-s2.0-85067353198 https://api.elsevier.com/content/abstract/scopus_id/85067353198 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/675526 info:eu-repo/grantAgreement/MINECO//AGL2014-52081-R/ES/INFLUENCIA DEL ESTATUS ENDOCRINO EN LA RESPUESTA A LA CONGELACION%2FVITRIFICACION DE ESPERMATOZOIDES DE PEQUEÑOS RUMIANTES:USO DE RUMIANTES SILVESTRES%2FDOMESTICOS COMO MODELO/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-85753-R/ES/VARIACIONES EN LA EFICACIA DE LA CRIOPRESERVACION DE ESPERMATOZOIDES DE PEQUEÑOS RUMIANTES COMO RESPUESTA A LOS CAMBIOS DE HORMONAS TIROIDEAS Y LA EXPRESION DE ACUAPORINAS/ Departamento de Reproducción Animal publisher https://doi.org/10.1071/RD18511 Sí open CSIRO Publishing |
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Cryopreservation Sertoli cell Spermatogenesis Spermatozoa Testis Testosterone Cryopreservation Sertoli cell Spermatogenesis Spermatozoa Testis Testosterone |
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Cryopreservation Sertoli cell Spermatogenesis Spermatozoa Testis Testosterone Cryopreservation Sertoli cell Spermatogenesis Spermatozoa Testis Testosterone Martínez-Fresneda, Lucía O'Brien, Emma Velázquez, Rosario Toledano-Díaz, A. Martínez-Cáceres, Carlos Tesfaye, Dawit Schellander, Karl García-Vázquez, Francisco Alberto Santiago Moreno, Julián Seasonal variation in sperm freezability associated with changes in testicular germinal epithelium in domestic (Ovis aries) and wild (Ovis musimon) sheep |
| description |
13 Pág. |
| author2 |
European Commission |
| author_facet |
European Commission Martínez-Fresneda, Lucía O'Brien, Emma Velázquez, Rosario Toledano-Díaz, A. Martínez-Cáceres, Carlos Tesfaye, Dawit Schellander, Karl García-Vázquez, Francisco Alberto Santiago Moreno, Julián |
| format |
artículo |
| topic_facet |
Cryopreservation Sertoli cell Spermatogenesis Spermatozoa Testis Testosterone |
| author |
Martínez-Fresneda, Lucía O'Brien, Emma Velázquez, Rosario Toledano-Díaz, A. Martínez-Cáceres, Carlos Tesfaye, Dawit Schellander, Karl García-Vázquez, Francisco Alberto Santiago Moreno, Julián |
| author_sort |
Martínez-Fresneda, Lucía |
| title |
Seasonal variation in sperm freezability associated with changes in testicular germinal epithelium in domestic (Ovis aries) and wild (Ovis musimon) sheep |
| title_short |
Seasonal variation in sperm freezability associated with changes in testicular germinal epithelium in domestic (Ovis aries) and wild (Ovis musimon) sheep |
| title_full |
Seasonal variation in sperm freezability associated with changes in testicular germinal epithelium in domestic (Ovis aries) and wild (Ovis musimon) sheep |
| title_fullStr |
Seasonal variation in sperm freezability associated with changes in testicular germinal epithelium in domestic (Ovis aries) and wild (Ovis musimon) sheep |
| title_full_unstemmed |
Seasonal variation in sperm freezability associated with changes in testicular germinal epithelium in domestic (Ovis aries) and wild (Ovis musimon) sheep |
| title_sort |
seasonal variation in sperm freezability associated with changes in testicular germinal epithelium in domestic (ovis aries) and wild (ovis musimon) sheep |
| publisher |
CSIRO Publishing |
| publishDate |
2019-06-11 |
| url |
http://hdl.handle.net/10261/371071 https://api.elsevier.com/content/abstract/scopus_id/85067353198 |
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