Searching for links between environmental and clinical mecA+Staphylococcus aureus: A comparative genomics study
9 Pág.
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Elsevier BV
2023-06-24
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| Subjects: | Animals, Comparative genomics, Food, Humans, Water, MecA(+)Staphylococcus aureus, http://metadata.un.org/sdg/6, Ensure availability and sustainable management of water and sanitation for all, |
| Online Access: | http://hdl.handle.net/10261/332329 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85163486022 |
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Animals Comparative genomics Food Humans Water MecA(+)Staphylococcus aureus http://metadata.un.org/sdg/6 Ensure availability and sustainable management of water and sanitation for all Animals Comparative genomics Food Humans Water MecA(+)Staphylococcus aureus http://metadata.un.org/sdg/6 Ensure availability and sustainable management of water and sanitation for all |
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Animals Comparative genomics Food Humans Water MecA(+)Staphylococcus aureus http://metadata.un.org/sdg/6 Ensure availability and sustainable management of water and sanitation for all Animals Comparative genomics Food Humans Water MecA(+)Staphylococcus aureus http://metadata.un.org/sdg/6 Ensure availability and sustainable management of water and sanitation for all Rocha, Jaqueline Silva, Vanessa Poeta, Patrícia Botelho, João Manaia, Célia M. Searching for links between environmental and clinical mecA+Staphylococcus aureus: A comparative genomics study |
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9 Pág. |
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European Commission |
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European Commission Rocha, Jaqueline Silva, Vanessa Poeta, Patrícia Botelho, João Manaia, Célia M. |
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artículo |
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Animals Comparative genomics Food Humans Water MecA(+)Staphylococcus aureus http://metadata.un.org/sdg/6 Ensure availability and sustainable management of water and sanitation for all |
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Rocha, Jaqueline Silva, Vanessa Poeta, Patrícia Botelho, João Manaia, Célia M. |
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Rocha, Jaqueline |
| title |
Searching for links between environmental and clinical mecA+Staphylococcus aureus: A comparative genomics study |
| title_short |
Searching for links between environmental and clinical mecA+Staphylococcus aureus: A comparative genomics study |
| title_full |
Searching for links between environmental and clinical mecA+Staphylococcus aureus: A comparative genomics study |
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Searching for links between environmental and clinical mecA+Staphylococcus aureus: A comparative genomics study |
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Searching for links between environmental and clinical mecA+Staphylococcus aureus: A comparative genomics study |
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searching for links between environmental and clinical meca+staphylococcus aureus: a comparative genomics study |
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Elsevier BV |
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2023-06-24 |
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http://hdl.handle.net/10261/332329 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85163486022 |
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dig-inia-es-10261-3323292024-10-27T21:41:02Z Searching for links between environmental and clinical mecA+Staphylococcus aureus: A comparative genomics study Rocha, Jaqueline Silva, Vanessa Poeta, Patrícia Botelho, João Manaia, Célia M. European Commission Foundation for Science and Technology Fundação para a Ciência e a Tecnologia (Portugal) Rocha, Jaqueline [0000-0003-1651-9483] Silva, Vanessa [0000-0001-9406-8433] Poeta, Patrícia [0000-0003-0925-689X] Botelho, João [0000-0002-2771-2345] Manaia, Célia M. [0000-0002-3674-1789] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] Animals Comparative genomics Food Humans Water MecA(+)Staphylococcus aureus http://metadata.un.org/sdg/6 Ensure availability and sustainable management of water and sanitation for all 9 Pág. Staphylococcus aureus integrate the list of highly virulent and antibiotic resistant pathogens, mainly due to the mecA gene, associated with methicillin resistance. Given the ubiquity of this species, the aim of this study was to investigate whether closely related mecA+S. aureus found in the environment can be also thrive as clinical isolates and if the respective accessory genome may suggest bacterial adaptation. The genomes of environmental (water, animal facilities, food products, n = 111) isolates were compared with closely related genomes of clinical origin (human patients, n = 103). These genomes, available in the public database NCBI, were analysed for phylogeny, accessory genome, and presence of selected clinically relevant genes (n = 104). The genomes of environmental isolates belonged to 18 multi-locus sequence types (MLSTs), 11 of which also included clinical genomes, a result confirmed based on core-genome analysis. Genes significantly (p ≤ 0.05) more frequent among environmental genomes were related with resistance to β-lactams (blaI, blaPCI), aminoglycosides (ant(6)-Ia), macrolides (mph(C), erm(B)), enterotoxins (seg, sei, sem, sen, seo, seu) and serine protease functions (splB), among others. Genes significantly more frequent among clinical genomes were associated with resistance to macrolides (erm(C)), phenicols (fexA), fosfomycin (murA), the leucocidin virulence gene (lukS-PV), and serine protease functions (splA, splE). It is suggested that mecA+S. aureus can be exchanged between clinical and environmental settings, with accessory traits (particularly antibiotic resistance, virulence and stress response) possibly being associated with the habitat. The interplay between phylogeny and accessory genome is an interesting contribution to better understanding the ecology and evolution of mecA+S. aureus. This work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative theranostic biomarkers, with references NORTE-01-0145-FEDER-030101 and PTDC/SAUINF/30101/2017, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). This work was financially supported by FEDER through project RISK.AR “Assessing the risks associated with environmental antibiotic resistant bacteria: propagation and transmission to humans” (PTDC/CTA-AMB/28196/2017) – Programa Operacional Competitividade e Internacionalização. This work was also supported by the projects UIDB/CVT/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT). This work was supported by National Funds from FCT - Fundação para a Ciência e a Tecnologia through project UIDB/50016/2020. Peer reviewed 2023-08-02T09:59:52Z 2023-08-02T09:59:52Z 2023-06-24 artículo http://purl.org/coar/resource_type/c_6501 Science of the Total Environment 895: e165078 (2023) 0048-9697 http://hdl.handle.net/10261/332329 10.1016/j.scitotenv.2023.165078 1879-1026 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100000780 37356759 2-s2.0-85163486022 https://api.elsevier.com/content/abstract/scopus_id/85163486022 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC//PTDC/SAUINF/30101/2017 info:eu-repo/grantAgreement/EC//PTDC/CTA-AMB/28196/2017 Centro de Biotecnología y Genómica de Plantas (CBGP) Publisher's version https://doi.org/10.1016/j.scitotenv.2023.165078 Sí open application/pdf Elsevier BV |