Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis
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Frontiers Media
2023-02-15
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| Subjects: | Auxin regulated genes, Galls, Giant cells, Lateral root formation, Syncytia, |
| Online Access: | http://hdl.handle.net/10261/311758 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011698 https://api.elsevier.com/content/abstract/scopus_id/85149493267 |
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Auxin regulated genes Galls Giant cells Lateral root formation Syncytia Auxin regulated genes Galls Giant cells Lateral root formation Syncytia |
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Auxin regulated genes Galls Giant cells Lateral root formation Syncytia Auxin regulated genes Galls Giant cells Lateral root formation Syncytia Abril-Urias, Patricia Ruiz-Ferrer, Virginia Cabrera, Javier Olmo, Rocio Silva, Ana Cláudia Díaz-Manzano, Fernando Evaristo Domínguez-Figueroa, José Martínez-Gómez, Ángela Gómez-Rojas, Almudena Moreno-Risueño, Miguel Ángel Fenoll, Carmen Escobar, Carolina Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis |
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13 Pág. |
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Ministerio de Ciencia e Innovación (España) |
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Ministerio de Ciencia e Innovación (España) Abril-Urias, Patricia Ruiz-Ferrer, Virginia Cabrera, Javier Olmo, Rocio Silva, Ana Cláudia Díaz-Manzano, Fernando Evaristo Domínguez-Figueroa, José Martínez-Gómez, Ángela Gómez-Rojas, Almudena Moreno-Risueño, Miguel Ángel Fenoll, Carmen Escobar, Carolina |
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Auxin regulated genes Galls Giant cells Lateral root formation Syncytia |
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Abril-Urias, Patricia Ruiz-Ferrer, Virginia Cabrera, Javier Olmo, Rocio Silva, Ana Cláudia Díaz-Manzano, Fernando Evaristo Domínguez-Figueroa, José Martínez-Gómez, Ángela Gómez-Rojas, Almudena Moreno-Risueño, Miguel Ángel Fenoll, Carmen Escobar, Carolina |
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Abril-Urias, Patricia |
| title |
Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis |
| title_short |
Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis |
| title_full |
Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis |
| title_fullStr |
Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis |
| title_full_unstemmed |
Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis |
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divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in arabidopsis |
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Frontiers Media |
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2023-02-15 |
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http://hdl.handle.net/10261/311758 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011698 https://api.elsevier.com/content/abstract/scopus_id/85149493267 |
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dig-inia-es-10261-3117582024-05-16T20:45:27Z Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis Abril-Urias, Patricia Ruiz-Ferrer, Virginia Cabrera, Javier Olmo, Rocio Silva, Ana Cláudia Díaz-Manzano, Fernando Evaristo Domínguez-Figueroa, José Martínez-Gómez, Ángela Gómez-Rojas, Almudena Moreno-Risueño, Miguel Ángel Fenoll, Carmen Escobar, Carolina Ministerio de Ciencia e Innovación (España) Agencia Estatal de Investigación (España) Junta de Comunidades de Castilla-La Mancha European Commission Abril-Urias, Patricia [0000-0003-0677-5600] Ruiz-Ferrer, Virginia [0000-0002-7840-297X] Cabrera, Javier [0000-0002-9277-4876] Olmo, Rocio [0000-0002-4064-9504] Silva, Ana Cláudia [0000-0002-6113-9722] Díaz-Manzano, Fernando Evaristo [0000-0002-2064-5303] Domínguez-Figueroa, José [0000-0002-2614-557X] Martínez-Gómez, Ángela [0000-0002-8920-4725] Gómez-Rojas, Almudena [0000-0002-7397-4768] Moreno-Risueño, Miguel Ángel [0000-0002-9794-1450] Fenoll, Carmen [0000-0003-4653-6268] Auxin regulated genes Galls Giant cells Lateral root formation Syncytia 13 Pág. Cysts (CNs) and root-knot nematodes (RKNs) induce specialized feeding cells, syncytia, and giant cells (GCs), respectively, within plant roots. The plant tissues around the GCs usually by respond forming a root swelling called a gall that contains the GCs. The ontogenesis of feeding cells is different. GC formation is a process of new organogenesis from vascular cells, which are still not well characterized, that differentiate into GCs. In contrast, syncytia formation involves the fusion of adjacent cells that have already differentiated. Nonetheless, both feeding sites show an auxin maximum pertinent to feeding site formation. However, data on the molecular divergences and similarities between the formation of both feeding sites regarding auxin-responsive genes are still scarce. We studied genes from the auxin transduction pathways that are crucial during gall and lateral root (LR) development in the CN interaction by using promoter-reporter (GUS/LUC)transgenic lines, as well as loss of function lines of Arabidopsis. The promoters pGATA23 and several deletions of pmiR390a were active in syncytia, as were in galls, but pAHP6 or putative up-stream regulators as ARF5/7/19 were not active in syncytia. Additionally, none of these genes seemed to play a key role during cyst nematode establishment in Arabidopsis, as the infection rates in loss of function lines did not show significant differences compared to control Col-0 plants. Furthermore, the presence of only canonical AuxRe elements in their proximal promoter regions is highly correlated with their activation in galls/GCs (AHP6, LBD16), but those promoters active in syncytia (miR390, GATA23) carry AuxRe overlapping core cis-elements for other transcription factor families (i.e., bHLH, bZIP). Strikingly, in silico transcriptomic analysis showed very few genes upregulated by auxins common to those induced in GCs and syncytia, despite the high number of upregulated IAA responsive genes in syncytia and galls. The complex regulation of auxin transduction pathways, where different members of the auxin response factor (ARF) family may interact with other factors, and the differences in auxin sensitivity, as indicated by the lower induction of the DR5 sensor in syncytia than galls, among other factors, may explain the divergent regulation of auxin responsive genes in the two types of nematode feeding sites. This work was supported by the Spanish Government (PID2019-105924RB-I00, Ministerio de Ciencia e Innovacion, MCIN/AEI/10.13039/501100011033 and RED2018-102407-T), by the Castilla-La Mancha Government (SBPLY/17/180501/000287; SBPLY/21/180501/000033), o CE; and by MCIN of Spain and ERDF (grant PID2019-111523GB-I00) to MM-R. Peer reviewed 2023-06-16T17:30:07Z 2023-06-16T17:30:07Z 2023-02-15 artículo http://purl.org/coar/resource_type/c_6501 Frontiers in Plant Science 14: e1024815 (2023) 1664-462X http://hdl.handle.net/10261/311758 10.3389/fpls.2023.1024815 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011698 36875577 2-s2.0-85149493267 https://api.elsevier.com/content/abstract/scopus_id/85149493267 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105924RB-I00/ES/ESTUDIANDO LOS SITIOS DE ALIMENTACION (AGALLAS%2FCELULAS GIGANTES) DE NEMATODOS FITOPARASITOS CON UNA PERSPECTIVA CELULAR PARA EL DESARROLLO DE HERRAMIENTAS DIRIGIDAS DE CONTROL/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111523GB-I00/ES/MECANISMOS REGULADORES Y MOLECULAS SEÑALIZADORAS DURANTE LA FORMACION POSTEMBRIONICA DE RAICES/ Centro de Biotecnología y Genómica de Plantas (CBGP) Publisher's version https://doi.org/10.3389/fpls.2023.1024815 Sí open application/pdf Frontiers Media |