Equilibration time improves the sperm variables of wild ruminant ejaculated and epididymal sperm cryopreserved by ultra-rapid freezing
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| Format: | artículo biblioteca |
| Language: | English |
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Academic Press
2023-08-24
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| Subjects: | Equilibration, Ibex, Mouflon, Sperm cryopreservation, Vitrification, |
| Online Access: | http://hdl.handle.net/10261/335019 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85169510097 |
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dig-inia-es-10261-3350192024-10-27T21:36:57Z Equilibration time improves the sperm variables of wild ruminant ejaculated and epididymal sperm cryopreserved by ultra-rapid freezing Martín, Adrián Castaño, Cristina O'Brien, Emma Toledano-Díaz, A. Guerra, Rafael Gómez-Guillamón, Félix Santiago Moreno, Julián Agencia Estatal de Investigación (España) Castaño, Cristina [0000-0003-1134-1436] Toledano Díaz, Adolfo [0000-0001-6679-485X] Gómez-Guillamón, Félix [0000-0001-9757-9589] Santiago Moreno, Julián [0000-0001-5551-8120] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] Equilibration Ibex Mouflon Sperm cryopreservation Vitrification 7 Pág. This work examines the effect of equilibration time with extender on ultra-rapidly frozen-thawed wild ruminant epididymal (origin: Iberian ibex) and ejaculated (origin: mouflon) sperm variables. Sperm samples were prepared either without prior equilibration, or equilibrated for 30 min before freezing. Higher quality (p < 0.05) frozen-thawed spermatozoa were obtained when equilibration was allowed, for ejaculated sperm in terms of sperm motility, acrosome apical ridge integrity, sperm viability, and percentage of normal cells, and for epididymal sperm in terms of linearity and straightness of sperm movement. The sperm head area, head perimeter, head length and head width were smaller (p < 0.01) in the equilibrated than non-equilibrated frozen-thawed epididymal sperm; no such dimensional changes were recorded for ejaculated sperm. In conclusion, equilibration prior to ultra-rapid freezing improves the cryoresistance of sperm cells, although viable sperm cells can be obtained without equilibration. The epididymal sperm showed greater cryoresistance, supporting the idea that it is more resistant to freeze-thawing than ejaculated sperm. This work was supported by grant PID2020-113288RB-I00/AEI/10.13039/501100011033. Peer reviewed 2023-09-13T09:37:47Z 2023-09-13T09:37:47Z 2023-08-24 artículo http://purl.org/coar/resource_type/c_6501 Cryobiology 113: e104579 (2023) 0011-2240 http://hdl.handle.net/10261/335019 10.1016/j.cryobiol.2023.104579 http://dx.doi.org/10.13039/501100011033 37633480 2-s2.0-85169510097 https://api.elsevier.com/content/abstract/scopus_id/85169510097 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113288RB-I00/ES/MECANISMOS DE CRIO-RESISTENCIA ESPERMATICA Y NUEVOS PROCEDIMIENTOS DE CRIOPRESERVACION ESPERMATICA Y TEJIDO TESTICULAR EN RUMIANTES DOMESTICOS Y SILVESTRES/ Departamento de Reproducción Animal Publisher's version https://doi.org/10.1016/j.cryobiol.2023.104579 Sí open application/pdf Academic Press |
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Biblioteca del INIA España |
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English |
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Equilibration Ibex Mouflon Sperm cryopreservation Vitrification Equilibration Ibex Mouflon Sperm cryopreservation Vitrification |
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Equilibration Ibex Mouflon Sperm cryopreservation Vitrification Equilibration Ibex Mouflon Sperm cryopreservation Vitrification Martín, Adrián Castaño, Cristina O'Brien, Emma Toledano-Díaz, A. Guerra, Rafael Gómez-Guillamón, Félix Santiago Moreno, Julián Equilibration time improves the sperm variables of wild ruminant ejaculated and epididymal sperm cryopreserved by ultra-rapid freezing |
| description |
7 Pág. |
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Agencia Estatal de Investigación (España) |
| author_facet |
Agencia Estatal de Investigación (España) Martín, Adrián Castaño, Cristina O'Brien, Emma Toledano-Díaz, A. Guerra, Rafael Gómez-Guillamón, Félix Santiago Moreno, Julián |
| format |
artículo |
| topic_facet |
Equilibration Ibex Mouflon Sperm cryopreservation Vitrification |
| author |
Martín, Adrián Castaño, Cristina O'Brien, Emma Toledano-Díaz, A. Guerra, Rafael Gómez-Guillamón, Félix Santiago Moreno, Julián |
| author_sort |
Martín, Adrián |
| title |
Equilibration time improves the sperm variables of wild ruminant ejaculated and epididymal sperm cryopreserved by ultra-rapid freezing |
| title_short |
Equilibration time improves the sperm variables of wild ruminant ejaculated and epididymal sperm cryopreserved by ultra-rapid freezing |
| title_full |
Equilibration time improves the sperm variables of wild ruminant ejaculated and epididymal sperm cryopreserved by ultra-rapid freezing |
| title_fullStr |
Equilibration time improves the sperm variables of wild ruminant ejaculated and epididymal sperm cryopreserved by ultra-rapid freezing |
| title_full_unstemmed |
Equilibration time improves the sperm variables of wild ruminant ejaculated and epididymal sperm cryopreserved by ultra-rapid freezing |
| title_sort |
equilibration time improves the sperm variables of wild ruminant ejaculated and epididymal sperm cryopreserved by ultra-rapid freezing |
| publisher |
Academic Press |
| publishDate |
2023-08-24 |
| url |
http://hdl.handle.net/10261/335019 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85169510097 |
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