Assesment of mitocondrial redox activity during cryopreservation process of indian red jungle fowl (Gallus Gallus Murghi) sperm through MTT reduction assay
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Pakistan Agricultural Scientists Forum
2023-02-22
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| Subjects: | DNA integrity, Indian red jungle fowl, Metabolic redox activity, MTT Reduction, |
| Online Access: | http://hdl.handle.net/10261/311067 https://api.elsevier.com/content/abstract/scopus_id/85149248877 |
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dig-inia-es-10261-3110672024-10-28T22:02:45Z Assesment of mitocondrial redox activity during cryopreservation process of indian red jungle fowl (Gallus Gallus Murghi) sperm through MTT reduction assay Rakha, Bushra A. Ansari, M. S. Akhter, Shamim Akhter, Ali Blesbois, E. Moreno, Julián Santiago Blesbois, E. [0000-0001-8182-6602] Moreno, Julián Santiago [0000-0001-5551-8120] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] DNA integrity Indian red jungle fowl Metabolic redox activity MTT Reduction 6 Pág. The experiment was conducted to evaluate the metabolic activity through MTT (3[4,5-dimethylthiazol-2-y1]-2,5-diphenyltetrazolium bromide) reduction assay of Indian red jungle fowl sperm during cryopreservation process. In addition, MTT test was compared with sperm motility and viability data. Sperm analysis was made at pre-dilution, post-dilution, post-cooling, post-equilibration and post freeze-thawing stages. Pearson’s correlation coefficient showed significant (P<0.01) positive association of MTT reduction rate with motility (r=0.85) and viability (r=0.84). A significant (P<0.05) decline was observed in motility, viability and metabolic status after eac h stage of cryopreservation. The highest (P<0.05) stage-specific percent decrease in motility and viability were observed after freeze-thawing. Nevertheless, stage-specific percent decrease in metabolic status differ (P<0.05) after dilution (5.93 ± 0.12), cooling (8.41 ± 0.03), equilibration (19.1 ± 0.04) and freeze-thawing (30.9 ± 0.08). The total decrease in motility, viability and metabolic activity after the complete freeze-thawing cycle were calculated as 56%, 47% and 52%, respectively. It is concluded that MTT reduction assay is simple and reliable method to evaluate mitochondrial redox activity in sperm of this threatened species. Peer reviewed 2023-06-09T08:21:18Z 2023-06-09T08:21:18Z 2023-02-22 artículo http://purl.org/coar/resource_type/c_6501 Journal of Animal and Plant Sciences 33(1): 229-234 (2023) 1018-7081 http://hdl.handle.net/10261/311067 10.36899/JAPS.2023.1.0613 2309-8694 2-s2.0-85149248877 https://api.elsevier.com/content/abstract/scopus_id/85149248877 en Departamento de Reproducción Animal Publisher's version https://doi.org/10.36899/JAPS.2023.1.0613 Sí open application/pdf Pakistan Agricultural Scientists Forum |
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DNA integrity Indian red jungle fowl Metabolic redox activity MTT Reduction DNA integrity Indian red jungle fowl Metabolic redox activity MTT Reduction |
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DNA integrity Indian red jungle fowl Metabolic redox activity MTT Reduction DNA integrity Indian red jungle fowl Metabolic redox activity MTT Reduction Rakha, Bushra A. Ansari, M. S. Akhter, Shamim Akhter, Ali Blesbois, E. Moreno, Julián Santiago Assesment of mitocondrial redox activity during cryopreservation process of indian red jungle fowl (Gallus Gallus Murghi) sperm through MTT reduction assay |
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6 Pág. |
| author2 |
Blesbois, E. [0000-0001-8182-6602] |
| author_facet |
Blesbois, E. [0000-0001-8182-6602] Rakha, Bushra A. Ansari, M. S. Akhter, Shamim Akhter, Ali Blesbois, E. Moreno, Julián Santiago |
| format |
artículo |
| topic_facet |
DNA integrity Indian red jungle fowl Metabolic redox activity MTT Reduction |
| author |
Rakha, Bushra A. Ansari, M. S. Akhter, Shamim Akhter, Ali Blesbois, E. Moreno, Julián Santiago |
| author_sort |
Rakha, Bushra A. |
| title |
Assesment of mitocondrial redox activity during cryopreservation process of indian red jungle fowl (Gallus Gallus Murghi) sperm through MTT reduction assay |
| title_short |
Assesment of mitocondrial redox activity during cryopreservation process of indian red jungle fowl (Gallus Gallus Murghi) sperm through MTT reduction assay |
| title_full |
Assesment of mitocondrial redox activity during cryopreservation process of indian red jungle fowl (Gallus Gallus Murghi) sperm through MTT reduction assay |
| title_fullStr |
Assesment of mitocondrial redox activity during cryopreservation process of indian red jungle fowl (Gallus Gallus Murghi) sperm through MTT reduction assay |
| title_full_unstemmed |
Assesment of mitocondrial redox activity during cryopreservation process of indian red jungle fowl (Gallus Gallus Murghi) sperm through MTT reduction assay |
| title_sort |
assesment of mitocondrial redox activity during cryopreservation process of indian red jungle fowl (gallus gallus murghi) sperm through mtt reduction assay |
| publisher |
Pakistan Agricultural Scientists Forum |
| publishDate |
2023-02-22 |
| url |
http://hdl.handle.net/10261/311067 https://api.elsevier.com/content/abstract/scopus_id/85149248877 |
| work_keys_str_mv |
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