Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds
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| Format: | artículo biblioteca |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2020-04-15
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| Subjects: | Alentejano pig, Bísaro pig, Dorsal subcutaneous fat, RNA-seq, Differentially expressed genes (DEGs), Transcriptome, |
| Online Access: | http://hdl.handle.net/10261/341958 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85083588286 |
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dig-inia-es-10261-3419582024-05-14T20:41:30Z Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds Albuquerque, André Óvilo Martín, Cristina Núñez, Yolanda Benítez Yáñez, Rita María López-García, Adrián García, Fabián Félix, Maria do Rosário Laranjo, Marta Charneca, Rui Martins, José Manuel European Commission Fundação para a Ciência e a Tecnologia (Portugal) Albuquerque, André [0000-0002-1189-9803] Óvilo Martín, Cristina [0000-0002-5738-8435] Núñez, Yolanda [0000-0001-5988-7628] Benítez Yáñez, Rita María [0000-0001-7459-2836] López-García, Adrián [0000-0002-8649-750X] Félix, Maria do Rosário [0000-0003-3870-5033] Laranjo, Marta [0000-0002-3900-5592] Charneca, Rui [0000-0002-3597-6746] Martins, José Manuel [0000-0003-3616-8406] Alentejano pig Bísaro pig Dorsal subcutaneous fat RNA-seq Differentially expressed genes (DEGs) Transcriptome 20 Pág. When compared to modern lean-type breeds, Portuguese local Alentejano (AL) and Bísaro (BI) pig breeds present a high potential for subcutaneous and intramuscular fat (IMF) deposition which contributes for better meat quality. The aim of this work was to explore the genome function to better understand the underlying physiological mechanisms associated with body fat accretion. Dorsal subcutaneous fat samples were collected at slaughter from adult animals (n = 4 for each breed) with ~150 kg body weight. Total RNA was obtained and sequenced for transcriptome analysis using DESeq2. A total of 458 differentially expressed (DE) genes (q-value < 0.05) were identified, with 263 overexpressed in AL and 195 in BI. Key genes involved in de novo fatty acid biosynthesis, elongation and desaturation were upregulated in AL such as ACLY, FASN, ME1, ELOVL6 and SCD. A functional enrichment analysis of the DE genes was performed using Ingenuity Pathway Analysis. Cholesterol synthesis is suggested to be higher in AL via SREBF2, SCAP and PPARG, while lipolytic activity may be more active in BI through GH and AMPK signalling. Increased signalling of CD40 together with the predicted activation of INSIG1 and INSIG2 in BI suggests that this breed is more sensitive to insulin whereas the AL is less sensitive like the Iberian breed. This work was funded by European Union’s H2020 RIA program (grant agreement no. 634476) and by Portuguese national funds through FCT/MCTES under project UIDB/05183/2020, and research grant SFRH/BD/132215/2017 to A. Albuquerque. Peer reviewed 2024-01-09T13:07:09Z 2024-01-09T13:07:09Z 2020-04-15 artículo http://purl.org/coar/resource_type/c_6501 Genes 11(4): e422 (2020) http://hdl.handle.net/10261/341958 10.3390/genes11040422 2073-4425 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100000780 32326415 2-s2.0-85083588286 https://api.elsevier.com/content/abstract/scopus_id/85083588286 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/634476 Departamento de Mejora Genética Animal Publisher's version https://doi.org/10.3390/genes11040422 Sí open application/pdf Multidisciplinary Digital Publishing Institute |
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Alentejano pig Bísaro pig Dorsal subcutaneous fat RNA-seq Differentially expressed genes (DEGs) Transcriptome Alentejano pig Bísaro pig Dorsal subcutaneous fat RNA-seq Differentially expressed genes (DEGs) Transcriptome |
| spellingShingle |
Alentejano pig Bísaro pig Dorsal subcutaneous fat RNA-seq Differentially expressed genes (DEGs) Transcriptome Alentejano pig Bísaro pig Dorsal subcutaneous fat RNA-seq Differentially expressed genes (DEGs) Transcriptome Albuquerque, André Óvilo Martín, Cristina Núñez, Yolanda Benítez Yáñez, Rita María López-García, Adrián García, Fabián Félix, Maria do Rosário Laranjo, Marta Charneca, Rui Martins, José Manuel Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds |
| description |
20 Pág. |
| author2 |
European Commission |
| author_facet |
European Commission Albuquerque, André Óvilo Martín, Cristina Núñez, Yolanda Benítez Yáñez, Rita María López-García, Adrián García, Fabián Félix, Maria do Rosário Laranjo, Marta Charneca, Rui Martins, José Manuel |
| format |
artículo |
| topic_facet |
Alentejano pig Bísaro pig Dorsal subcutaneous fat RNA-seq Differentially expressed genes (DEGs) Transcriptome |
| author |
Albuquerque, André Óvilo Martín, Cristina Núñez, Yolanda Benítez Yáñez, Rita María López-García, Adrián García, Fabián Félix, Maria do Rosário Laranjo, Marta Charneca, Rui Martins, José Manuel |
| author_sort |
Albuquerque, André |
| title |
Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds |
| title_short |
Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds |
| title_full |
Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds |
| title_fullStr |
Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds |
| title_full_unstemmed |
Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds |
| title_sort |
comparative transcriptomic analysis of subcutaneous adipose tissue from local pig breeds |
| publisher |
Multidisciplinary Digital Publishing Institute |
| publishDate |
2020-04-15 |
| url |
http://hdl.handle.net/10261/341958 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85083588286 |
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