Thermal and operational deactivation of Aspergillus fumigatus β-glucosidase in ethanol/water pretreated wheat straw enzymatic hydrolysis

Thermal and operational deactivation of Aspergillus fumigatus β-glucosidase in ethanol/water pretreated wheat straw enzymatic hydrolysis

7 Pág.

Saved in:
Bibliographic Details
Main Authors: Wojtusik, M., Vergara, Priscilla, Villar, J. C., García-Ochoa, Félix, Ladero, Miguel
Other Authors: Ministerio de Economía y Competitividad (España)
Format: artículo biblioteca
Language:English
Published: Elsevier 2019-02-20
Subjects:Cellobiose, Saccharification, Thermal stability, Wheat straw, β-glucosidase,
Online Access:http://hdl.handle.net/10261/366105
http://dx.doi.org/10.13039/501100003329
https://api.elsevier.com/content/abstract/scopus_id/85060314099
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-inia-es-10261-366105
record_format koha
spelling dig-inia-es-10261-3661052024-10-27T21:49:19Z Thermal and operational deactivation of Aspergillus fumigatus β-glucosidase in ethanol/water pretreated wheat straw enzymatic hydrolysis Wojtusik, M. Vergara, Priscilla Villar, J. C. García-Ochoa, Félix Ladero, Miguel Ministerio de Economía y Competitividad (España) Wojtusik, M. [0000-0002-2356-463X] Vergara, Priscilla [0000-0003-2876-649X] Villar, J. C. [0000-0001-6850-9792] García-Ochoa, Félix [0000-0002-0921-5900] Ladero, Miguel [0000-0002-9146-3830] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] Cellobiose Saccharification Thermal stability Wheat straw β-glucosidase 7 Pág. The stabilization effects on a novel commercial β-glucosidase preparation from Aspergillus fumigatus during saccharification of ethanol-water pretreated wheat straw were analysed in comparison to this enzyme stability during cellobiose hydrolysis. For this purpose, the kinetics of β-glucosidase residual activity during cellobiose hydrolysis from 40 till 70 °C were studied, resulting in the fitting of a first-order partial deactivation model. Furthermore, a subsequent fitting of a kinetic model including this first-order deactivation equation and a Michaelis-Menten equation with double competitive inhibition by glucose and uncompetitive inhibition by cellobiose to released glucose was successful. Finally, global enzyme deactivation and prospective deactivation of enzyme remaining in the liquid phase were evaluated during wheat straw hydrolysis at 50 °C as a relevant saccharification process. Results suggest that the presence of a solid substrate dramatically reduces the global deactivation rate of the enzyme and, in addition, there is no loss the stability of the enzyme in the liquid phase along the saccharification process, even for 72 h. The authors gratefully acknowledge the funding granted by MINECO through contracts CTQ2013-45970-C2-1-R and PCIN-2013-021-C02-01 . Peer reviewed 2024-08-20T09:44:46Z 2024-08-20T09:44:46Z 2019-02-20 artículo http://purl.org/coar/resource_type/c_6501 Journal of Biotechnology 292: 32-38 (2019) 0168-1656 http://hdl.handle.net/10261/366105 10.1016/j.jbiotec.2019.01.009 http://dx.doi.org/10.13039/501100003329 30668958 2-s2.0-85060314099 https://api.elsevier.com/content/abstract/scopus_id/85060314099 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO//CTQ2013-45970-C2-1-R/ES/UTILIZACION EFICIENTE DE LA BIOMASA LIGNOCELULOSICA: BIORREFINERIA INTEGRADA/ info:eu-repo/grantAgreement/MINECO//PCIN-2013-021-C02-01/ES/PRODUCCION DE ACIDOS ORGANICOS PARA SINTESIS DE POLIESTERES/ Instituto de Ciencias Forestales (ICIFOR) Publisher's version https://doi.org/10.1016/j.jbiotec.2019.01.009 Sí open Elsevier
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic Cellobiose
Saccharification
Thermal stability
Wheat straw
β-glucosidase
Cellobiose
Saccharification
Thermal stability
Wheat straw
β-glucosidase
spellingShingle Cellobiose
Saccharification
Thermal stability
Wheat straw
β-glucosidase
Cellobiose
Saccharification
Thermal stability
Wheat straw
β-glucosidase
Wojtusik, M.
Vergara, Priscilla
Villar, J. C.
García-Ochoa, Félix
Ladero, Miguel
Thermal and operational deactivation of Aspergillus fumigatus β-glucosidase in ethanol/water pretreated wheat straw enzymatic hydrolysis
description 7 Pág.
author2 Ministerio de Economía y Competitividad (España)
author_facet Ministerio de Economía y Competitividad (España)
Wojtusik, M.
Vergara, Priscilla
Villar, J. C.
García-Ochoa, Félix
Ladero, Miguel
format artículo
topic_facet Cellobiose
Saccharification
Thermal stability
Wheat straw
β-glucosidase
author Wojtusik, M.
Vergara, Priscilla
Villar, J. C.
García-Ochoa, Félix
Ladero, Miguel
author_sort Wojtusik, M.
title Thermal and operational deactivation of Aspergillus fumigatus β-glucosidase in ethanol/water pretreated wheat straw enzymatic hydrolysis
title_short Thermal and operational deactivation of Aspergillus fumigatus β-glucosidase in ethanol/water pretreated wheat straw enzymatic hydrolysis
title_full Thermal and operational deactivation of Aspergillus fumigatus β-glucosidase in ethanol/water pretreated wheat straw enzymatic hydrolysis
title_fullStr Thermal and operational deactivation of Aspergillus fumigatus β-glucosidase in ethanol/water pretreated wheat straw enzymatic hydrolysis
title_full_unstemmed Thermal and operational deactivation of Aspergillus fumigatus β-glucosidase in ethanol/water pretreated wheat straw enzymatic hydrolysis
title_sort thermal and operational deactivation of aspergillus fumigatus β-glucosidase in ethanol/water pretreated wheat straw enzymatic hydrolysis
publisher Elsevier
publishDate 2019-02-20
url http://hdl.handle.net/10261/366105
http://dx.doi.org/10.13039/501100003329
https://api.elsevier.com/content/abstract/scopus_id/85060314099
work_keys_str_mv AT wojtusikm thermalandoperationaldeactivationofaspergillusfumigatusbglucosidaseinethanolwaterpretreatedwheatstrawenzymatichydrolysis
AT vergarapriscilla thermalandoperationaldeactivationofaspergillusfumigatusbglucosidaseinethanolwaterpretreatedwheatstrawenzymatichydrolysis
AT villarjc thermalandoperationaldeactivationofaspergillusfumigatusbglucosidaseinethanolwaterpretreatedwheatstrawenzymatichydrolysis
AT garciaochoafelix thermalandoperationaldeactivationofaspergillusfumigatusbglucosidaseinethanolwaterpretreatedwheatstrawenzymatichydrolysis
AT laderomiguel thermalandoperationaldeactivationofaspergillusfumigatusbglucosidaseinethanolwaterpretreatedwheatstrawenzymatichydrolysis
_version_ 1816136712745648128

Similar Items